FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483239
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGCAGAGACA39680.2675226312145244No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39240.2645561504248472No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG37330.25167892699693034No Hit
GACTTACAGTATCCCTCAGTCGTTCGGCCAGGGGACCAGGTTGGAAATCAAACGAACTGTGGCTGCACCAT31790.21432823705417672No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA29360.19794517269300496No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA29210.19693387242379684No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28740.19376513158027803No Hit
CTGTAAGTCTGTTGACAGTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC28330.19100091084444248No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC27800.1874276498932404No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26620.17947208777546975No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26400.17798884738063117No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26210.1767078670396342No Hit
GTGTATTACTGTGCGAGACCAGGATATGGTGGCTACAATTTAGACTCCTGGGGCCAGGGAACCCTGGTCACCGT25830.17414590635764027No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24660.16625776425781685No Hit
ACCTACTACTGTCAACAGACTTACAGTATCCCTCAGTCGTTCGGCCAGGGGACCAGGTTGGAAATCAAACGAA24520.16531388400655592No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24000.16180804307330107No Hit
GTAGTAGGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCA23460.1581673621041518No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23350.1574257419067325No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCAATGTAAATTGGTATCAGCAGAAA23030.1552683013324218No Hit
CAGTAATACACGGCCGTGTCGTCGGCTCCCAGGCTGTTCATTTGCAGAGA21690.14623401892749585No Hit
GTCTCCATCCGCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG21250.14326753813781865No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20750.1398965372404582No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20370.1373345765584643No Hit
CTCCTGTGCAGCCTCCGGATTCACCTTTAGTAACTATTGGATGACCTGGGTCCGCCAGACTCCAGAGAAGGGG20120.13564907610978408No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20080.13537939603799523No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19350.130457734727849No Hit
GTGCAGCCACAGTTCGTTTGATTTCCAACCTGGTCCCCTGGCCGAACGAC18710.1261428535792276No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18620.12553607341770273No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC18610.12546865339975555No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18450.1243899331126002No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17750.11967053185629559No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17500.11798503140761536No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC17380.11717599119224886No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17280.11650179101277676No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG17230.11616469092304073No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17230.11616469092304073No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT16800.11326563015131076No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16590.11184980977441937No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16580.11178238975647216No Hit
CTATTGGATGACCTGGGTCCGCCAGACTCCAGAGAAGGGGCTGGAGTGGG16440.11083850950521124No Hit
GAGTTGGGGCTGAGCTGGGTTTTCCTTGTTGCTTTTTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAGT16140.10881590896679497No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCAACCTGGTCCCCTGGCCGAACGACTGAGGGATA15930.10740008858990359No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15850.10686072844632592No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15690.10578200815917058No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT15680.10571458814122335No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15670.10564716812327617No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACCTACTACTGTCAACAGACTTACAGTATC15370.1036245675848599No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15340.10342230753101828No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15320.10328746749512384No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15010.10119744693876037No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC14910.10052324675928828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTC301.0148795E-556.4796458
AATCGTT252.5819702E-455.1368639
TAATCGT252.6039078E-455.04251538
CCTAACG252.7959587E-454.2552072
GTGGTAT2950.040.2451971
CTAACGC350.001470233338.738043
CCGGTCT350.001471939638.7288979
GTATCAA26250.035.407011
TCCGAGA10250.035.1541570
CTGCCGT400.002835284233.88664212
TATCAAC28550.032.306082
CGGTCTG450.00504703330.12146210
ATCAACG30550.030.0679453
TCAACGC30900.029.6106854
CAACGCA31300.029.1240085
AACGCAG31550.029.0006436
CTCGCTA605.9732626E-428.27890823
GTGAGCG7250.028.26117970
TACTCCG606.021787E-428.2398225
CGAACCT500.006940007728.22051453
Produced by FastQC (version 0.11.5)