FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences529867
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTATACTACTCCTCAGACGTTCGGCCAAGGGACTAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT25190.47540231794016236No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT18590.3508427586545303No Hit
GTATAATGTTGCTGACAATAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC17560.3314039183417725No Hit
GTATATGAGCAACTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTA14760.27856046894786807No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13920.2627074341296967No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13340.25176129104095935No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13160.24836421215135118No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11370.21458214986024796No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10960.20684435905614051No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10080.19023641781805622No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9910.18702806553342632No Hit
GGACTAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC9380.1770255554695801No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9120.17211866374014612No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGTTGGTACCAAGCTAAGTAGTTCTTATTGTTGGACCTGGATAAAACATT9080.1713637573202332No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8730.16475832614599514No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8520.16079506744145228No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG8330.1572092619468659No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8160.154000909662236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8100.1528685500323666No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8060.1521136436124537No Hit
CTCATATACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGACCGATTCAG7990.15079255737760608No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7850.14815038490791085No Hit
GTTTTATCCAGGTCCAACAATAAGAACTACTTAGCTTGGTACCAACAGAA7830.14777293169795438No Hit
TTATACTACTCCTCAGACGTTCGGCCAAGGGACTAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT7820.14758420509297618No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7700.1453194858332374No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7690.14513075922825916No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7480.14116750052371635No Hit
CCTTAGTCCCTTGGCCGAACGTCTGAGGAGTAGTATAATGTTGCTGACAATAATAAACTGCCACATCTTCAGG7460.14079004731375988No Hit
GTAATACACAGCCGTGTCGTCAGTTTTCAGGCTGTTCATTTGTAGAAACA7300.1377704216341082No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7040.13286352990467418No Hit
ATACTACTCCTCAGACGTTCGGCCAAGGGACTAAGGTGGAAATCAAACGA6990.13191989687978303No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTAGTCCCTTGGCCGAACGTC6970.1315424436698266No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC6600.12455955928563206No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGCCTGAAGATGTGGCAGTTTATTATTGTCAGCAACATTATACTACT6600.12455955928563206No Hit
GTGTATTACTGTGCGAAAGTTCGGCCGGACGGACTAGCTGCCCATTGCGCCTCTGACAACTGGGGCCAGGGAACC6450.12172866021095861No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA6230.11757667490143753No Hit
CTCCTAAGTTGCTCATATACTGGGCATCTGTCCGGGAATCCGGGGTCCCT6180.11663304187654638No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG6160.11625558866658992No Hit
GTAGTATAATGTTGCTGACAATAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT6090.1149345024317423No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5970.11266978317200353No Hit
GCTCATATACTGGGCATCTGTCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT5970.11266978317200353No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC5950.11229232996204709No Hit
GAGTAGTATAATGTTGCTGACAATAATAAACTGCCACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAAT5930.11191487675209061No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5900.11134869693715593No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5840.11021633730728655No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACTATCAACTGTAAGTCCAGTCAGAATGTTTTATC5830.11002761070230832No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5830.11002761070230832No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGAC5770.10889525107243893No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACTATCAACTGTAAGTCCAGTCAGAATGTTTTATCCAGGTCCAA5760.10870652446746071No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG5730.108140344652526No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA5690.1073854382326131No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT5690.1073854382326131No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5340.10078000705837503No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5340.10078000705837503No Hit
GTCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC5320.10040255384841856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)