FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857238
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGACACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAACATCAC31230.3643095616386581No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC24180.2820686903753683No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19590.22852463376565202No Hit
CCCTATGATTACCACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCA17950.20939342399660305No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17170.2002944339844944No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT15500.18081326306113354No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC14970.1746306160016238No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14700.17148096561281698No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG14660.17101435074040114No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14620.1705477358679853No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATCATAGGGTGTTCGGCG14620.1705477358679853No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAACATCACCTGCTTTGGAAATGC14350.16739808547917848No Hit
CCTATGAGCTGACACAGACACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAACATCACCTGCTTTGGAAATG14260.16634820201624287No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT14180.1654149722714112No Hit
ATCATAGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCC14080.16424843509037163No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG13840.16144874585587665No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13570.15829909546706983No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13510.1575991731584461No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13390.15619932854119858No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGC13180.1537496004610155No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12790.14920010545496118No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12630.14733364596529785No Hit
GGCCAGGCCCCTGCGCTGGTCATCTATGAGGACAATAAACGACCCTCCGG12600.14698368481098598No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12280.14325076583165935No Hit
CCCCTGCGCTGGTCATCTATGAGGACAATAAACGACCCTCCGGGATCCCT12110.14126765262389207No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGACACCCTCGGTGTCAGTGTCCCCAGGACAAACGG12000.13998446172474854No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11980.13975115428854065No Hit
GCGCAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTT11930.13916788569802085No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11690.13636819646352588No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11650.13590158159111004No Hit
CCCCAGGACAAACGGCCAACATCACCTGCTTTGGAAATGCATTGCCAAAA11380.13275193120230322No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG11340.13228531632988738No Hit
GGTCATCTATGAGGACAATAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAAGGGC11310.1319353551755755No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATAGGGTGTTCGG11240.1311187791488478No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11100.1294856270953924No Hit
GACACAGACACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAACATCA10710.1249361320893381No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10560.12318632631777872No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAACATCACCTGCTTTGG10530.12283636516346687No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10460.12201978913673915No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10290.12003667592897188No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT10110.11793690900310065No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9970.11630375694964526No Hit
CAGTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGACAGTCAAGGT9950.11607044951343734No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC9660.11268749168842258No Hit
GTAGTAGTCAGCTTCATCCTCCACCTGGGCCCCACTGACAGTCAAGGTGG9430.11000445617203158No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTTGGCAATGCATTTCCAAAGCAGGTGATGTTGGC9130.10650484462891285No Hit
ATAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG9060.10568826860218516No Hit
CCTTGACTGTCAGTGGGGCCCAGGTGGAGGATGAAGCTGACTACTACTGT9020.10522165372976933No Hit
TAGATGACCAGCGCAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGC8960.10452173142114558No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8920.10405511654872975No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC8800.10265527193148227No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCT8790.10253861821337831No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8680.10125542731423479No Hit
TCTATGAGGACAATAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC8650.10090546615992291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGCA300.002206946770.13505670
ACCGGTC252.9394444E-453.7055668
GACGCGG200.006454029653.49460653
AGGGGGG2650.052.93211470
GTCGTAT200.007514971351.47503739
CAGCGGT306.5663765E-445.63040538
GGTATCA8550.041.6212461
ATGCCGT3250.036.3822544
CGTATGC3250.036.09644741
GTATCAA21150.035.7147371
GCCGTCT3350.035.4397346
ACTAGGC3400.034.63200827
TCGTATG3300.034.4255140
TGTCGTA400.002700681834.22280538
TATACAC3550.034.0446933
GTTTGCG400.002895860733.73917830
TCTCGTA2850.033.62240638
CGAGACT3400.033.57185423
ACGAGAC3400.033.56989322
CACGAGA3400.033.5659821
Produced by FastQC (version 0.11.5)