FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257048
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT54690.43506691868568265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47510.37794897251338055No Hit
GTCTGGGACCGACTACTCCCTCACCATCAGCAGACTGGAGCCTGAAGATT40830.3248085991943028No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37090.2950563542521845No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACTA35170.2797824744958029No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC35080.2790665113822225No Hit
CATCTATGGTGCGTCCATCAGGGCCACCGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACCGACTACTC34290.27278194627412794No Hit
GTATGGTAACTCACCTCACACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT32780.26076967625739034No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32090.25528062571994065No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCGGTGGCCCTGAT31490.2505075382960714No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31290.2489165091547817No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTAGTTTTTGTCAACACTCTGA30550.2430297013320096No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29790.23698379059510855No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29770.23682468768097958No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29370.23364262939840008No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGC27400.217970992356696No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACAAAAACTACTTAGCCTGGTTCC27160.2160617573871483No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTG25660.20412903882747516No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24270.1930713862955114No Hit
GTATTTCTGTCACCAGTATGGTAACTCACCTCACACGTTCGGCCAAGGGA24240.19283273192431793No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24180.192355423181931No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23550.18734368138686827No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23410.18622996098796546No Hit
TCTATGGTGCGTCCATCAGGGCCACCGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACCGACTACTCC22770.18113866773583823No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22590.17970674150867746No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCT22440.17851346965271017No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22060.17549051428425963No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGC21470.17079697831745486No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21040.1673762656636819No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT20980.16689895692129497No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20370.16204631804036124No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19600.15592085584639567No Hit
GTTACCATACTGGTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGAGTAGTCGGT19460.15480713544749286No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19460.15480713544749286No Hit
GTGTATTTCTGTCACCAGTATGGTAACTCACCTCACACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA18890.15027270239481708No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17460.13889684403459535No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAA17240.1371467119791766No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17160.1365103003226607No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16880.13428285952485505No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16770.13340779349714568No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16710.13293048475475877No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16610.13213497018411388No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16530.13149855852759798No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16290.12958932355805028No Hit
GTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA15850.12608905944721283No Hit
CCTCACACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG15630.1243389273917941No Hit
CACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTAGTTTTTGTCAACACT15490.12322520699289127No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15350.12211148659398846No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15270.12147507493747256No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14770.11749750208424818No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14710.11702019334186124No Hit
CAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGAGTAGTCGGTCCCAGACCCACTGCCA14430.1147927525440556No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14290.11367903214515276No Hit
GAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGAGTAGTCGGTCCCAGACCCACTGCCA14280.11359948068808828No Hit
GTCTATTATTGTGCGAAGGAAGTAAAGTTCGGAATGTGGACTTTTGATAT14140.11248576028918547No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13880.11041742240550877No Hit
ATAATAGACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGATACA13820.10994011366312186No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13780.1096219078348639No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13540.1077126728653162No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13490.10731491557999376No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13370.10636029809521991No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13340.10612164372402644No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13310.105882989352833No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13280.10564433498163953No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT13270.10556478352457505No Hit
TCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACCGACTACTCCCTCACC13140.10453061458273671No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13140.10453061458273671No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12930.10286003398438245No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12810.10190541649960862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC200.006834117752.7277850
TTTAGCG200.00776949251.04464331
CGATATT200.00794042850.76441215
GGTATCA10250.049.5282251
CTTATAC3450.041.2017441
TATACAC3400.040.8106043
GTCACCG7250.040.48975470
ATCTCGT2700.039.35615537
TGTCGTA350.001377055839.25704238
TTATACA3650.038.9425622
ACGAGAC3600.038.5494822
TCGTATG2850.037.4978240
CCCTAAG3650.037.1798628
GTATCAA28250.036.899261
GTGGTAT2850.036.8130871
CGTATGC2950.036.2884741
TGGTATC2800.036.2602922
CATCTCG2950.035.97450636
ATGCCGT3000.034.7412944
GTCGTAT400.002628965334.41244539
Produced by FastQC (version 0.11.5)