FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917890
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACTCTTCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT42530.4633452810249595No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA33310.3628975149527721No Hit
CTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC29990.3267276035254769No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT23780.25907243787381934No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22390.24392901110154813No Hit
CTCCTAAGCTGCTCATCTACTGGGCATCTACCCGGGAATCCGGGGTCCCT20460.22290252644652409No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA20220.2202878340541895No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18710.20383706108575103No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16970.18488054124132522No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTTGGAGCTGTATAAAACACT16900.1841179226268943No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16800.18302846746342155No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT16000.17431282615563956No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15200.16559718484785757No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14570.15873361731797928No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14030.15285055943522644No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13590.14805695671594638No Hit
GAGTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT13370.1456601553563063No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13330.14522437329091722No Hit
ATAGTACTCTTCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA13200.14380808157840264No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12940.14097549815337349No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12310.1341119306234952No Hit
GTTTATTACTGTCAGCAGTATTATAGTACTCTTCGCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA12290.13389403959080062No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12190.1328045844273279No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11520.12550523483206047No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10930.11907744936757128No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10790.11755221213870944No Hit
GTACTATAATACTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT10690.1164627569752367No Hit
AACCAGGACAGCCTCCTAAGCTGCTCATCTACTGGGCATCTACCCGGGAA10160.11068864460883113No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10040.10938129841266382No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC9920.10807395221649654No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA9750.10622187843859286No Hit
GTAGATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA9480.10328034949721646No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC9460.10306245846452189No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9450.1029535129481746No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA9190.10012092952314548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGG200.00841609750.02157214
GCGATAA200.00841609750.02157214
GTGGTAT3000.046.6893461
GGTATCA12300.043.652641
GCCGTCT3350.041.76573646
TCGTATG3500.041.2463140
TGGTATC3400.041.196482
CCGTCTT3500.040.09950347
ACCGAGG3800.039.60722727
ATCGTAT350.00139118239.17442339
AGACCGA3850.039.0373925
ATATCGT350.001432635838.94111337
AACCGTA350.001590817838.1199810
GACCGAG3950.038.06571626
ACGAGAC3950.038.0159222
ATCTCGT2700.037.85941337
CGTATGC3650.037.81010441
GAGACCG4000.037.5611924
AGGTACG451.2226909E-437.0550353
CGAGCCC4100.034.97633715
Produced by FastQC (version 0.11.5)