FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609478
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAAGGAAGATGGAACTGAGAAATACTATGTGGACTCTGTGAAGGGGCGATTCACCATCTCCAGAGACAACG14680.24086185227358495No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14430.23675998149235902No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC11620.1906549539113799No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACACCCA11130.18261528718017714No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10770.17670859325521182No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10690.17539599460521954No Hit
CCCAAGCACTGAGGCTTCTGTCCCATGCTAAGCAGTAATAGTCAGCCTCG10130.16620780405527352No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9870.1619418584427986No Hit
CCTTTATACTGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC9540.1565273890115804No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT8780.14405770183665365No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8720.14307325284915945No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCACTAATGGTCAGGGAGG8280.13585396027420185No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8280.13585396027420185No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACACCCAAGCACTGAGG8230.13503358611795668No Hit
GACTATTACTGCTTAGCATGGGACAGAAGCCTCAGTGCTTGGGTGTTCGG8220.13486951128670763No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8120.1332287629742173No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC7910.1297831915179875No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG7640.12535317107426355No Hit
GTGCTTGGGTGTTCGGAGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC7620.1250250214117655No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC7550.12387649759302223No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT7430.1219075996180338No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG7250.11895425265555115No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAGTAACTAG7210.118297953330555No Hit
CACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAGCTTGGCTGCAGCAGCACCAGGGCCACCCTCC7080.11616498052431753No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC6870.11271940906808778No Hit
GTAAAACACGGCCGTGTCCTCGACTCTCAGACGGTTCACTTGCAGATACA6870.11271940906808778No Hit
CTATTACTGCTTAGCATGGGACAGAAGCCTCAGTGCTTGGGTGTTCGGAG6850.11239125940558971No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6800.11157088524934451No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG6760.11091458592434839No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT6710.11009421176810319No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6690.10976606210560513No Hit
CTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATT6630.10878161311811091No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6580.10796123896186571No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT6540.10730493963686959No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT6530.10714086480562056No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC6510.10681271514312249No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG6500.10664864031187343No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC6490.1064845654806244No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAGTTGGGGCTGAGCTGGG6410.10517196683063211No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG6400.10500789199938308No Hit
GGCTTGAGACAGACCGCCACACTCACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAGCTTGGCTG6390.10484381716813404No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT6300.10336714368689272No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6290.10320306885564369No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA6180.10139824571190428No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC6150.10090602121815717No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG6130.10057787155565912No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG6110.10024972189316102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1700.058.7263170
ATGCCGT1100.056.75610444
TCTCGTA1000.055.0216438
ATCTCGT1000.054.953437
ACGAGAC1650.053.6989622
CGTATGC1300.053.15626541
TCGTATG1200.051.73698440
GATCTCG1200.051.46774336
ACTGCGT200.007724052351.11727522
ACACATC1900.050.2287256
TGCCGTC1050.049.61941545
GGTATCA4550.048.6911131
CGAGACA1850.047.89759423
TCCGAGC1850.047.89366513
CGAGCCC1750.046.73579415
TATACAC1900.046.6371353
CTTGAAA1450.046.4173457
CACGAGA1850.046.051621
GACATCT2000.044.33073426
CTTATAC2000.044.308911
Produced by FastQC (version 0.11.5)