FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529867
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTATACTACTCCTCAGACGTTCGGCCAAGGGACTAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT27690.5225839691847199No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18870.35612710359392075No Hit
GTATAATGTTGCTGACAATAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC16460.31064399179416724No Hit
GTATATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTA16320.308001819324472No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16220.30611455327468967No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14510.2738423038234123No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12480.23553080301283152No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG12090.22817046541868052No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11450.2160919627000738No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11270.21269488381046564No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11190.2111850709706398No Hit
CTCATATACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGACCGATTCAG10980.20722181226609698No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10530.1987291150420766No Hit
GGACTAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC10260.1936334967076644No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10110.1908025976329909No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9790.18476334627368754No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGACCTGGATAAAACATT9620.1815549939890576No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9110.17192993713516788No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8870.16740049861569034No Hit
CTCCTAAGTTGCTCATATACTGGGCATCTGTCCGGGAATCCGGGGTCCCT8820.1664568655907992No Hit
GTTTTATCCAGGTCCAACAATAAGAACTACTTAGCTTGGTACCAACAGAA8670.16362596651612574No Hit
GTAATACACAGCCGTGTCGTCAGTTTTCAGGCTGTTCATTTGTAGAAACA8560.16154997386136521No Hit
TTATACTACTCCTCAGACGTTCGGCCAAGGGACTAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT8200.15475581608214892No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8180.15437836287219245No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7840.14796165830293262No Hit
ATACTACTCCTCAGACGTTCGGCCAAGGGACTAAGGTGGAAATCAAACGA7660.14456457941332448No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC7470.14097877391873812No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7470.14097877391873812No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7370.1390915078689558No Hit
GCTCATATACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT7340.1385253280540211No Hit
CCTTAGTCCCTTGGCCGAACGTCTGAGGAGTAGTATAATGTTGCTGACAATAATAAACTGCCACATCTTCAGG7240.1366380620042388No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7210.1360718821893041No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7120.13437334274450002No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6970.1315424436698266No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTATTGTCAGCAACATTATACTACT6900.13022135743497898No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6870.12965517762004428No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC6840.12908899780510957No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6790.12814536478021843No Hit
GTCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC6700.12644682533541435No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACTATCAACTGTAAGTCCAGTCAGAATGTTTTATCCAGGTCCAA6690.1262580987304361No Hit
GTGTATTACTGTGCGAAAGTTCGGCCGGACGGACTAGCTGCCCATTGCGCCTCTGACAACTGGGGCCAGGGAACC6670.12588064552047967No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA6380.12040757397611097No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6380.12040757397611097No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTAGTCCCTTGGCCGAACGTC6310.11908648774126337No Hit
CTATCAACTGTAAGTCCAGTCAGAATGTTTTATCCAGGTCCAACAATAAG6250.117954128111394No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACTATCAACTGTAAGTCCAGTCAGAATGTTTTATC6250.117954128111394No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG6100.11512322903672054No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACTATCAACTGTAAGTCCAGT6040.11399086940685114No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5970.11266978317200353No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5960.1124810565670253No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT5950.11229232996204709No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC5930.11191487675209061No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT5800.10946143088737363No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC5790.1092727042823954No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC5770.10889525107243893No Hit
GTAGTATAATGTTGCTGACAATAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT5760.10870652446746071No Hit
CCTCAGACGTTCGGCCAAGGGACTAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG5630.1062530786027437No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC5560.10493199236789609No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG5480.10342217952807026No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5480.10342217952807026No Hit
GTTTATTATTGTCAGCAACATTATACTACTCCTCAGACGTTCGGCCAAGGGACTAAGGTGGAAATCAAACGAA5430.10247854650317909No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT5430.10247854650317909No Hit
AACCAGGACAGCCTCCTAAGTTGCTCATATACTGGGCATCTGTCCGGGAA5420.10228981989820088No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA5360.10115746026833149No Hit
CTGTAAGTCCAGTCAGAATGTTTTATCCAGGTCCAACAATAAGAACTACT5350.10096873366335325No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5320.10040255384841856No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5300.10002510063846211No Hit
GCCTGAAGATGTGGCAGTTTATTATTGTCAGCAACATTATACTACTCCTC5300.10002510063846211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)