FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1360979
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT97640.7174247361641877No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70370.5170542675529894No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC63180.4642246500497068No Hit
GTGTTATATTCAGCTCCAATAAGAGCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTC62060.4559952798683889No Hit
GTTATATTCAGCTCCAATAAGAGCTACTTAGCTTGGTACCAGCAGAAACC61600.4526153599724904No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60770.44651680885597794No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT56760.41705272454608044No Hit
CTCCTAAGTTGCTCCTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCT52100.3828126664702394No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48900.3593001802379023No Hit
ATCTCCACCTTGGTCCCCTGGCCAAAAGTGAACGGACTGCTATGAAACTG48900.3593001802379023No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC47810.35129123961501246No Hit
GTTTCATAGCAGTCCGTTCACTTTTGGCCAGGGGACCAAGGTGGAGATCGCTCGAACTGTGGCTGCACCAT47740.35077690397868005No Hit
ACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGCTCTTATTGGAGCTGAATATAACACTCTGG47660.3501890918228716No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG47480.34886651447230266No Hit
GTGCAGCCACAGTTCGAGCGATCTCCACCTTGGTCCCCTGGCCAAAAGTG47470.3487930379528266No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT43900.3225619204998754No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT43790.3217536787856389No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC42240.31036481826685053No Hit
GTTTATTACTGTCAGCAGTTTCATAGCAGTCCGTTCACTTTTGGCCAGGGGACCAAGGTGGAGATCGCTCGAA41900.30786661660466474No Hit
GTAATAAACTGCCACATCTTCAGCCTCCAATCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA40670.2988290047091101No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40520.2977268569169693No Hit
GGTGGAGATCGCTCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC40320.29625732652744824No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT39620.29111397016412455No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGT39050.28692580855398947No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38340.28170897567118963No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37910.2785494853337193No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTATATT37590.2761982367104856No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG36230.26620543006174235No Hit
GCTATGAAACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTCCAATCTGCTGATGGTGAGAGTGAAAT35690.26223769801003544No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT35430.26032730850365804No Hit
AACCAGGACAGCCTCCTAAGTTGCTCCTTTACTGGGCATCTACTCGGGAA34820.2558452408156187No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34520.2536409452313372No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34440.25305313307552874No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33880.24893844798486972No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33640.24717501151744442No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGAGT32740.2405621247645996No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGAT32130.23608005707656032No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31270.22976107640161975No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT31060.22821806949262258No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA30420.22351557224615515No Hit
GATTTCACTCTCACCATCAGCAGATTGGAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTTTCATAGCAGT30140.22145822970082565No Hit
GAATATAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA29950.22006217583078067No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29860.21940088715549613No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCGCTCGAACTGTGGCTGCACCA29080.21366971863636397No Hit
GCCACAGTTCGAGCGATCTCCACCTTGGTCCCCTGGCCAAAAGTGAACGG28140.20676292580561495No Hit
GTCAGGGACCCCGGATTCCCGAGTAGATGCCCAGTAAAGGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTA27980.2055873014939981No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA27780.20411777110447699No Hit
GGCCAAAAGTGAACGGACTGCTATGAAACTGCTGACAGTAATAAACTGCC27560.20250128767600384No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG27480.2019134755201954No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA26770.19669664263739559No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC26630.19566797136473082No Hit
GTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGCTCTTATTGGAGCTGAAT25720.18898160809240996No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA25550.18773250726131704No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGATTGGAGGCTGAAGATG25160.18486692300175095No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG24880.18280958045642143No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24470.17979704315790324No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA24210.17788665365152584No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT24020.17649059978148085No Hit
CTATGAAACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTCCAATCTGCTGATGGTGAGAGTGAAATCTGTC23660.17384544508034291No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23570.17318415640505844No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTA23450.17230243817134577No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23430.17215548513239365No Hit
CCTTTACTGGGCATCTACTCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCA23190.17039204866496838No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23170.17024509562601628No Hit
GTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAA22760.16723255832749806No Hit
CAGTAATAAACTGCCACATCTTCAGCCTCCAATCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA22410.1646608801458362No Hit
ACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGCTCCTT22300.1638526384315996No Hit
GTAAAGGAGCAACTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA22270.16363220887317145No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGATTGGAGGCTGAAGAT22180.16297092019788698No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC22070.16216267848365037No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21910.16098705417203352No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT21820.16032576549674904No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT21600.15870928206827586No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21220.15591717432818583No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20670.15187596575700285No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGAGCGATCTCCACCTTGGTCC20610.15143510664014653No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20560.15106772404276628No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20340.14945124061429307No Hit
CCTTGGTCCCCTGGCCAAAAGTGAACGGACTGCTATGAAACTGCTGACAGTAATAAACTGCCACATCTTCAGCCT20200.14842256934162834No Hit
ATATAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA19340.14210358866668774No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18910.13894409832921742No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18880.13872366877078926No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18790.13806238009550478No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18670.13718066186179215No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG18560.13637242014755555No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18520.13607851406965132No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18500.13593156103069923No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA18430.13541722539436685No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18140.1332864063295613No Hit
GAGCTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGTTGC17990.1321842585374205No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17920.13166992290108812No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA17260.1268204726156686No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17170.1261591839403841No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17020.1250570361482433No Hit
GGATCTCTGGTGTCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG17010.12498355962876724No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC16870.12395488835610247No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16620.12211797536920113No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16550.12160363973286878No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGTCTACGGGGACATCGTGATGA16530.12145668669391665No Hit
GGACCAAGGTGGAGATCGCTCGAACTGTGGCTGCACCATCTGTCTTCATC16430.12072192149915613No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16240.1193258676291111No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16000.11756243116168581No Hit
CACCATCAGCAGATTGGAGGCTGAAGATGTGGCAGTTTATTACTGTCAGC15510.11396208170735918No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15490.11381512866840708No Hit
TTATTACTGTCAGCAGTTTCATAGCAGTCCGTTCACTTTTGGCCAGGGGA15400.1131538399931226No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGAGTAGATGCCCAGTAAAG15330.11263950435679022No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG15180.11153735656464943No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15030.11043520877250862No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14940.10977392009722414No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14820.10889220186351149No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG14760.10845134274665516No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC14480.10639400020132567No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14130.10382232201966378No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13970.10264669770804692No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13910.10220583859119062No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG13830.10161802643538217No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTATATTCAGCTCCAATAAGAGCTACTTAGCTTGGTACCAGCAGAAA13700.10066283168219349No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13700.10066283168219349No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA13690.10058935516271741No Hit
GCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGAGCGATCTCCACCTTGGTCCCCTGGC13610.10000154300690899No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGA200.007667179751.2163326
GGTATCA9300.044.3229831
GTATCAA23850.038.9942741
ACCTTAG350.001434352738.933061
GTGGTAT2850.037.0547071
TGCACCG1950.034.9399266
TATCAAC27300.033.561122
AATGGTA4950.033.19901370
TGGTATC3100.032.9614522
ATCAACG27800.032.7100563
TCAACGC28150.032.4243474
CAACGCA28450.032.2045175
GACAGGT1800.032.1738471
ATTAGGT1108.1672624E-1032.13263357
AACGCAG28650.032.1044856
TATTAGG1108.3491614E-1032.0666156
CGAACTA553.4398225E-431.07339529
GGTAATC1051.7053026E-830.53751261
AGAGTAC30450.030.446111
TTATTAG1051.9073923E-830.1882855
Produced by FastQC (version 0.11.5)