FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences634754
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64471.0156690623454125No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG9320.14682853514904987No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC8560.13485539279783978No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7740.12193700236627103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7570.1192587994719214No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA7520.11847109273828917No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT7330.11547780715048664No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7180.11311468694958993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT352.5730422E-548.25779336
CGTTGTT400.002135228135.8997546
AGTCCGG400.002296343435.3715443
GTATCAA54600.031.9544091
TCTATCG554.2675983E-429.94755431
GGTATCA25900.029.1701831
GTCCGGA500.00667525528.44333544
TATCGCC606.673896E-427.74437133
CGCAGAG63950.027.134388
CGGTACC2650.026.97022845
TTAACGG2900.026.67384735
TACTCGA608.4042904E-426.6712285
AACGCAG66150.026.6612346
AGAGTAC64250.026.56340211
GCAGAGT64600.026.5144379
CAGAGTA64550.026.43786210
AGTGGTG3300.026.1862985
ATCAACG67050.026.156033
AACGGCC3000.026.04996537
TCAACGC67450.026.0009174
Produced by FastQC (version 0.11.5)