FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360979
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT98380.7228619986054157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC79790.5862691488994319No Hit
GTTATATTCAGCTCCAATAAGAGCTACTTAGCTTGGTACCAGCAGAAACC71770.5273409802796369No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC64000.47024972464674325No Hit
GTGTTATATTCAGCTCCAATAAGAGCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTC61450.45151321218034957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59110.43431970662295305No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGCTCTTATTGGAGCTGAATATAACACTCTGG55430.40728034745576525No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT54700.4019165615340134No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG54000.39677320517068965No Hit
CTCCTAAGTTGCTCCTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT51750.38024098828857755No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG51110.3755384910421101No Hit
ATCTCCACCTTGGTCCCCTGGCCAAAAGTGAACGGACTGCTATGAAACTG50250.3692195103671695No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50200.36885212776978926No Hit
GTTTCATAGCAGTCCGTTCACTTTTGGCCAGGGGACCAAGGTGGAGATCGCTCGAACTGTGGCTGCACCAT48660.357536743770477No Hit
GTGCAGCCACAGTTCGAGCGATCTCCACCTTGGTCCCCTGGCCAAAAGTG47020.3454865945764042No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT45410.3336568749407596No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC44880.3297626194085287No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC44530.3271909412268668No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42200.31007091218894633No Hit
GTTTATTACTGTCAGCAGTTTCATAGCAGTCCGTTCACTTTTGGCCAGGGGACCAAGGTGGAGATCGCTCGAA42080.3091891939552337No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTATATT40840.30007810554020303No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40550.2979472864753975No Hit
AACCAGGACAGCCTCCTAAGTTGCTCCTTTACTGGGCATCTACTCGGGAA39960.29361217182631033No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39850.29280393011207373No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG39400.28949748673565134No Hit
GAATATAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA38500.2828845999828065No Hit
GTAATAAACTGCCACATCTTCAGCCTCCAATCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA38050.2795781566063841No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37830.27796167317791093No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGT37420.2749491358793927No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG36940.27142226294454214No Hit
GCTATGAAACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTCCAATCTGCTGATGGTGAGAGTGAAAT36900.27112835686663794No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG36800.2703935916718774No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35890.26370722839955646No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG35330.2595925433088975No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGAT35260.2590782076725651No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT35090.2578291068414722No Hit
GGTGGAGATCGCTCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC31910.23446357364808715No Hit
GATTTCACTCTCACCATCAGCAGATTGGAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTTTCATAGCAGT31100.22851197557052677No Hit
GCCACAGTTCGAGCGATCTCCACCTTGGTCCCCTGGCCAAAAGTGAACGG30540.2243972904798678No Hit
GTCAGGGACCCCGGATTCCCGAGTAGATGCCCAGTAAAGGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA30010.22050303494763696No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA29710.21829873936335537No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29650.21785788024649902No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA29190.21447796035060054No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA29030.2133023360389837No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA29020.21322885951950762No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCGCTCGAACTGTGGCTGCACCA28980.21293495344160343No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28740.21117151697417816No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC28710.21095108741575No Hit
GGCCAAAAGTGAACGGACTGCTATGAAACTGCTGACAGTAATAAACTGCC28070.20624859016928257No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTA27330.2008113277280546No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27150.19948875037748565No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGCTCTTATTGGAGCTGAAT27150.19948875037748565No Hit
GTAAAGGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA26710.19625578352053924No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCCTT26690.19610883048158714No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25680.18868770201450574No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25500.18736512466393676No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGAGCGATCTCCACCTTGGTCC25170.18494039952122698No Hit
CCTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA25030.18391172824856225No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGATTGGAGGCTGAAGATG24130.17729884149571742No Hit
CTATGAAACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTCCAATCTGCTGATGGTGAGAGTGAAATCTGTC23720.1742863041971992No Hit
ATATAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA23610.17347806248296263No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23600.17340458596348657No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA23270.1709798608207768No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC23250.1708329077818247No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA23160.17017161910654022No Hit
GTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAA22900.16826122960016282No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22890.16818775308068676No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22840.1678203704833065No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22790.16745298788592625No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT21890.16084010113308142No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGATTGGAGGCTGAAGAT21720.1595910003019885No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21680.15929709422408428No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC21370.1570193221203266No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT21370.1570193221203266No Hit
CCTTGGTCCCCTGGCCAAAAGTGAACGGACTGCTATGAAACTGCTGACAGTAATAAACTGCCACATCTTCAGCCT21240.15606412736713793No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG21150.15540283869185345No Hit
CAGTAATAAACTGCCACATCTTCAGCCTCCAATCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA20670.15187596575700285No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19840.1457774146404904No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT19800.14548350856258618No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAGT19650.1443813607704454No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19580.143867025134113No Hit
GAGCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGC19530.14349964253673275No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19190.14100144087454694No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC19140.14063405827716666No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19070.14011972264083428No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18700.1374010914202203No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18640.13696023230336396No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA18640.13696023230336396No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC18580.13651937318650767No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18560.13637242014755555No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18540.13622546710860345No Hit
GGATCTCTGGTGTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG18260.13416812456327393No Hit
GGACCAAGGTGGAGATCGCTCGAACTGTGGCTGCACCATCTGTCTTCATC18100.13299250025165707No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17960.13196382897899234No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGAGTAGATGCCCAGTAAAG17710.130126915992091No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC17520.12873086212204596No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17320.12726133173252488No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17220.12652656653776437No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT17160.12608570742090802No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17060.1253509422261475No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT17030.12513051266771932No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG16870.12395488835610247No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG16840.12373445879767433No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC16550.12160363973286878No Hit
TTATTACTGTCAGCAGTTTCATAGCAGTCCGTTCACTTTTGGCCAGGGGA16540.1215301632133927No Hit
CACCATCAGCAGATTGGAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC16510.12130973365496456No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA16450.12086887453810823No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16270.11954629718753927No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGTCTACGGGGACATCGTGATGA16230.11925239110963505No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTATATTCAGCTCCAATAAGAGCTACTTAGCTTGGTACCAGCAGAAA15830.11631333033059289No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC15820.11623985381111684No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG15710.11543161209688026No Hit
GTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCCTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCTGA15520.11403555822683525No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT15130.11116997396726915No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA15050.11058216181146072No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14960.10992087313617624No Hit
GCAGAAACCAGGACAGCCTCCTAAGTTGCTCCTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCTGACCGATT14860.1091861079414157No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14850.10911263142193967No Hit
GCCTCCTAAGTTGCTCCTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT14690.1079370071103228No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14650.10764310103241859No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14600.1072757184350383No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14460.10624704716237356No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCCTTTACTGGGCATCTACTCGGGAATCCGGGGTC14150.1039692750586159No Hit
CTACTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGG14030.10308755682490325No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA14000.1028671272664751No Hit
AAGGTGGAGATCGCTCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT13850.10176497947433427No Hit
GCTCCTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT13850.10176497947433427No Hit
CTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGC13800.10139759687695402No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC13750.10103021427957375No Hit
CCCGAGTAGATGCCCAGTAAAGGAGCAACTTAGGAGGCTGTCCTGGTTTC13730.10088326124062164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9250.045.7468381
GTATCAA22700.038.4853321
GTGGTAT2650.037.345071
TCGCTAA501.9347985E-434.27427331
TAGATTG400.002739173434.1256685
TATCAAC26500.033.2242362
ATCAACG28500.030.8927083
TGGTATC3100.030.8231832
CGACCGG2000.030.70971311
TCAACGC28850.030.3996374
TCCTATA450.00487636630.333932
CAACGCA29250.030.1005885
AATGGTA5150.029.97272970
AACGCAG29400.029.9470146
CGTATGC703.79165E-529.63937241
TATACAC1401.6370905E-1129.250573
TGCACCG2250.028.8172286
CACCGAC2150.028.5661268
ACTCGCT854.7229296E-628.17803629
GCACCGA2200.027.9168977
Produced by FastQC (version 0.11.5)