FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479314
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATG59900.40491741442317186No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTACTACTATCCCACAC52040.35178467857398765No Hit
GGATTACCTGTGGCGGATACAACATTGGAAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC50070.3384676951614059No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48980.33109941499911444No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44700.30216708555452054No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT44250.2991251350288039No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC40370.2728967616070692No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTA38980.2635005144276334No Hit
CCCCAGGACAGATGGCCAGGATTACCTGTGGCGGATACAACATTGGAAGT34680.23443298718189648No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTT33130.2239551575933169No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT32620.22050761366417138No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC30520.20631184454416035No Hit
GTTTTACTTCCAATGTTGTATCCGCCACAGGTAATCCTGGCCATCTGTCCTGGGGCCACTGACACCGAGGGTGG29840.20171511930529964No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC28850.195022828148723No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA28770.19448203694415114No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28440.19225127322529229No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT28080.18981771280471893No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27890.1885333336938608No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27820.18806014138986044No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG27750.18758694908586004No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26670.18028626782414012No Hit
GGATAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC26460.178866690912139No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26320.17792030630413827No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26040.1760275370881368No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT26030.17595993818756533No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC25810.17447276237499273No Hit
GGGTGGCCGTGTTCGCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGGCGGTCGCTATCATCATAGA25690.17366157556813497No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT25670.17352637776699198No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG25420.17183640525270497No Hit
GTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTA25300.1710252184458472No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATGGCCAGGATTAC25020.16913244922984574No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC24950.16865925692584535No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGCCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG24760.16737487781498722No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGCGACTGAAGGTGATTCCAGAGGCGGCACA24720.1671044822127013No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24430.16514411409612834No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24220.16372453718412724No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC24050.16257535587441205No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23890.16149377346526836No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23420.15831662513840877No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23080.15601826251897838No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC23030.15568026801612098No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGCCCCTCAGG22890.15473388340812025No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22640.15304391089383323No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA22610.1528411141921188No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGCTATTCGGCG22550.15243552078868988No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC22210.15013715816925954No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG21700.146689614240114No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA21360.14439125162068364No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21350.14432365272011216No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21200.14330966921153995No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG20980.14182249339896735No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20900.1412817021943955No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT20870.14107890549268107No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG20670.13972692748125143No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC20460.13830735056925034No Hit
TAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCC20310.13729336706067813No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19920.13465700993839036No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC19810.13391342203210407No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG19560.13222344951781703No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCA18670.1262071473669552No Hit
AAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCG18650.12607194956581225No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGATGGCCAGGATTACCTGTGGCGGATACAACATTGGAAGTAAAACTGTG18370.1241791803498108No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17920.12113722982409414No Hit
CCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGG17850.12066403752009378No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTTCCAATGTTGTATCCGCCACAGGTAATCCTGGC17750.11998804851437897No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17690.11958245511095007No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTC17520.11843327380123489No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17220.11640530678409047No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATGG17060.11532372437494677No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATGGCCAG16830.1137689496618027No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16710.11295776285494494No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16620.1123493727498016No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16580.11207897714751569No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTAC16390.11079459803665755No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16320.11032140573265715No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGGCGGTCGCTATCATCATAGACGACCACCACAGGGGC16210.10957781782637087No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16140.1091046255223705No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC16080.10869903211894161No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16080.10869903211894161No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15860.10721185630636902No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15610.10552188379208201No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15490.10471069698522423No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15450.1044403013829383No Hit
GCTTGGTCCCTCCGCCGAATAGCACATGATCACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC15270.10322352117265166No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15220.10288552666979424No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15200.10275032886865128No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15190.10268272996807981No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15160.10247993326636537No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15070.10187154316122203No Hit
GTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGGCGGTCGCTATCAT14830.10024916954750648No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14810.10011397174636352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGC200.007677545351.1990623
TATCGCT306.65165E-445.5133510
GGTATCA12700.044.3456421
GTGGTAT2800.043.8849071
ACTATCG350.001420283939.0114448
CTACGCT2750.038.5237528
ACTACGC2750.038.51332527
TACGCTG2900.037.72360629
GTATAAT557.9593065E-637.2356831
GTATCAA29550.036.7316471
ATAATAC754.4194167E-836.408223
AGGGGGG3200.035.4011470
ACGAGAC3000.035.27046222
TGGTATC3500.035.107932
ACGGGTT400.00273738834.13039816
TCGTATG1253.6379788E-1233.08500740
CGAGACT3200.033.06941623
TATACAC3200.033.0660633
ATCTCGT1253.6379788E-1232.98593537
GACTACG3250.032.58378626
Produced by FastQC (version 0.11.5)