FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences857238
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35300.41178762490696863No Hit
GTCTCTGAGGCCTCCTATGAGCTGACACAGACACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAACATCAC27660.3226641842755454No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC22320.26037109880803233No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCT20040.2337740510803301No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19620.2288745949199639No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16850.19656151500516778No Hit
CCCTATGATTACCACTGCTGTCTGTTGAGTAACAGTAGTAGTCAGCTTCA16150.18839575473789075No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14930.174164001129208No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG14700.17148096561281698No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13630.15899901777569356No Hit
ATCATAGGGTGTTCGGCGGCGGGACCAAGCTGACCGTCCTAGGTCAGCCC13510.1575991731584461No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13410.1564326359774065No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAACATCACCTGCTTTGGAAATGC13200.1539829078972234No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGC13140.15328298558859968No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12950.15106656494462448No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12630.14733364596529785No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12580.14675037737477806No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12430.14500057160321872No Hit
CTACTACTGTTACTCAACAGACAGCAGTGGTAATCATAGGGTGTTCGGCG12300.14348407326786727No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11800.1376513873626694No Hit
GACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCTATGATTACCA11740.13695146505404565No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG11610.13543496671869423No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC11130.1298355882497043No Hit
GACTACTACTGTTACTCAACAGACAGCAGTGGTAATCATAGGGTGTTCGG11020.12855239735056076No Hit
CCTATGAGCTGACACAGACACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAACATCACCTGCTTTGGAAATG10850.12656928414279348No Hit
GGCCAGGCCCCTGCGCTGGTCATCTATGAGGACAATAAACGACCCTCCGG10710.1249361320893381No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG10670.12446951721692226No Hit
GCGCAGGGGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTT10670.12446951721692226No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG10660.1243528634988183No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10490.12236975029105103No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGACACCCTCGGTGTCAGTGTCCCCAGGACAAACGG10390.12120321311001145No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT10050.11723698669447692No Hit
CCCCTGCGCTGGTCATCTATGAGGACAATAAACGACCCTCCGGGATCCCT9950.11607044951343734No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9930.11583714207722942No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCGCCGCCGAACAC9880.11525387348670965No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC9790.11420399002377403No Hit
GGTCATCTATGAGGACAATAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAAGGGC9790.11420399002377403No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9750.11373737515135819No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC9280.1082546504004722No Hit
CCCCAGGACAAACGGCCAACATCACCTGCTTTGGAAATGCATTGCCAAAA9260.10802134296426431No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC9170.10697145950132869No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8970.10463838513924954No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC8910.10393846283062579No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAACATCACCTGCTTTGG8620.10055550500561106No Hit
GGCCTGGCCTGACTTCTGCTGGTACCAAGAAGCATATTTTTTTGGCAATGCATTTCCAAAGCAGGTGATGTTGGC8590.10020554385129918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9150.035.9511531
GTGGTAT3400.035.5411451
GTATCAA21450.035.5227361
TGGTATC3600.033.5411572
TATCAAC24450.031.414832
ATCAACG25200.030.4638583
TCAACGC25450.030.0258734
CAACGCA25500.029.9652525
TACCGCA450.005327859429.7882797
AACGCAG26200.029.1629526
CGCAGAG27850.027.5554968
TAACGGC500.00821908727.25783736
AGAGTAC28600.026.36572511
GCAGAGT29450.025.944639
CAGAGTA29200.025.82396510
CCTTATA650.001015794725.820491
GAGTTTA2600.025.820491
AGTACGG23200.024.12413413
GAGTACG23350.024.1126912
ACCGCCT700.001578982623.93840612
Produced by FastQC (version 0.11.5)