FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398457
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46990.33601319168197524No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGTCGAGAGTGCTGGAGCTTGTATATGAG45710.3268602466861691No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39650.28352677272164967No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG36450.2606444102321344No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35690.2552098491408745No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34050.243482638364998No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC32860.23497325981420952No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT32430.23189844235468093No Hit
GATTATTACTGCAGCTCATATACAAGCTCCAGCACTCTCGACGTATTCGG32320.23111186114410381No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGTCGAGAGTGCTG31670.22646388126342107No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA31090.2223164530621964No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28510.20386754830502476No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT27420.19607324358203362No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26480.18935154960073852No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTTCCAACAGCACCCAGGCAAAGC26270.1878498945623641No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC25080.17934051601157563No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24970.17855393480099851No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24890.17798187573876065No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24670.17640871331760646No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTTCCAACAGCA24510.17526459519313073No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23810.17025907839854926No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23720.16961551195353164No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23180.16575411328342593No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGCGAGG23060.16489602469006912No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23020.1646099951589502No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23010.16453848777617044No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22780.16289381797223654No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA22330.15967598574714845No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22310.159532970981589No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22080.15788830117765507No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21980.15717322734985775No Hit
GCTTATAACTATGTCTCCTGGTTCCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAT21270.15209620317249653No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20800.148735356181849No Hit
GTGCTGGAGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC20240.1447309427461838No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20120.14387285415282702No Hit
CTCCAGCACTCTCGACGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT20100.14372983938726755No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19730.14108406622441733No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19380.1385813078271266No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC18780.1342908648603425No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18640.1332897615014262No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18220.13028645142467735No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGTCGAGAGTGCTGG18050.12907082591742186No Hit
GTGTATTACTGTGCGGCGAGGGTAGTTCGGAAAGACCAGCACTGGGGCCA17980.12857027423796372No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17250.12335023529504305No Hit
GCCCAGAGATGGTCAGCGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16970.12134802857721046No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC16710.11948883662493734No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16410.11734361514154527No Hit
GCCTGGAGCCCAGAGATGGTCAGCGAGGCCGTGTTGCCAGACTTGGAGCC16030.11462633459591534No Hit
GGTTCCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC15730.11248111311252329No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCTCCAGCACTCTCGA15720.11240960572974357No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15720.11240960572974357No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGTCGAGAGTG15510.11090795069136913No Hit
GGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAATCGGCCCTCAGG15410.11019287686357179No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15110.10804765538017973No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15080.10783313323184053No Hit
CCCCTGAGGGCCGATTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGAACCAGGAGA15080.10783313323184053No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC15030.10747559631794186No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14950.10690353725570395No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14720.10525886745177004No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA14690.10504434530343085No Hit
GAGATGGTCAGCGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT14480.10354269026505641No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC14350.10261309428891986No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG14340.10254158690614013No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14180.10139746878166436No Hit
GCTTGGTCCCTCCGCCGAATACGTCGAGAGTGCTGGAGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCT14170.10132596139888461No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14010.10018184327440886No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAA250.001070422584.167370
TCGTATG2700.047.20857640
GGTATCA15350.045.1756631
TAAGGCG4000.044.29157629
GCGAATC3200.043.7845833
TCTGGAG12100.043.47484670
ATCTCGT2850.043.3095537
TCTCGTA2800.042.9280838
CGTATGC3000.042.55402841
AATCTCG2900.042.5122336
GGCGAAT3700.042.44151732
CGAATCT3100.040.81833634
ACGAGAC4250.040.7953422
TATACAC4250.040.7909663
ATCTCCG4450.039.7172810
TCTCCGA4500.039.27597811
CGAGACT4350.039.08438523
CCCACGA4450.038.9548819
CGTCGTC459.335327E-538.79459848
CTTATAC4600.038.426271
Produced by FastQC (version 0.11.5)