FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050322
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTACCTCACTCCCTTTCGGCGGAGGGACGAAGGTGGAGATCAAACGAACTGTGG51820.49337250862116566No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT44030.41920477720165816No Hit
GTATGGTACCTCACTCCCTTTCGGCGGAGGGACGAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT43740.41644371916421824No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC38400.3656021677161861No Hit
GTACCATACTGCTGACAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGCTGACGGTGAGAGTGAAGTCTGTC37540.357414202501709No Hit
CAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGCTGACGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA36240.3450370457821506No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCCGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31170.2967661345758729No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28960.27572496815262365No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28050.2670609584489328No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26810.255255055116431No Hit
GTCTGGGACAGACTTCACTCTCACCGTCAGCAGACTGGAGCCTGAAGATT24100.22945344380104388No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23950.22802531033340254No Hit
GTCTTTGTCTCCGGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCGACTTAGCCTGGTA23520.22393132772616395No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23130.22021818071029645No Hit
CATCTATGGTGCATCCCACAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA23000.2189804650383406No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22490.21412481124836003No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22140.21079249982386355No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT21850.2080314417864236No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCGGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG21240.20222369901801543No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20470.19489261388412316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19600.18660943977180333No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19560.1862286041804323No Hit
GTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCAGTGGCCCTGTG19380.18451484401926266No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19110.18194420377750822No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19010.18099211479908067No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCGTCAGCAGAC18340.17461311864361595No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18130.17261373178891806No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18100.1723281050953898No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTCGTCC17690.16842454028383677No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17330.16499701996149752No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17040.1622359619240576No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTCGTCCCTCCGCCGAAAGGG16680.15880844160171834No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16480.1569042636448632No Hit
GACTTCACTCTCACCGTCAGCAGACTGGAGCCTGAAGATTTTGCAATGTATTACTGTCAGCAGTATGGTACCTCA15640.14890671622607163No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT15470.14728816496274477No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCCGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC15170.14443189802746206No Hit
TCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC14770.1406235421137518No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14280.1359583061194567No Hit
GTCAGAGTGTTGGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG14270.13586309722161394No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14040.13367329257123053No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTC13720.1306266078402623No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13550.12900805657693545No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13450.12805596759850787No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCA13340.12700866972223757No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13210.12577095405028174No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13180.12548532735675344No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13150.12519970066322517No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13110.12481886507185415No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC12870.12253385152362799No Hit
ATGGTACCTCACTCCCTTTCGGCGGAGGGACGAAGGTGGAGATCAAACGA12720.12110571805598663No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCCGCGCAGCTTCTCT12630.12024883797540183No Hit
ATGTATTACTGTCAGCAGTATGGTACCTCACTCCCTTTCGGCGGAGGGAC12590.11986800238403081No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12550.11948716679265978No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTCTCCAGTCACC12480.11882070450776047No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11890.11320337953503781No Hit
CTGCAGGAGAGGGTGGCTCTCTCCCCCGGAGACAAAGACAGGGTGACTGG11880.11310817063719507No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11670.11110878378249717No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11480.1092998147234848No Hit
GTAATACATTGCAAAATCTTCAGGCTCCAGTCTGCTGACGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11240.10701480117525862No Hit
CCCAGATACCACCGGCGAAATTGTGTTGACGCAGTCTCCAGTCACCCTGTCTTTGTCTCCGGGGGAGAGAGCCAC11230.10691959227741588No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11020.10492020542271797No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10850.1033016541593911No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT10830.1031112363637056No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG10560.10054059612195118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)