FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709282
Sequences flagged as poor quality0
Sequence length21-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29610.4174644217673647No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAGAGTACAATTGTGCTTGTATATGAGCTGCAATAATAATCAG26420.3724893624820593No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25160.354724919002597No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAGAGTAC24700.34823948725612663No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22530.31764516793038594No Hit
CCTATGAACATCCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21180.298611835630962No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG19600.2763357874583029No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC18580.26195504749873816No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17480.24644640636587423No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17440.2458824557792246No Hit
GTAGTATATGGCCGTGTCTTCGGCTCTCAGACTATTCATTTCCAGATACA15820.2230424570199159No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGTCGAT15470.21810788938673195No Hit
GAGTACAATTGTGCTTGTATATGAGCTGCAATAATAATCAGCCTCGTCCT15320.21599307468679593No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCTCATAT15210.21444221057350957No Hit
GTACGGAGGTATGCACGTCTGGGGCCGAGGGACCACGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGT14880.20978961823365036No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14610.20598295177376558No Hit
CCTCCGTACCACTCGAAAAATCGTCGCTCTTTCGTACAGTAGTATATGGC14580.2055599888337784No Hit
ATATACTACTGTACGAAAGAGCGACGATTTTTCGAGTGGTACGGAGGTAT14240.2007664088472568No Hit
CAATTGTACTCTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC13940.19653677944738482No Hit
ATTGTACTCTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC13700.19315307592748723No Hit
CAACATATACTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAAGCAATTCCATGCTCACACTGTAT13660.19258912534083764No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13570.19132023652087604No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13390.18878245888095285No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13200.18610369359436726No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13150.18539875536105527No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12880.18159208890117046No Hit
GTATATGTTGTCACCATTGCTATTTATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT12750.1797592494945593No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12530.1766575212679865No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12400.17482468186137531No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12350.17411974362806332No Hit
CTCCTGCACTGGAACCAACAGTGTCGATTATAACTATGTCTCCTGGTACC12220.1722869042214521No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12120.17087702775482813No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12110.17073604010816573No Hit
GTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12030.1696081389348665No Hit
GCCATATACTACTGTACGAAAGAGCGACGATTTTTCGAGTGGTACGGAGG11990.16904418834821694No Hit
GTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11810.16650641070829372No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGCGGTGGTCTCCA11790.16622443541496895No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAGAGTACAATTGTGCTTGTATATGAGCTGCAATAATAATCAGC11770.16594246012164415No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGTCGATTATAACTATGTCTCCTGGTACCAACAGCACCCAGG11560.16298171954173374No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11510.16227678130842177No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11290.15917505308184898No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11100.15649628779526337No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGA10660.1502928313421178No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10660.1502928313421178No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10570.14902394252215623No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGGGACTGACTACACCTTTAGCAACTATGCCATGAACTGGGTCCG10330.14564023900225861No Hit
GTATATGGCCGTGTCTTCGGCTCTCAGACTATTCATTTCCAGATACAGTG10280.14493530076894662No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9990.14084665901573704No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9870.13915480725578824No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9780.13788591843582665No Hit
GTACAATTGTGCTTGTATATGAGCTGCAATAATAATCAGCCTCGTCCTCA9680.13647604196920265No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATCC9350.13182344962934348No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9270.13069554845604428No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9270.13069554845604428No Hit
GATTATTATTGCAGCTCATATACAAGCACAATTGTACTCTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAG9250.13041357316271948No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAG9240.13027258551605708No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC9160.1291446843427579No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC9150.1290036966960955No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9110.1284397461094459No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9070.1278757955227963No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8980.1266069067028347No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATCCTGTAGGGGC8840.12463307964956111No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG8470.11941653672305232No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8320.11730172202311634No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8310.11716073437645393No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8230.11603283320315474No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8150.11490493202985554No Hit
CAGTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAGGTCTGGCAACACGGCCTCCCTGACCATCT8150.11490493202985554No Hit
CCACCGCACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT8130.11462295673653074No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8080.11391801850321875No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC8030.11321308026990674No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8010.11293110497658196No Hit
GGAACCAACAGTGTCGATTATAACTATGTCTCCTGGTACCAACAGCACCC8000.11279011732991955No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAGAGTACAATTGTG7960.11222616674326996No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7920.11166221615662036No Hit
GGTACCAACAGCACCCAGGCAACGCCCCCAAACTCATAATTTATGAGCTC7900.11138024086329555No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT7870.11095727792330835No Hit
GTTGAGAACCTATGAACATCCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7850.11067530262998355No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7820.11025233968999637No Hit
ATCCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC7740.10912443851669716No Hit
GTATATGAGCTGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA7690.10841950028338516No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG7590.10700962381676117No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7560.10658666087677397No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCC7520.10602271029012438No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7500.10574073499679958No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC7430.10475382147016278No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCT7380.10404888323685078No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT7330.1033439450035388No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG7260.102357031476902No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7140.10066517971695321No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7100.1001012291303036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC800.056.08599541
TCGTATG800.056.00202640
AGGGGGG852.4348992E-855.1540570
TCTCGTA751.8189894E-1145.82949438
CACGAGA900.045.44497321
GCATAAG306.703394E-445.438561
GCCGTCT1000.045.19145246
ATCTCGT803.6379788E-1142.92547237
TATACAC1050.042.192953
ACGAGAC1150.041.49323322
AGACCGA1100.040.30065525
GTATCAA10750.039.9436651
GGTATCA3950.039.6868481
ATGCCGT1150.039.17577744
CGAGCCC1050.038.95557815
GAGACCG1150.038.53758224
ACACATC1150.038.5267146
GAAATGT700.001559128238.27015770
CTATTAA700.001559128238.27015770
CCGTCTT1200.037.72193547
Produced by FastQC (version 0.11.5)