FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237650
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG29510.23843574516220256No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28980.23415343594715793No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG26980.2179937785318951No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA25780.20829798408273742No Hit
GCATAATACTTTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT24810.20046055023633497No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24650.19916777764311397No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23610.1907647557871773No Hit
GTATTATGCTGTAGACAGTAATAAGTTGCGAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC23520.1900375712034905No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC20680.16709085767381732No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20050.16200056558800954No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGTAGATACA19940.16111178443017007No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19660.15884943239203328No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGACTGACTTTCAGTAACGCCTGG19630.15860703753080435No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18570.15004241910071506No Hit
ACTTATTACTGTCTACAGCATAATACTTTTCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17780.14365935442168626No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17400.14058901951278632No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17250.1393770452066416No Hit
GATCAGGACTGCACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA17230.139215448632489No Hit
GCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGCC17220.13913465034541267No Hit
GGACTGCACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGATTTTC16490.13323637538884175No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16380.13234759423100229No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16350.13210519936977336No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATTAGAGATGATTTAGGCTGGTATCAGCAGAAA15780.12749969700642347No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15720.12701490728396558No Hit
GTAGATACACCGTGTCTTTTGAATCATCTCTTGAGATGGTGAATCTGCCT15660.1265301175615077No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15270.12337898436553145No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15200.12281339635599725No Hit
CTCCTGTGCAGCCTCTGGACTGACTTTCAGTAACGCCTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG15130.12224780834646305No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15060.12168222033692887No Hit
GTAATAAGTTGCGAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13980.11295600533268696No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT13780.11134003959116068No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13540.10940088070132913No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13290.10738092352442127No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13170.10641134407950552No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13130.10608815093120026No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT13100.1058457560699713No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12770.10317941259645295No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT12720.10277542116107138No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12560.10148264856785036No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12500.10099785884539249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)