FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257048
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53540.4259185011232665No Hit
GTCTGGGACCGACTACTCCCTCACCATCAGCAGACTGGAGCCTGAAGATT40560.3226607098535617No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38590.3069890728118576No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37100.29513590570924897No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36730.29219250179786294No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36530.29060147265657316No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAACTA34340.2731797035594504No Hit
GTATGGTAACTCACCTCACACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT31120.24756413438468539No Hit
CATCTATGGTGCGTCCATCAGGGCCACCGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACCGACTACTC30920.2459731052433956No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30570.24318880424613856No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTCGGTGGCCCTGAT29890.2377793051657534No Hit
GTGTTGACGCAGTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGC29100.23149474005765888No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28490.22664210117672515No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTAGTTTTTGTCAACACTCTGA28040.2230622856088232No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGACAAAAACTACTTAGCCTGGTTCC27820.22131215355340447No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27210.21645951467247077No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27150.21598220593008383No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTG25440.20237890677205642No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25140.19999236306012183No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24870.19784447371938066No Hit
GTATTTCTGTCACCAGTATGGTAACTCACCTCACACGTTCGGCCAAGGGA24530.19513972417918807No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24280.19315093775257589No Hit
TCTATGGTGCGTCCATCAGGGCCACCGACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACCGACTACTCC22540.17930898422335503No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCT22320.17755885216793632No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21980.17485410262774373No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21860.17389948514296988No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGC21570.17159249288809975No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21440.1705583239462614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21350.169842360832681No Hit
GTGTATTTCTGTCACCAGTATGGTAACTCACCTCACACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA20300.16148945784090982No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20260.16117125201265187No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20200.16069394327026496No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19790.15743233353062094No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19600.15592085584639567No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19430.1545684810762994No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18610.1480452615970114No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC18440.14669288682691511No Hit
GTTACCATACTGGTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGAGTAGTCGGT18120.1441472402008515No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT16890.13436241098191956No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16820.13380555078246814No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAAA16450.13086214687108208No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16340.12998708084337274No Hit
GTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA16220.1290324633585989No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16210.12895291190153438No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15950.12688457401785772No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15800.1256913021618904No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15030.11956583996792486No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14950.11892942831140894No Hit
CCTCACACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG14570.11590647294295843No Hit
CACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGAACCAGGCTAAGTAGTTTTTGTCAACACT14380.11439499525873315No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14270.1135199292310238No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14090.11208800300386301No Hit
GAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGAGTAGTCGGTCCCAGACCCACTGCCA13770.10954235637779942No Hit
GTCTATTATTGTGCGAAGGAAGTAAAGTTCGGAATGTGGACTTTTGATAT13710.10906504763541248No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13700.108985496178348No Hit
ATAATAGACAGCCGTGTCCTCAGTTCTCAGGCTGTTCATTTGCAGATACA13620.1083490845218321No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13600.10818998160770313No Hit
CAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGAGTAGTCGGTCCCAGACCCACTGCCA13250.10540568061044606No Hit
CTGCTACTCTGGCTCCCAGAAACTACCGGAGAGACTGTGTTGACGCAGTC13190.10492837186805914No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13030.10365554855502734No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12870.10238272524199553No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12860.10230317378493105No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12640.10055304172951231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG209.5284464E-567.4497155
GTGGTAT2800.045.7803761
GGTATCA10750.040.479491
GTATCAA26900.037.118741
TATCAAC29350.033.894522
GGGACGT502.1238999E-433.7342536
TGGTATC3500.033.722172
ATCAACG29500.033.7208333
TCAACGC29600.033.606914
CAACGCA30450.032.6726845
AACGCAG30500.032.5176056
ACGCAGA32400.030.7233897
CGCAGAG32650.030.2898778
AGAGTAC32800.030.06042311
GCAGAGT33650.029.1926089
TTCCGCA855.2133746E-627.82211519
AATGGTA7950.027.24930870
ATAGCTA1000.00848563827.07899770
GTATAGA500.00863195326.9863281
CTAGGTT500.00864704126.9766674
Produced by FastQC (version 0.11.5)