FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145610
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTACACAGCCGTGTCCTCGGCTCTCAGGCTGTTAGTTTGCAGAAATA32540.2840408166828153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28760.2510452946465202No Hit
GTGTACTACTGTGCGAGAGACTTCTGTAGTGGTGATAGATGCTCGTTAGG23940.2089716395632021No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23790.20766229345065076No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22650.19771126299526018No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC21980.19186285035919728No Hit
GACCTAGGACGGTGACCTTGGTCCCAGGACCGAAGACAAAAGTGCTGGTGCTTGTATATGAGCTGCAGTAATAAT21080.18400677368388893No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG20980.18313387627552136No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC20480.17876938923368338No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG19640.17143705100339557No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19560.1707387330767015No Hit
GATTATTACTGCAGCTCATATACAAGCACCAGCACTTTTGTCTTCGGTCCTGGGACCAAGGTCACCGTCCTAGGT18070.15773256169202432No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC17590.15354265413185988No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTT16860.14717050305077645No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC16860.14717050305077645No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC16740.14612302616073533No Hit
GTACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA15250.13311685477605817No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGGACCGAAGACAAAAGTGCTGGTGCTTGTATATGAGCTG15150.13224395736769057No Hit
ACCTAGGACGGTGACCTTGGTCCCAGGACCGAAGACAAAAGTGCTGGTGC15030.13119648047764948No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT14610.1275303113625056No Hit
GTATATGGTCTGATGGAAGTAATGACAACTATGGAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTT14280.1246497499148925No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14150.12351498328401463No Hit
CTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATAACCTTGTCTCCTGGTACCAACAACACCCAGGCAAAGC14030.12246750639397352No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13810.12054713209556482No Hit
ACTATGGAGAGTCCGTGAAGGGCCGATTCACCATCTCCAGAGACACTTCC13430.11723012194376796No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC13400.11696825272125767No Hit
CTCCTGTGCAGCGTCTGCATTTATCTTCAGTACTTATGGCATACACTGGGTCCGCCAGCCTCCAGGCAAGGGG13400.11696825272125767No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13120.1145241399778284No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12860.11225460671607265No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG12850.1121673169752359No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12720.11103255034435804No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12500.10911217604594932No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACGTTGGTGCTTATAACCTTGTCTCCTGGTACCAACAACA12410.10832656837841848No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12300.10736638122921414No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC11940.10422395055909078No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11810.10308918392821292No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT11760.10265273522402911No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG11720.10230357626068208No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11720.10230357626068208No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11480.10020862248059986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)