FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences801295
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27270.34032410036253813No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT16950.2115325816334808No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13620.16997485320637218No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12620.15749505487991314No Hit
CTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACAC12420.15499909521462132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11220.14002333722287047No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10880.1357802057918744No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10290.12841712477926356No Hit
CGCTAAGAGATTCCCGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT10250.1279179328462052No Hit
CTCTTAGCGTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCTGCTAATAGTGAGAGTGAAATCTGTC10140.12654515503029473No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGACCAC10010.12492278124785502No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10000.12479798326459045No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT9790.12217722561603404No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9690.12092924578338814No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATCAACAGCTGGTTAGCCTGGTATCAGCAGAAA9660.12055485183359436No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9170.11443975065362944No Hit
GCTAATAGTGAGAGTGAAATCTGTCCCAGACCCACTGCCGCTGAACCGAG8920.11131980107201467No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8900.11107020510548549No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8780.10957262930631041No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8470.1057038918251081No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC8290.10345752812634548No Hit
CACTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACACGCTAAGAGATTCCCGGTCACCTT8170.1019599523271704No Hit
GTCTGGGACAGATTTCACTCTCACTATTAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTATTGTCAACACG8120.10133596241084744No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8120.10133596241084744No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8040.10033757854473072No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)