FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences976244
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53390.546891965533207No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGATTGCCACATGCTTGCTGCCTGCATATGAGTTG37150.38054011087392087No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30640.31385596223894846No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25910.265404960235351No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23860.24440611158685738No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG23100.23662117257570855No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21920.22453403042681955No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21490.22012939388103792No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT21390.2191050598006236No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT20540.21039822011710185No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19200.19667214343954995No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19190.1965697100315085No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18980.19441860846263842No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC18860.19318940756614125No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17870.18304850017003946No Hit
GATTATTACTGCAACTCATATGCAGGCAGCAAGCATGTGGCAATCGGCGGAGGGACCAAGCTGACCGTCCTGGGT17770.18202416608962513No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17490.17915603066446503No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17460.17884873044034072No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAACTCATAT17390.1781316965840507No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17280.17700492909559495No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17140.1755708613830149No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16980.173931926854352No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16410.16809322259599035No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16120.1651226537627888No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15940.16327885241804302No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTTCCAACAACACCCAGGCAAAGC15860.16245938515371156No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15660.16041071699288292No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15650.1603082835848415No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15310.1568255477114328No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15270.15641581407926708No Hit
CCCCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGAACCAGGAGA15260.15631338067122563No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTTTGTCTCCTGGTTCCAACAACA14340.14688950713141385No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG14110.14453353874646094No Hit
GTAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAAATACA14070.14412380511429518No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGATTGCCACATGCTTGCTGCCTGCATATGAGTTGCAGTAATAAT14040.1438165048901709No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG13860.14197270354542513No Hit
ACCCAGGACGGTCAGCTTGGTCCCTCCGCCGATTGCCACATGCTTGCTGC13740.14074350264892793No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13610.1394118683443893No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13220.13541696543077347No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC13160.13480236498252485No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12440.12742715960354173No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12390.12691499256333458No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA12310.12609552529900314No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12250.12548092485075452No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC12070.12363712350600875No Hit
GGATGAGGCTGATTATTACTGCAACTCATATGCAGGCAGCAAGCATGTGGCAATCGGCGGAGGGACCAAGCTGAC11910.12199818897734582No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11840.12128115512105579No Hit
GTTATAACCACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGTGACTG11810.12097385489693151No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11740.12025682104064148No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT11540.11820815287981283No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11470.1174911190235228No Hit
GTCCAGGAGACCCGGACGCGGAGGGAGGCTGAGTCAGGGCAGACTGGGCC11440.11718381879939851No Hit
GTGCAGGAGATGGTGACTGACTGTCCAGGAGACCCGGACGCGGAGGGAGG11380.11656921835114993No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11380.11656921835114993No Hit
GGTTATAACTTTGTCTCCTGGTTCCAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAG11350.11626191812702562No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC11350.11626191812702562No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC11270.11544245086269415No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCGTCTCTGGGCTCCAGG11240.11513515063856987No Hit
CTCCCTGACCGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACT11230.11503271723052845No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11090.11359864951794839No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT11090.11359864951794839No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG11050.11318891588578264No Hit
CCTGAGGGCCGCTTACTGACCTCATAAATCATGAGTTTGGGGGCTTTGCC11010.11277918225361694No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10990.11257431543753406No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10880.1114475479490783No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG10870.11134511454103688No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10720.1098086134204154No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10580.10837454570783533No Hit
GTCTATTACTGTGCGAGAGACATGGGCTGGTACCGATGGGACTATTGGGGCCAGGGAACCCTGGTCACCGTCTC10530.10786237866762817No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT10520.10775994525958675No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC10460.10714534481133814No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA10400.10653074436308957No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT10220.10468694301834376No Hit
GCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCC10060.10304800848968086No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA10020.10263827485751513No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9940.10181880759318367No Hit
GTTTGGGGGCTTTGCCTGGGTGTTGTTGGAACCAGGAGACAAAGTTATAA9840.10079447351276935No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC9830.1006920401047279No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA9810.10048717328864507No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA9800.10038473988060362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)