FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709282
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC28130.39659825006132965No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAGAGTACAATTGTGCTTGTATATGAGCTGCAATAATAATCAG24100.33978022845638267No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAGAGTAC22890.3227207232102323No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22150.31228763735721476No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG21640.3050972673774324No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21440.3022775144441844No Hit
CCTATGAACATCCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT18460.26026319573878937No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16890.23812813521279264No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16680.23516739463288225No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCTCATAT16090.2268491234798007No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC15900.22417035819321512No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15660.22078665467331754No Hit
GTAGTATATGGCCGTGTCTTCGGCTCTCAGACTATTCATTTCCAGATACA15180.21401924763352234No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG14990.21134048234693675No Hit
GAGTACAATTGTGCTTGTATATGAGCTGCAATAATAATCAGCCTCGTCCT14600.20584196412710315No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGTCGAT14500.20443208766047918No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14420.20330418648718No Hit
CAATTGTACTCTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC14050.1980876435606712No Hit
ATTGTACTCTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTC13750.19385801416079923No Hit
GTACGGAGGTATGCACGTCTGGGGCCGAGGGACCACGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGT13660.19258912534083764No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13620.19202517475418804No Hit
CCTCCGTACCACTCGAAAAATCGTCGCTCTTTCGTACAGTAGTATATGGC13470.18991036005425205No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT13410.18906443417427765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13210.18624468124102966No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13080.18441184183441847No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12780.1801822124345465No Hit
ATATACTACTGTACGAAAGAGCGACGATTTTTCGAGTGGTACGGAGGTAT12750.1797592494945593No Hit
GTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12640.1782083853812729No Hit
GTATATGTTGTCACCATTGCTATTTATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTT12340.17397875598140092No Hit
CTCCTGCACTGGAACCAACAGTGTCGATTATAACTATGTCTCCTGGTACC12340.17397875598140092No Hit
CAACATATACTACGCAGACTCCGTGAAGGGCCGCTTCACCATCTCCAGAAGCAATTCCATGCTCACACTGTAT12270.17299184245476412No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG12070.17017208952151613No Hit
GTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11720.16523752188833213No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11620.16382764542170813No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11190.15776517661522496No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGCGGTGGTCTCCACTCCCGCCTTGA11180.15762418896856256No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGTCGATTATAACTATGTCTCCTGGTACCAACAGCACCCAGG11140.157060238381913No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11080.1562143125019386No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11080.1562143125019386No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGCGGTGGTCTCCA10890.153535547215353No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10870.15325357192202818No Hit
GTACAATTGTGCTTGTATATGAGCTGCAATAATAATCAGCCTCGTCCTCA10500.1480370289955194No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGAAGGGACTGACTACACCTTTAGCAACTATGCCATGAACTGGGTCCG10460.1474730784088698No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10380.1463451772355706No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10230.14423036253563465No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10180.14352542430232262No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10100.14239752312902343No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9980.14070567136907464No Hit
GTATATGGCCGTGTCTTCGGCTCTCAGACTATTCATTTCCAGATACAGTG9940.14014172078242504No Hit
GCCATATACTACTGTACGAAAGAGCGACGATTTTTCGAGTGGTACGGAGG9840.13873184431580105No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT9800.13816789372915145No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC9700.13675801726252745No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCAAAGAGTACAATTGTGCTTGTATATGAGCTGCAATAATAATCAGC9650.13605307902921546No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9530.13436122726926666No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9490.13379727668261707No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9220.12999061022273228No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATCC9120.1285807337561083No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9100.1282987584627835No Hit
GGAACCAACAGTGTCGATTATAACTATGTCTCCTGGTACCAACAGCACCC8850.12477406729622349No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8660.1220953020096379No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCCAGAGAAG8500.11983949966303951No Hit
GATTATTATTGCAGCTCATATACAAGCACAATTGTACTCTTTGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAG8470.11941653672305232No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC8150.11490493202985554No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC8130.11462295673653074No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC8130.11462295673653074No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8080.11391801850321875No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG7960.11222616674326996No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG7960.11222616674326996No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCAGCT7920.11166221615662036No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA7900.11138024086329555No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATCCTGTAGGGGC7770.10954740145668437No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7720.10884246322337236No Hit
CCACCGCACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT7620.10743258675674837No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC7580.10686863617009879No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7580.10686863617009879No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC7490.10559974735013718No Hit
CAGTAATCGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAGGTCTGGCAACACGGCCTCCCTGACCATCT7480.10545875970347479No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC7420.1046128338235004No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT7370.10390789559018838No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT7340.10348493265020119No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACCTGGAGCC7300.10292098206355157No Hit
GTATATGAGCTGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA7290.1027799944168892No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC7240.10207505618357719No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC7200.10151110559692761No Hit
GGTACCAACAGCACCCAGGCAACGCCCCCAAACTCATAATTTATGAGCTC7130.1005241920702908No Hit
GTTGAGAACCTATGAACATCCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7110.10024221677696599No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC7100.1001012291303036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA150.002300102369.412845
TATAGCT200.00777356451.0355874
GTGGTAT1550.043.9263571
GTATCAA12700.042.620671
AATGGTA1950.042.02943870
GGTATCA4550.041.150791
TGGTATC1700.040.0307352
GTAATAC350.001442755338.8842543
TATCCTC350.001442755338.8842545
TATCAAC14300.037.5952952
ATCAACG15000.035.6114963
AACGCAG15200.035.150366
TCAACGC15150.035.034334
CAACGCA15350.034.5778585
GTGTATA400.002771075634.0429231
CCATTAA400.002771075634.0429231
CGCAGAG16400.032.3800138
CAGAGTA16550.032.09559210
GCAGAGT16950.031.3359629
CCCGTCT450.004927920630.26678317
Produced by FastQC (version 0.11.5)