FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479314
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATG52400.35421823899456095No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTACTACTATCCCACAC49520.33474975562997444No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG46860.31676844807796045No Hit
GGATTACCTGTGGCGGATACAACATTGGAAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC46560.31474048106081604No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC45310.3062906184893809No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39680.26823243746763703No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC36680.2479527672961927No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCACTA35060.23700174540361277No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33480.22632111911331876No Hit
GGGTGGCCGTGTTCGCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGGCGGTCGCTATCATCATAGA31420.21239574559559363No Hit
CCCCAGGACAGATGGCCAGGATTACCTGTGGCGGATACAACATTGGAAGT30220.20428387752701593No Hit
CCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTT29280.19792958087329668No Hit
GTTTTACTTCCAATGTTGTATCCGCCACAGGTAATCCTGGCCATCTGTCCTGGGGCCACTGACACCGAGGGTGG29110.19678039956358148No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACT29100.19671280066301No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29080.19657760286186707No Hit
CTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGAC28940.1956312182538663No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28830.19488763034758003No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28080.18981771280471893No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA27580.1864377677761449No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT26730.180691861227569No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26620.17994827332128271No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26070.17623033378985123No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATGGCCAGGATTAC26050.17609513598870827No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTC25690.17366157556813497No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGC25670.17352637776699198No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25240.1706196250424183No Hit
GGATAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC25230.17055202614184684No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC24590.16622569650527205No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG24510.1656849053007002No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24110.16298094927784096No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23820.161020581161268No Hit
GTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTA23690.16014179545383872No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23490.1587898174424091No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23460.15858702074069467No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23440.1584518229395517No Hit
CCCCTGTGGTGGTCGTCTATGATGATAGCGACCGCCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTG23350.15784343283440838No Hit
GGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCGCCCCTCAGG22680.15331430649611913No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGGCATAGCGACTGAAGGTGATTCCAGAGGCGGCACA22520.15223272408697544No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22480.15196232848468952No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21550.1456756307315418No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21440.14493204282525549No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCA21260.1437152626149688No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21070.14243088350411068No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCCGGGGATGAGGCCGACTAT20900.1412817021943955No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20770.14040291648696626No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC20710.13999732308353738No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA20580.13911853737610813No Hit
CTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGCTATTCGGCG20030.13540059784467665No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC19690.1331022352252463No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19680.1330346363246748No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG18800.12708593307438448No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18790.127018334173813No Hit
GGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACAGTTTTACTTCCAATGTTGTATCCGCCACAGGTAATCCTGGC18570.1255311583612404No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18410.12444957595209671No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACATGATCA18160.12275960343780967No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17980.12154282322752302No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC17860.12073163642066526No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG17800.12032604301723637No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC17560.11870366940352083No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17470.11809527929837749No Hit
AAGTAAAACTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGGTGGTCGTCTATGATGATAGCGACCG17450.11796008149723453No Hit
CCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGCGAACACGG17430.11782488369609158No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17160.11599971338066158No Hit
TAGTAGTAGTGATCATGTGCTATTCGGCGGAGGGACCAAGCTGACCGTCC17130.11579691667894713No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17100.11559411997723269No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17080.11545892217608973No Hit
GTCCTGGGGCCACTGACACCGAGGGTGGCTGAGTCAGCACATAGGAGGTC16980.11478293317037493No Hit
CCCTCGGTGTCAGTGGCCCCAGGACAGATGGCCAGGATTACCTGTGGCGGATACAACATTGGAAGTAAAACTGTG16880.1141069441646601No Hit
GTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATGGCCAG16850.11390414746294565No Hit
GTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGTGG16750.11322815845723085No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16600.11221417494865864No Hit
CAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGCTGATGGTCAGGGT16360.11059180133494309No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16230.10971301562751384No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGACAGATGG16200.1095102189257994No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16160.10923982332351347No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15480.10464309808465276No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15350.1037643123772235No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15200.10275032886865128No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15110.10214193876350797No Hit
GCTTGGTCCCTCCGCCGAATAGCACATGATCACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCCC15100.10207433986293647No Hit
GAGTTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGGCGGTCGCTATCATCATAGACGACCACCACAGGGGC14920.10085755965264982No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14800.10004637284579203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3000.046.6460341
TGGTATC3400.041.135992
GTATCAA26950.038.3739051
GGTATCA10800.038.2396351
GTCTTTA451.0671321E-437.9236031
TATCAAC29750.034.2847672
GTATAAA807.7998266E-834.1312451
ATCAACG32350.031.6176073
TCAACGC32950.030.9352574
CAACGCA33100.030.7950735
AACGCAG33750.030.302996
ACGCAGA35300.028.875837
TAACGCC605.814967E-428.4090524
AGAGTAC36100.028.13863611
CAGAGTA36900.027.6209610
TACGCAC500.007723753327.60866541
AATGGTA4600.027.183370
CTAATAC1209.555697E-825.5707423
CGGTTGG8950.023.83340370
GCGCAAT750.001778938923.45426856
Produced by FastQC (version 0.11.5)