FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162493
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46110.3966475497056757No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43130.3710129867448664No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34890.30013083949752817No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33120.28490494136308775No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31050.2670983825278948No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30800.2649478319439343No Hit
GTATGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT29210.2512703302299455No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29170.2509262421365118No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC29050.24989397785621076No Hit
GTATGAAAGCTGGCCCAGGTGTACCTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25200.21677549886321895No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24040.20679694415364222No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23990.2063668340368501No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT23500.2021517548922875No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23230.19982916026161016No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23170.19931302812145965No Hit
GTGTATATGGAGCTGACCAACTTAACATCTGACGACACGGCCCTATATTA23070.19845280788787545No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTGCTCTGGCTCCCAGATACCA22270.19157104601920183No Hit
GTCCCATACCCACTGCCACTGAACCTGACTGGGATATCAGTGGCCCTGAT20440.17582901574461093No Hit
CTGATATCCCAGTCAGGTTCAGTGGCAGTGGGTATGGGACAGACTTCACT19890.17109780445989783No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19810.17040962827303047No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19650.16903327589929573No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19620.16877520982922048No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19390.1667967032919768No Hit
CCATATACACTGTGCCCGTGAACTCGTCCGCGGTAATTGTCAGTCTGTCCCGGAAAGTCTGTGCGTAGTTTG19360.16653863722190154No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18950.16301173426420634No Hit
GGTGTACCTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC18540.1594848313065111No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18260.15707621465247534No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG17420.14985036469036803No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17160.14761379208304912No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTTTGAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT17100.14709765994289858No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTTTGAG16960.1458933516158807No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT16910.1454632414990886No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG16610.142882580798336No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16540.14228042663482704No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16530.14219440461146865No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16440.14142020640124284No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC16430.14133418437788445No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16350.14064600819101708No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15920.136947061186605No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15580.1340223123924187No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAAGGTA15490.1332481141821929No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15250.13118358562159085No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC15210.13083949752815716No Hit
TCTCTGGTGCATCCATCAGGGCCACTGATATCCCAGTCAGGTTCAGTGGCAGTGGGTATGGGACAGACTTCACT14950.12860292492083825No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14870.12791474873397088No Hit
CTATATTACTGTGCGAAAGAGCCGAGTGATGGAAGTGGTTCTTACTTTGA14630.12585022017336878No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG14570.12533408803321827No Hit
GGGTATGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGA14410.12395773565948354No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCATTTTATTACTGTCAACAGTATGAAAGCTGG14360.12352762554269145No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14330.12326955947261618No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14060.12094696484193884No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13940.11991470056163778No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13760.11836630414118622No Hit
GCTTTCATACTGTTGACAGTAATAAAATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGT13680.11767812795431887No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTTTGAGCAACTTAGCCTGGTA13550.11655984165065941No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13530.11638779760394255No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13330.11466735713677415No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13260.11406520297326521No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA13210.11363509285647311No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13160.113204982739681No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12950.11139852024915418No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12930.11122647620243735No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12900.11096841013236208No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTGCTCTGGCTCCCAGATACCACTGGAGAAATAGTG12870.11071034406228682No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12760.10976410180534421No Hit
ATATTACTGTGCGAAAGAGCCGAGTGATGGAAGTGGTTCTTACTTTGACACCTGGGGCCAGGGAACCCTGGTCGC12720.10942001371191051No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12530.10778559526810054No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12470.10726946312795002No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12370.10640924289436582No Hit
ACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC12330.10606515480093215No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12280.10563504468414005No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12120.10425869231040531No Hit
GGATATCAGTGGCCCTGATGGATGCACCAGAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG12030.10348449410017951No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11990.10314040600674584No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11880.10219416374980322No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11850.10193609767972796No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG11850.10193609767972796No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC11850.10193609767972796No Hit
GTAATATAGGGCCGTGTCGTCAGATGTTAAGTTGGTCAGCTCCATATACA11820.1016780316096527No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11810.10159200958629429No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC11760.10116189946950219No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11670.10038770125927642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)