FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050322
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTACCTCACTCCCTTTCGGCGGAGGGACGAAGGTGGAGATCAAACGAACTGTGG49950.47556844472457016No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45070.42910650257730487No Hit
GTATGGTACCTCACTCCCTTTCGGCGGAGGGACGAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT41200.392260659112158No Hit
GTACCATACTGCTGACAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGCTGACGGTGAGAGTGAAGTCTGTC35870.3415143165619686No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34120.32485275943948616No Hit
CAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGCTGACGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA34120.32485275943948616No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30650.29181527188804957No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29110.277153101620265No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27360.2604915444977826No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25840.24601979202568355No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25420.24202101831628775No Hit
GTCTGGGACAGACTTCACTCTCACCGTCAGCAGACTGGAGCCTGAAGATT24490.23316659081691138No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCCGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA24480.23307138191906862No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21960.20907873966269389No Hit
GTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCAGTGGCCCTGTG21620.2058416371360402No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21570.20536559264682638No Hit
CATCTATGGTGCATCCCACAGGGCCACTGGCATCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA21400.20374704138349953No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20870.19870096979783342No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20510.1952734494754942No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20490.19508303167980867No Hit
GTCTTTGTCTCCGGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCGACTTAGCCTGGTA19920.18965612450277153No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT19530.18594297748690403No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC18090.17223289619754703No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18040.17175685170833327No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17910.17051913603637742No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17570.16728203350972368No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17450.1661395267356106No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCGTCAGCAGAC17210.16385451318738445No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17190.16366409539169893No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTCGTCCCTCCGCCGAAAGGG17180.16356888649385617No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16300.15519050348369356No Hit
GTCTCCAGTCACCCTGTCTTTGTCTCCGGGGGAGAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG16220.15442883230095153No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTCGTCC15940.15176298316135434No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT15840.15081089418292676No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15690.14938276071528542No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15550.14804983614548684No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT14900.14186125778570763No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCGGTCTGGGATGCCA14490.13795769297415458No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14480.13786248407631183No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14410.13719602179141252No Hit
TCCCAGACCGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACC14180.13500621714102912No Hit
GACTTCACTCTCACCGTCAGCAGACTGGAGCCTGAAGATTTTGCAATGTATTACTGTCAGCAGTATGGTACCTCA13750.13091223453379058No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13470.12824638539419342No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13370.1272942964157658No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13030.12405719388911211No Hit
GTCAGAGTGTTGGCAGCGACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG12740.12129613585167215No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12440.11843986891638945No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCCGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC12150.11567881087894949No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11990.1141554685134654No Hit
ATGGTACCTCACTCCCTTTCGGCGGAGGGACGAAGGTGGAGATCAAACGA11900.1132985884328806No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTC11880.11310817063719507No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11830.11263212614798128No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11730.11168003716955373No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCCGCGCAGCTTCTCT11710.1114896193738682No Hit
GTAATACATTGCAAAATCTTCAGGCTCCAGTCTGCTGACGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11660.11101357488465442No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11500.1094902325191703No Hit
ATGTATTACTGTCAGCAGTATGGTACCTCACTCCCTTTCGGCGGAGGGAC11430.10882377023427102No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGCGAAATTGTGTTGACGCAGTCTCCAGTCACC11380.10834772574505724No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11210.10672917448173037No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10880.10358728085291939No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC10760.1024447740788063No Hit
CTGCAGGAGAGGGTGGCTCTCTCCCCCGGAGACAAAGACAGGGTGACTGG10640.10130226730469323No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10570.10063580501979393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)