FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609478
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16190.2656371517921894No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG14680.24086185227358495No Hit
GTATAAAGGAAGATGGAACTGAGAAATACTATGTGGACTCTGTGAAGGGGCGATTCACCATCTCCAGAGACAACG12780.20968763433626808No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA10690.17539599460521954No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10490.17211449798023884No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC10320.16932522584900522No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACACCCA9450.15505071553033908No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG8900.1460265998116421No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC8640.14176065419916717No Hit
CCTTTATACTGGCCACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACC8590.14094028004292197No Hit
GACTATTACTGCTTAGCATGGGACAGAAGCCTCAGTGCTTGGGTGTTCGG8450.13864323240543547No Hit
GTGCTTGGGTGTTCGGAGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC8420.13815100791168836No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG8240.1351976609492057No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8100.1329006133117192No Hit
GTAATAGTCAGCCTCGTCCTCAGGCTGGAGTCCACTAATGGTCAGGGAGG7990.13109579016797981No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGGTCATCCAGTAACTAG7740.12699391938675392No Hit
CCCAAGCACTGAGGCTTCTGTCCCATGCTAAGCAGTAATAGTCAGCCTCG7730.1268298445555049No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG7540.1237124227617732No Hit
CTCCTATCCTACAGGAATAACAACCGGCCCTCAGGGATCTCAGAGAGATT7310.11993870164304536No Hit
GTAAAACACGGCCGTGTCCTCGACTCTCAGACGGTTCACTTGCAGATACA6870.11271940906808778No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC6870.11271940906808778No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT6820.11189903491184258No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT6790.11140681041809548No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC6660.10927383761185801No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT6610.10845346345561284No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA6610.10845346345561284No Hit
CTATTACTGCTTAGCATGGGACAGAAGCCTCAGTGCTTGGGTGTTCGGAG6560.10763308929936766No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTACTAGTTACTGGATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG6540.10730493963686959No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG6420.10533604166188114No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT6390.10484381716813404No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC6380.10467974233688501No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCTCCGAACACCCAAGCACTGAGG6320.10369529334939079No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG6240.1023826946993985No Hit
CACCTGCATTGGGAACAGCAACAATGTTGGCCACCAAGGAGCAGCTTGGCTGCAGCAGCACCAGGGCCACCCTCC6160.10107009604940621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)