FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893796
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37990.42504106082372267No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCATATTATAATGGTCTGCATATGAGGAG28780.3219974132799878No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28710.32121423680571404No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCATATTATAATGGTCTGCATATGAGGAGCAGTAATAAT27950.3127111779421703No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26280.2940268249130674No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23830.2666156483134854No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21170.23685494229108206No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20540.22980635402261815No Hit
GATTATTACTGCTCCTCATATGCAGACCATTATAATATGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT20410.22835188342753826No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGATGAGGGAGG18540.2074298833290818No Hit
CTCTTGGACAGTCAGTCACCTTCTCCTGCACTGGAACCAACAGTGATATT18270.20440905978545437No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17990.2012763538883593No Hit
GCATATGAGGAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGA17600.19691294210311974No Hit
GTGTCCGGGTCTCTTGGACAGTCAGTCACCTTCTCCTGCACTGGAACCAA17540.19624164798231364No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC17470.19545847150803985No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16380.18326329498006255No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16350.18292764791965954No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16060.17968305966909676No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15560.17408894199571265No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC15230.17039682433127917No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14830.16592153019257191No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14740.16491458901136277No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG14470.16189376546773537No Hit
CTCCTGCACTGGAACCAACAGTGATATTGGTGATTACGACTATGTCTCCTGGTTCCAACAACACCCAGGCAAAGC14270.15965611839838173No Hit
GTCAGTCACCTTCTCCTGCACTGGAACCAACAGTGATATTGGTGATTACGACTATGTCTCCTGGTTCCAACAACA14080.1575303536824958No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCATATTATAATGGT13720.15350258895765925No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13480.1508174124744349No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13450.15048176541403183No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13420.1501461183536288No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12850.14376882420597095No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12790.14309753008516485No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12660.141643059490085No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12510.13996482418806977No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCACCTTCTCCTG12430.1390697653603283No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12330.13795094182565149No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCAC12090.13526576534242712No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11980.13403505945428262No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11670.1305667064967845No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC11280.12620329471154493No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11160.12486070646993273No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11120.12441317705606202No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10940.12239929469364375No Hit
CTATTTACACTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGG10890.12183988292630533No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10870.12161611821936996No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT10780.12060917703816083No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGT10610.11870717702921026No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10340.11568635348558284No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCAC10320.11546258877864748No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCACCTTCT10090.11288929464889079No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC10090.11288929464889079No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10060.11255364758848777No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10030.11221800052808471No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10020.11210611817461702No Hit
CCTCATATGCAGACCATTATAATATGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG10000.11188235346768166No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG9970.11154670640727861No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAACGAGGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGAACCAGGAGA9830.10998035345873107No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT9740.10897341227752194No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9640.10785458874284512No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGA9540.1067357652081683No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9460.10584070638042685No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9200.10293176519026713No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9080.10158917694865495No Hit
GCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCACCTTCTCCTGCA9060.10136541224171959No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9050.1012535298882519No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9050.1012535298882519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGC750.054.7313428
TAGCGCT700.053.76604529
TCGTATG653.6379788E-1253.11428540
TGCGAGC200.007251464351.9420842
CGTATGC652.0918378E-1047.8922241
ATGCCGT751.6370905E-1146.27045444
GGTATCA9400.045.429611
CGCTCAT851.8189894E-1244.3325732
ACTAGCG851.8189894E-1244.24568627
GTAAGAC400.001610923738.0220768
ATCTCCG1001.0913936E-1137.5772710
GTATCAA22100.037.5639151
GTATGCC1051.6370905E-1136.2770142
CGAGACT1051.8189894E-1135.79188523
ACGAGAC1051.8189894E-1135.78988322
ATCTCGT905.3605618E-934.38430437
TATACAC1550.033.0610353
TGGTATC2600.031.535142
TTATACA1550.030.8569642
ATCAACG26950.030.5502763
Produced by FastQC (version 0.11.5)