FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194368
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54230.4540476637016397No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49430.4138590451184225No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38270.3204205069124424No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36190.3030054388597149No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35210.2948002625656414No Hit
GTGTAAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACAATCA32070.26851020790912017No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG31860.2667519558461044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31760.26591469295895404No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30930.25896541099560605No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30540.2557000857357196No Hit
GAGTTACAGTTTCTCTCCGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT28360.2374477547958418No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28290.23686167077483655No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27940.23393125066981033No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACAATCACTT27720.23208927231807955No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26720.22371664344657594No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA26540.2222095702497053No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26210.21944660272210909No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25910.21693481406065804No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25380.21249732075876113No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG25260.21149260529418068No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24970.20906454292144463No Hit
GGCCAAAAGTGTACGGAGAGAAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTG24850.20805982745686422No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24260.2031199764226771No Hit
GTAATAAACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA24090.2016966295145215No Hit
GATCAGGAGTTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAGTTTAATT24030.20119427178223126No Hit
GTCTGCATCTGTGGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGA23850.1996871985853606No Hit
GATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGTATCTGGGACAGATTTCA23660.19809639909977492No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23660.19809639909977492No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG23580.19742658879005467No Hit
CTCCTGATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCATCAAGGTT23150.19382635837530812No Hit
CTGCTACTCTGGCTCCGAGGTGTAAGATGTGACATCCAGATGACCCAGTC22930.19198438002357734No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT22500.1883841496088308No Hit
ACTTACTACTGTCAACAGAGTTACAGTTTCTCTCCGTACACTTTTGGCCA22440.18788179187654055No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC22310.1867933501232451No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22090.1849513717715143No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGTAAGATGTGACAT21270.17808581609688134No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATACACTGCCA20150.16870847176079734No Hit
GTTTATTACTGTGTGAGAGATAGTGGAGTCTGTGTTGATAGCCACTGTCA20080.1681223877397921No Hit
GTGGGAGACAGAGTCACAATCACTTGCCGGGCAAGTCAGAGCGTCAACAGGCAATTAAACTGGTATCAGCAGAAA19710.16502451505733576No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTT19490.16318253670560498No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19450.16284763155074483No Hit
GCATAGATCAGGAGTTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAGTT19410.16251272639588468No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATT19110.1600009377344336No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18820.15757287536169756No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC18370.15380519236952095No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18240.15271675061622547No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18190.1522981191726503No Hit
GTTTAGGGGCTTTCCCGGGTTTCTGCTGATACCAGTTTAATTGCCTGTTG17630.1476094470046083No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17470.14626982638516772No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17350.1452651109205873No Hit
GTGTATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAA17130.1434231325688565No Hit
GAGTGAAATCTGTCCCAGATACACTGCCACTGAACCTTGATGGGACCCCA16930.14174860679455578No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16740.1401578073089701No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGTAAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC16130.1350505036973529No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16120.13496677740863786No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16000.13396206194405744No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTTTCTCTCCGTACA15950.13354343050048226No Hit
ACCTTGATGGGACCCCACTTTGCAAACTGGAAGCAGCATAGATCAGGAGTTTAGGGGCTTTCCCGGGTTTCTG15650.13103164183903118No Hit
CAATTAAACTGGTATCAGCAGAAACCCGGGAAAGCCCCTAAACTCCTGAT15470.12952456864216053No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15340.12843612688886508No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14920.12491962276283355No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14730.12332882327724787No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGT14590.12215665523523737No Hit
CCTTGGTCCCCTGGCCAAAAGTGTACGGAGAGAAACTGTAACTCTGTTGA14560.12190547636909228No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14490.12131939234808702No Hit
GAGAGAAACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGT14450.12098448719322688No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14440.12090076090451184No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14260.1193936877076412No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14210.11897505626406603No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14110.11813779337691566No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT14020.11738425677848034No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13880.11621208873646985No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13880.11621208873646985No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13870.11612836244775479No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13790.1154585521380345No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13790.1154585521380345No Hit
GTCCCAGATACACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACT13780.11537482584931948No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13760.1152073732718894No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13720.11487246811702925No Hit
CATCAAGGTTCAGTGGCAGTGTATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG13640.11420265780730898No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13580.11370030007501876No Hit
ATATTACACAGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACG13460.11269558461043831No Hit
GTTCAGTGGCAGTGTATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA13370.111942048012003No Hit
CCCCTAAACTCCTGATCTATGCTGCTTCCAGTTTGCAAAGTGGGGTCCCA13350.11177459543457292No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13300.11135596399099776No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13250.11093733254742258No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC13230.1107698799699925No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGATACACTGCCACTGAACCT13160.11018379594898724No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12960.10850927017468652No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12960.10850927017468652No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12860.10767200728753618No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12760.1068347444003858No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC12640.10583002893580538No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12630.10574630264709034No Hit
GTGTAATATATGGGAGAACCACTACCACTAATGTATGCAAGCCACTCCAGCCCCCTCCCTGGAGCCTGGCGGACC12310.1030670614082092No Hit
GTCTCCCACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTGGATGT12310.1030670614082092No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGTATCTGGGACAGATTTCACT12130.10155998821133855No Hit
GTTTAATTGCCTGTTGACGCTCTGACTTGCCCGGCAAGTGATTGTGACTC12030.1007227253241882No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12000.10047154645804307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)