FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483239
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGCAGAGACA50520.34060593066929873No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42530.2867373363294789No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG38700.260915469455698No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35600.24001526389206326No Hit
GACTTACAGTATCCCTCAGTCGTTCGGCCAGGGGACCAGGTTGGAAATCAAACGAACTGTGGCTGCACCAT34060.2296325811281931No Hit
CTGTAAGTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC31200.2103504559952914No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30870.2081255954030335No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA30760.2073839752056142No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA30300.2042826543800426No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28250.1904615507008648No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG27480.1852702093189297No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26820.18082048813441395No Hit
CAGTAATACACGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGCAGAGA26490.17859562754215605No Hit
ACCTACTACTGTCAACAGACTTACAGTATCCCTCAGTCGTTCGGCCAGGGGACCAGGTTGGAAATCAAACGAA26400.17798884738063117No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26350.17765174729089514No Hit
GTGTATTACTGTGCGAGACCAGGATATGGTGGCTACAATTTAGACTCCTGGGGCCAGGGAACCCTGGTCACCGT25830.17414590635764027No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCAATGTAAATTGGTATCAGCAGAAA25800.17394364630379863No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCA24260.1635609635399285No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT23180.15627960160162993No Hit
GTCTCCATCCGCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG23120.1558750814939467No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23060.15547056138626342No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22680.1529086007042695No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21880.1475149992684928No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21100.14225623786861052No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG20520.1383458768276724No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAACCTGGTCCCCTGGCCGAACGAC20330.13706489648667547No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC20210.13625585627130896No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG20030.1350422959482592No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20010.13490745591236475No Hit
CTCCTGTGCAGCCTCCGGATTCACCTTTAGTAACTATTGGATGACCTGGGTCCGCCAGACTCCAGAGAAGGGG19830.133693895589315No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19750.13315453544573733No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19730.13301969540984293No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19340.13039031470990178No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19250.1297835345483769No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18750.12641253365101646No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18510.12479445322028344No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18470.12452477314849461No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAACCTGGTCCCCTGGCCGAACGACTGAGGGATA18170.12250217261007834No Hit
CTATTGGATGACCTGGGTCCGCCAGACTCCAGAGAAGGGGCTGGAGTGGG17900.12068183212550371No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC17880.1205469920896093No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC17480.11785019137172095No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17450.11764793131787933No Hit
CTACTACTGTCAACAGACTTACAGTATCCCTCAGTCGTTCGGCCAGGGGA16930.11414209038462446No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16810.11333305016925795No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT16730.1127936900256803No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC16700.11259142997183866No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTTTTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT16610.11198464981031378No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT16480.11110818957700005No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT16440.11083850950521124No Hit
GGATATGGTGGCTACAATTTAGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGGGAGTGCATCCGCC16260.10962494918216148No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16070.1083439688411645No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15970.10766976866169242No Hit
ACCATATCCTGGTCTCGCACAGTAATACACGGCCGTGTCGTCGGCTCCCA15820.1066584683924843No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGACTTACAGTATC15560.10490554792585685No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15380.1036919876028071No Hit
GTATTACTGTGCGAGACCAGGATATGGTGGCTACAATTTAGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTC15310.10322004747717664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)