FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162493
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44750.3849485545289305No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38490.3310987679065594No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35990.3095932620669544No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33350.28688344790033143No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30780.26477578789721745No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28350.24387243622112134No Hit
GTATGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATT27910.24008746719335083No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26900.23139924283415042No Hit
GTATGAAAGCTGGCCCAGGTGTACCTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT25470.2190980934938963No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24380.2097216929478285No Hit
GTGTATATGGAGCTGACCAACTTAACATCTGACGACACGGCCCTATATTA24210.20825931855073537No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23740.2042162834528896No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23640.2033560632193054No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23520.20232379893900437No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTGCTCTGGCTCCCAGATACCA22160.1906248037622592No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21370.18382906391694404No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT20900.17978602881909828No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20580.17703332407162883No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20340.17496879551102673No Hit
GTCCCATACCCACTGCCACTGAACCTGACTGGGATATCAGTGGCCCTGAT19960.17169995862340676No Hit
GGTGTACCTTCGGCCAAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC18810.16180742593718844No Hit
CTGATATCCCAGTCAGGTTCAGTGGCAGTGGGTATGGGACAGACTTCACT18700.1608611836802458No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18210.15664610453568323No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18180.15638803846560798No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18060.15535577418530694No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17580.15122671706410273No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17450.15010843076044328No Hit
CCATATACACTGTGCCCGTGAACTCGTCCGCGGTAATTGTCAGTCTGTCCCGGAAAGTCTGTGCGTAGTTTG17350.14924821052685908No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTTTGAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT17270.14856003433999174No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTTTGAG16700.14365677900856177No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16440.14142020640124284No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16390.14099009628445075No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT16050.13806534749026445No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT15870.1365169510698129No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15740.13539866476615345No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15550.13376424632234343No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15470.13307607013547607No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTTGGCCGAAGGTA15220.13092551955151557No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15180.1305814314580819No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15020.12920507908434717No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG14900.12817281480404613No Hit
TCTCTGGTGCATCCATCAGGGCCACTGATATCCCAGTCAGGTTCAGTGGCAGTGGGTATGGGACAGACTTCACT14760.12696850647702823No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCATTTTATTACTGTCAACAGTATGAAAGCTGG14460.12438784577627564No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14090.12120503091201408No Hit
GGGTATGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGA14060.12094696484193884No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13960.12008674460835464No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13760.11836630414118622No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13740.11819426009446939No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13590.11690392974409308No Hit
CTATATTACTGTGCGAAAGAGCCGAGTGATGGAAGTGGTTCTTACTTTGA13480.11595768748715045No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13420.11544155534699994No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13240.11389315892654837No Hit
ACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATC13210.11363509285647311No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTTTGAGCAACTTAGCCTGGTA13160.113204982739681No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13120.11286089464624734No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC13050.11225874048273839No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC13040.11217271845937998No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12880.11079636608564525No Hit
GCTTTCATACTGTTGACAGTAATAAAATGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAGT12860.11062432203892841No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG12680.10907592561847684No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12650.10881785954840158No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12610.10847377145496791No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACCATCGACCGCTTGGCGGGTAAACC12550.10795763931481737No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA12370.10640924289436582No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12220.10511891254398952No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12190.10486084647391425No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12140.10443073635712215No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12050.10365653814689638No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12050.10365653814689638No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11880.10219416374980322No Hit
ATATTACTGTGCGAAAGAGCCGAGTGATGGAAGTGGTTCTTACTTTGACACCTGGGGCCAGGGAACCCTGGTCGC11870.1021081417264448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAT150.002612805767.2195614
AGTATCG200.006470524753.46137653
GGTATCA10050.044.434591
GTATCAA25350.038.4111
GATGGAA13050.032.27495670
ACGACAC7600.031.73765432
TAACGGC652.4677362E-531.56280936
CGACACG7700.031.37876133
GTGTATA8150.031.3106121
ACGGCCC7800.031.16814237
TGTTAGA17400.031.06464270
TGACGAC7850.031.02593630
CTGACGA7950.031.0208229
GACGACA7800.030.85535231
TAACATC8100.030.756923
GGCCCTA8150.030.40052639
TATCAAC32300.029.823972
ATCAACG32250.029.7661323
TAACCGT604.4817623E-429.70076653
ATGGCAA2601.6189006E-1029.69916570
Produced by FastQC (version 0.11.5)