FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998420
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29460.29506620460327315No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23120.23156587408104806No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20430.20462330482161817No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20050.20081729132028606No Hit
GTCTATTACTGTGCGACCGTTCGTGGGTTCAACTCTGGCTACTTAGCCTC19840.19871396806954988No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGCG18700.18729592756555358No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA18250.18278880631397607No Hit
TTATTACTGTCAGCAATATGGCAGCTCACCACTCACTTTTGGCGGAGGGA18170.18198754031369563No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17210.17237234831033033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16840.16866649305903328No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16200.16225636505678973No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16120.1614550990565093No Hit
CAGTAATAAAGTGCAAAATCTTCAGGCTCCAGTCTGCTGATGCTGAGAGTGAAGTCTGTCCCAGACCCAGAACCA14310.14332645580016426No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14180.14202439854970855No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14130.14152360729953328No Hit
GTCTGGGACAGACTTCACTCTCAGCATCAGCAGACTGGAGCCTGAAGATT14050.14072234129925282No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13540.135614270547465No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13400.1342120550469742No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13160.1318082570461329No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12990.13010556679553695No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12950.12970493379539674No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12880.12900382604515134No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGATTGTTGCCAG12840.12860319304501114No Hit
GTGCAGCCACAGTTCGTTTGACGTCCACCTTGGTCCCTCCGCCAAAAGTG12810.12830271829490594No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12670.12690050279441517No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12430.12449670479357386No Hit
CTCCTGCAGGGCCAGTCAGATTGTTGCCAGCAGTTATGTAGCCTGGTACC12380.12399591354339858No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC12030.12049037479217163No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACGTCCACCTTGGTCCCTCCGCCAAAAGTGAGTGGTGAG11970.11988942529196131No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11950.11968910879189118No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC11900.1191883175417159No Hit
GAGTGAAGTCTGTCCCAGACCCAGAACCACTGAATCTGTCCGGGATGCCA11800.11818673504136537No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11760.11778610204122514No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC11630.11648404479076942No Hit
GTCCCAGACCCAGAACCACTGAATCTGTCCGGGATGCCAGCGGCCCTGCT11590.11608341179062918No Hit
GACTTCACTCTCAGCATCAGCAGACTGGAGCCTGAAGATTTTGCACTTTATTACTGTCAGCAATATGGCAGCTCA11500.1151819875403137No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11380.11398008853989303No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10990.11007391678852588No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT10610.10626790328719375No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA10590.10606758678712365No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10330.10346347228621222No Hit
GTTATGTAGCCTGGTACCAACAAAAACCTGGCCAGGCGCCCAGACTCCTCATCTATGGTGGAGGCAGCAGGGCCG10140.10156046553554615No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10080.10095951603533583No Hit
CTTTATTACTGTCAGCAATATGGCAGCTCACCACTCACTTTTGGCGGAGGGACCAAGGTGGACGTCAAACGAA10070.10085935778530078No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10060.10075919953526573No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10050.10065904128523068No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10000.1001582500350554No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)