FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398457
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40960.29289423986579494No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGTCGAGAGTGCTGGAGCTTGTATATGAG39600.283169235807751No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37180.2658644491750551No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36050.25778411492094505No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35520.25399422363361907No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC33760.24140892426438568No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG32860.23497325981420952No Hit
GATTATTACTGCAGCTCATATACAAGCTCCAGCACTCTCGACGTATTCGG30890.2208863054066017No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT30090.2151657147842229No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC29160.20851552818570754No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT27730.1982899724482054No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26130.1868487912034478No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA25780.18434603280615705No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG25340.18119970796384874No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24830.17755283144208225No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTTCCAACAGCACCCAGGCAAAGC24590.1758366542553686No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGTCGAGAGTGCTG23940.1711886743746858No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG23780.17004455625021006No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTTCCAACAGCA23780.17004455625021006No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC23610.16882893074295455No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23040.16475300992450967No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22980.16432396562783125No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGCGAGG22070.15781679379487534No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22030.15753076426375642No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21460.15345484344531152No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21410.15309730653141285No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG21100.15088057766524104No Hit
GTGCTGGAGCTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC21060.1505945481341221No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21040.15045153336856262No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20400.1458750608706596No Hit
CTCCAGCACTCTCGACGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT20190.14437340583228514No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20080.14358682462170808No Hit
GCTTATAACTATGTCTCCTGGTTCCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAGTAAT19790.14151311052109575No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19620.14029748501384026No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT19450.13908185950658475No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19100.13657910110929403No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18030.1289278111518624No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17430.12463736818507827No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17320.12385078697450119No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17060.12199159502222806No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16740.11970335877327656No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16640.11898828494547921No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16270.116342511782629No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16070.1149123641270343No Hit
GTGTATTACTGTGCGGCGAGGGTAGTTCGGAAAGACCAGCACTGGGGCCA16030.11462633459591534No Hit
GCCCAGAGATGGTCAGCGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG15940.11398276815089775No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15770.11276714264364224No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC15300.10940629565299469No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15120.10811916276295946No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14910.10661750772458503No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14900.10654600034180528No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCTCCAGCACTCTCGA14840.10611695604512687No Hit
GCCTGGAGCCCAGAGATGGTCAGCGAGGCCGTGTTGCCAGACTTGGAGCC14640.10468680838953218No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGTCGAGAGTGCTGG14590.10432927147563349No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACGTCGAGAGTG14400.10297063120281855No Hit
GGTTCCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTC14230.10175500569556305No Hit
CCCCTGAGGGCCGATTACTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGAACCAGGAGA14090.10075390233664674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAC150.002047765571.4803260
GGTATCA13550.041.8652421
ACCGGAG750.001463758238.76960870
GCAATAC451.10195455E-437.7171333
TCTGGAG12500.037.21882670
GTATCAA32500.036.372431
GTGGTAT3950.032.6785621
TATCAAC36750.032.3382262
ATCAACG38300.031.109253
TGGTATC4050.031.0207422
TCAACGC39050.030.4193924
CAACGCA39800.029.7608875
AACGCAG40250.029.5124786
CGCAGAG42400.028.096028
CAGAGTA43400.027.36848610
AGAGTAC43450.027.17883711
GCAGAGT44300.026.8909979
TAATCGT1409.837055E-425.96179270
CTCGCTA801.07026965E-425.44996312
CGGCTCT11450.025.21498921
Produced by FastQC (version 0.11.5)