FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893796
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC36190.4049022371995399No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCATATTATAATGGTCTGCATATGAGGAG27220.30454376613902945No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25030.2800415307296072No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCATATTATAATGGTCTGCATATGAGGAGCAGTAATAAT24710.2764612954186414No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24250.27131470715912803No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23360.2613571777005044No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22470.25139964824188066No Hit
GATTATTACTGCTCCTCATATGCAGACCATTATAATATGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT19670.22007258927092982No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19000.21257647158859516No Hit
GTGTCCGGGTCTCTTGGACAGTCAGTCACCTTCTCCTGCACTGGAACCAA18090.20239517742303612No Hit
CTCTTGGACAGTCAGTCACCTTCTCCTGCACTGGAACCAACAGTGATATT17220.19266141267134781No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16890.18896929500691434No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16850.1885217655930436No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16590.18561282440288387No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16560.18527717734248084No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16480.18438211851473937No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGATGAGGGAGG15740.17610282435813093No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15490.1733057655214389No Hit
GCATATGAGGAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGA15010.16793541255499017No Hit
CTCCTGCACTGGAACCAACAGTGATATTGGTGATTACGACTATGTCTCCTGGTTCCAACAACACCCAGGCAAAGC14570.16301258900241217No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14240.1593204713379787No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG13900.1555164713200775No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13860.1550689419062068No Hit
GTCAGTCACCTTCTCCTGCACTGGAACCAACAGTGATATTGGTGATTACGACTATGTCTCCTGGTTCCAACAACA13730.1536144713111269No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT13480.1508174124744349No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC13050.14600647127532457No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12960.14499953009411543No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCAC12920.1445520006802447No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12640.14141929478314963No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCACCTTCTCCTG12520.14007670654153745No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12500.13985294183460206No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11890.1330281182730735No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11800.13202117709186437No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC11660.13045482414331683No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11650.13034294178984915No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11560.12933600060864No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCATATTATAATGGT11530.12900035354823697No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11450.1281052947204955No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10870.12161611821936996No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10560.11814776526187185No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC10530.1178121182014688No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10460.11702894172719502No Hit
CCTCATATGCAGACCATTATAATATGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTG10380.11613388289945356No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10360.1159101181925182No Hit
CCCCTGAGGGCCGCTTAGTGACCTCATAAACGAGGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGAACCAGGAGA10320.11546258877864748No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10300.11523882407171213No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10240.11456752995090602No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT10220.11434376524397066No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT10060.11255364758848777No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCAC10000.11188235346768166No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGA9910.11087541228647253No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC9890.11065164757953717No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9640.10785458874284512No Hit
CTATTTACACTGGGTGCGACAGGCCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGTGG9630.10774270638937743No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9590.10729517697550671No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCACCTTCT9590.10729517697550671No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGATGGATCAACCCTAACAGTGGT9520.10651200050123294No Hit
GCCTCGCTCAGTGTCCGGGTCTCTTGGACAGTCAGTCACCTTCTCCTGCA9090.10170105930212264No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9070.10147729459518727No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9050.1012535298882519No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)