FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145610
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTAGTTTGCAGAAATA35790.3124099824547621No Hit
GTGTACTACTGTGCGAGAGACTTCTGTAGTGGTGATAGATGCTCGTTAGG26620.2323652901074537No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT25720.2245092134321453No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA23650.2064402370789361No Hit
GACCTAGGACGGTGACCTTGGTCCCAGGACCGAAGACAAAAGTGCTGGTGCTTGTATATGAGCTGCAGTAATAAT21640.18889499917074745No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG21640.18889499917074745No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC21380.1866254659089917No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20790.1814753711996229No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20520.1791185481970304No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG19820.17300826633845726No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18900.16497761018147536No Hit
GATTATTACTGCAGCTCATATACAAGCACCAGCACTTTTGTCTTCGGTCCTGGGACCAAGGTCACCGTCCTAGGT18410.16070041288047415No Hit
GTACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA18400.1606131231396374No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT18150.1584308796187184No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC18100.1579944309145346No Hit
ACCTAGGACGGTGACCTTGGTCCCAGGACCGAAGACAAAAGTGCTGGTGC17720.15467742076273777No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17310.15109854138843062No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17000.14839255942249108No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGGACCGAAGACAAAAGTGCTGGTGCTTGTATATGAGCTG15680.13687031363203883No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT15370.13416433166609928No Hit
ACTATGGAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCC14650.12787947032585262No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14600.1274430216216688No Hit
GTATATGGTCTGATGGAAGTAATGACAACTATGGAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTT14270.12456246017405574No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14090.12299124483899407No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13930.12159460898560594No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13650.11915049624217665No Hit
CTCCTGTGCAGCGTCTGCATTTATCTTCAGTACTTATGGCATACACTGGGTCCGCCAGCCTCCAGGCAAGGGG13550.11827759883380906No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC13440.1173174116846047No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13350.11653180401707387No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG13140.11469871945950193No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13110.11443685023699166No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13050.1139131117919711No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC12830.11199273749356237No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12710.11094526060352126No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12290.10727909148837737No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12250.10692993252503033No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG12180.106318904339173No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATAACCTTGTCTCCTGGTACCAACAACA12080.10544600693080541No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12010.10483497874494811No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11780.10282731470570264No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC11520.10055778144394689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG100.008191933138.1595270
CGTGCCG350.004317778659.21121670
TCGTATG950.043.43707340
TTCCGTC350.001269958939.90852445
ATCGCGT350.00143244838.94299337
TCTGGAG13400.038.14852570
AGGGGGG2400.037.418270
GGTATCA7500.037.389491
AACGGGG750.001880071436.84253770
GTATCAA17050.036.026741
TCTCGTA1051.8189894E-1135.7998338
ACGAGAC2900.035.69980622
CGAGACT2950.035.09779423
TATACAC3450.033.8658143
CTTATAC3450.031.9320181
GTGGTAT2950.031.686011
TACACAT4100.030.9394285
CACGAGA3400.030.4418621
CCCACGA3400.030.43521519
ATCCCGT450.00542948529.67426
Produced by FastQC (version 0.11.5)