FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801295
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT17230.21502692516488933No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16670.20803823810207228No Hit
CTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACAC14250.1778371261520414No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13250.16535732782558232No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATCAACAGCTGGTTAGCCTGGTATCAGCAGAAA12210.15237833756606495No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12080.15075596378362524No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11920.14875919605139182No Hit
CGCTAAGAGATTCCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT11340.14152091302204556No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11280.14077212512245801No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGACCAC10970.1369033876412557No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10910.1361545997416682No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10470.1306634884780262No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10030.1251723772143842No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC9990.12467318528132584No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9880.12330040746541535No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9860.12305081149888616No Hit
CACTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACACGCTAAGAGATTCCCGGTCACCTT9790.12217722561603404No Hit
GGATATCAACAGCTGGTTAGCCTGGTATCAGCAGAAACCAGGGACAGCCCCTAAGCTCCTAATCTATGGTGCATC9720.12130363973318192No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9610.11993086191727143No Hit
CTCTTAGCGTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTAATAGTGAGAGTGAAATCTGTC9450.11793409418503796No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9370.11693571031892123No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9240.11531333653648157No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC9190.11468934662015862No Hit
AATCTATGGTGCATCCAGTTTGCAAGCTGGAGTCCCATCTCGGTTCAGCGGCAGTGGGTCTGGGACAGATTTCA8770.10944783132304581No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8450.10545429585857892No Hit
GTCTGGGACAGATTTCACTCTCACTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACACG8370.10445591199246221No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8340.10408151804266842No Hit
GTAATAAACAGCCGTGTCCTCGGGAGTCACAGAATTCAGCTGCAGGGAGAACTGGTTCTTCGATGTGTCTGCG8160.1018351543439058No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8150.10171035636064121No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA8100.10108636644431826No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8040.10033757854473072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA252.6479177E-454.85292438
ATCGCGT252.669518E-454.76278737
CGATCTA200.00767511451.20096235
TCGTATG1750.051.12581640
CGTATGC2000.050.01442741
GGCGAAT2500.048.97388532
AATCGCG352.403966E-548.8182136
GCGAATC2350.047.80325333
ATCTCGT1750.044.98371537
TAAGGCG2750.044.3767929
ATGCCGT2250.043.20495244
CGAATCT2200.041.83368334
ACGAGAC3050.041.00522222
CGAATCG504.1730164E-640.9040534
TATGCCG2500.040.18559343
GACTAAG3050.039.94684226
GTATGCC2600.039.8831142
CACGAGA3150.039.6960421
CTCTTAT1200.039.425361
GCGTATG350.001364659639.32755740
Produced by FastQC (version 0.11.5)