FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962223
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT38940.4046878945940806No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35240.36623526978673343No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCCTATACAGTGATGGAAACACCTACTTGA32050.3330828716420206No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31220.32445701256361575No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26670.277170676651878No Hit
CTCCTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCC26560.27602749050895686No Hit
CCTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAG25330.2632445909108388No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23700.24630465079300742No Hit
GTATAGGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCCAAGGGTGACGGGCAGGGAGAGTGGAGA23150.24058872007840176No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23130.24038086805241612No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22950.2385101998185452No Hit
CTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCA22770.23663953158467424No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22550.23435315929883196No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22430.23310604714291802No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22000.2286372285842263No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG21440.22281737185662784No Hit
GTACACATTGGCCACACACTTTCGGCCCTGGGACCAAAGTGGATATCAAA20270.2106580283364667No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19790.20566957971281086No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19470.2023439472970403No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19460.20224002128404744No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19190.19943401893324103No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA18800.19538090442652067No Hit
ATGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC18540.19267882808870707No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC18320.19039245580286482No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18300.19018460377687915No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC18260.18976889972490785No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA18090.1880021575040297No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC17910.1861314892701588No Hit
GTAATATACGGCCGTGTCCTCGACTCTCAGGCTGTTCAGTTGCAGATACA17820.18519615515322332No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17010.17677814810080408No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAACATCC16380.17023080928225579No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG16120.1675287329444422No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16000.16628162078852823No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15500.1610853201388867No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15200.15796753974910183No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15180.15775968772311616No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT14740.15318694315143164No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14640.15214768302150333No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14640.15214768302150333No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14550.15121234890456786No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14430.1499652367486539No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA14360.14923775465770409No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC13850.14393752799506976No Hit
GTTTATTACTGCATGCAAGGTACACATTGGCCACACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA13780.14321004590411993No Hit
GGCCGAAAGTGTGTGGCCAATGTGTACCTTGCATGCAGTAATAAACCCCA13630.14165115570922748No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCCTATA13550.14081974760528485No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13360.13884515335842107No Hit
CTGTATAGGAGGCTTTGACTAGACCTGCAGGAGATGGAGGCCGGCTGTCC13010.13520774290367202No Hit
GTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTT12760.13260959257885127No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12670.13167425846191577No Hit
GGTCTAGTCAAAGCCTCCTATACAGTGATGGAAACACCTACTTGACTTGGTTTCAGCAGAGGCCAGGCCAATCTC12640.1313624804229373No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT12580.13073892434498033No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12370.1285564780721309No Hit
TTATTACTGCATGCAAGGTACACATTGGCCACACACTTTCGGCCCTGGGA12090.12564654970833164No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG12050.12523084565636033No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11970.12439943755241768No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11910.12377588147446071No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11750.12211306526657544No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11730.12190521324058977No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11730.12190521324058977No Hit
GGTACACATTGGCCACACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT11730.12190521324058977No Hit
AGGTACACATTGGCCACACACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGGCTGCACCAT11700.12159343520161128No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11660.12117773114963995No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11590.12045024905869013No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11530.11982669298073315No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT11450.11899528487679051No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11340.11785209873386938No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11260.11702069062992675No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11200.11639713455196976No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11140.11577357847401278No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG11120.11556572644802712No Hit
GTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCT11080.11515002239605579No Hit
GTATATTACTGTGCGAGAGACCCCGCCTTTGGGGCTATTGACTACTGGGGCCCGGGAACCCTGGTCACCGTCTC11080.11515002239605579No Hit
AATGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACC10910.1133832801751777No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10910.1133832801751777No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG10810.11234402004524939No Hit
GTTTCTAACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTC10810.11234402004524939No Hit
GGCCTGGCCTCTGCTGAAACCAAGTCAAGTAGGTGTTTCCATCACTGTAT10660.11078512985035693No Hit
CTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTA10660.11078512985035693No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10560.10974586972042864No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10560.10974586972042864No Hit
GGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGACTCTGG10530.10943409168145013No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT10340.10745949743458637No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10330.10735557142159355No Hit
GATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGGTACACATTGG10320.1072516454086007No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10290.10693986736962222No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA10220.10621238527867241No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10170.10569275521370826No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10170.10569275521370826No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT9970.10361423495385165No Hit
GCAGTGGGTCAGGCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCT9900.10288675286290186No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA9850.10236712279793769No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9840.10226319678494486No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9730.10112001064202372No Hit
AACCGGGACTCTGGGGTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCAC9690.10070430659005242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGTCG200.006873751552.6497852
CTTATAC2300.051.7889561
CGGACGA200.007341194451.78107541
GGTATCA8350.048.0941731
TCGTATG2350.046.94012540
CGTATGC2450.046.4972941
CCGTCTT2350.045.91311347
ATGCCGT2350.045.72356444
ATCTCGT2200.045.2612237
TATACAC2650.044.9489063
ACGAGAC2500.044.9442422
GCCGTCT2400.044.88502546
CCCACGA2450.044.4578619
CTCTCTA2550.044.1709229
GTATGCC2600.043.8864742
CATCTCG2300.043.25251836
CACGAGA2550.042.72107321
CGTCTTC2450.042.67292448
ATCTCCG2800.042.54092410
TCCGAGC2750.042.0724713
Produced by FastQC (version 0.11.5)