FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046793
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCGTCTCGCCCAGAGACACAGCCAGGGAGGCTGGAGA101640.9709656063806311No Hit
CTATTATACAGCTCCGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA73880.7057746851574285No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71630.68428046423696No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC63920.6106269338828211No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48120.45968973808575336No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA48040.4589254991196922No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG45860.43809998729452715No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCA45840.43790892755301186No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44730.4273051118989141No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG44500.4251079248714884No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44250.4227196781025475No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43080.4115426832239038No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT42650.40743489878132544No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41120.39281882855540684No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC37040.3538426412862906No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35820.3421879970538588No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35390.33808021261128035No Hit
TCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCTCAGGGACC31690.30273416043095436No Hit
AGCCAGGACAGTCTCCTTCACTTCTCATTTTCTGGGCATCTTCCCGGGGA31690.30273416043095436No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC30580.2921303447768565No Hit
GAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCTCAGGGACCCCG30120.2877359707220052No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27740.26499986148168736No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26500.25315415750774034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26440.2525809782831945No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26060.24895084319440422No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24790.23681854960818424No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23960.2288895703353003No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT23880.22812533136923918No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22650.21637515726604972No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22620.21608856765377682No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT22240.21245843256498656No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22190.2119807832111984No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22190.2119807832111984No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21960.20978359618377274No Hit
GTGAAGGAGACTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGTATAATAGACT21810.20835064812240817No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT21120.20175908704013115No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20960.20023060910800894No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20940.20003954936649365No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT20870.19937084027119018No Hit
GTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCTC20780.19851107143437144No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20700.19774683246831035No Hit
GTCTCCAGCCTCCCTGGCTGTGTCTCTGGGCGAGACGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTATTATA20440.19526305582861178No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20320.19411669737952011No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA20190.1928748090596708No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC19760.18876702461709238No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19510.18637877784815146No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG19450.18580559862360563No Hit
GAAGGAGACTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGTATAATAGACTCTGG19100.1824620531470883No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA19010.18160228431026956No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18930.18083804534420844No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18590.17759002973844876No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18230.17415095439117378No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18090.17281353620056686No Hit
CCACTGAATCGCTCAGGGACCCCGGATCCCCGGGAAGATGCCCAGAAAATGAGAAGTGAAGGAGACTGTCCTGG18020.1721448271052634No Hit
ATTTTGGCCAGGGCACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA17980.17176270762223286No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG17790.16994764007783775No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17390.16612644524753223No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG17120.163547138737076No Hit
TTATTACTCCATTCAATTTTGGCCAGGGCACCAAGCTGGAGATCAAACGA16690.15943935429449757No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16670.15924829455298228No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA16270.1554270997226768No Hit
GCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCC16190.1546628607566157No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTGCCCTGGCCAAAATTG16160.15437627114434277No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16080.15361203217828168No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGGTGACCCAGTCTCCAGCCTCCCTGGCTGTGTCTCTGGGCGAGACGG15940.15227461398767472No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT15890.15179696463388656No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15740.150364016572522No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15380.14692494122524702No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC15220.1453964632931248No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGGTGA14950.1428171567826686No Hit
GGGCACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA14900.1423395074288804No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14810.14147973859206167No Hit
CAATTCACTCTCACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAACAATATTTTATTACT14560.1390914918231207No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT14510.13861384246933253No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG14490.13842278272781725No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14430.1378496035032714No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC14160.1352702969928152No Hit
GGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCT14060.1343149982852388No Hit
GCTAAGTAGTTCTTATTGTCGGAGCTGTATAATAGACTCTGGCTGGACTT13980.13355075931917773No Hit
CACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAAC13960.13335969957766244No Hit
GCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCTCAGGGACCCCGGAT13950.1332641697069048No Hit
CTCAGGGACCCCGGATCCCCGGGAAGATGCCCAGAAAATGAGAAGTGAAGGAGACTGTCCTGGCTTCTGCTGGTA13800.13183122164554023No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13300.12705472810765833No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG13150.12562178004629376No Hit
ATTCAATTTTGGCCAGGGCACCAAGCTGGAGATCAAACGAACTGTGGCTG13080.12495307095099031No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12970.12390224237265629No Hit
CTCCTTCACTTCTCATTTTCTGGGCATCTTCCCGGGGATCCGGGGTCCCT12920.1234245930188681No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGTATAATAGAC12880.12304247353583754No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12770.12199164495750353No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC12760.12189611508674589No Hit
GGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAAT12710.12141846573295771No Hit
GTCTCCTTCACTTCTCATTTTCTGGGCATCTTCCCGGGGATCCGGGGTCCCTGAGCGATTCAGTGGCTCCGGGT12680.12113187612068481No Hit
GTCCCAGACCCGGAGCCACTGAATCGCTCAGGGACCCCGGATCCCCGGGA12510.11950786831780495No Hit
CCCTGGCTGTGTCTCTGGGCGAGACGGCCACCATCAACTGCAAGTCCAGC12500.11941233844704731No Hit
GTCTCTGGGCGAGACGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTATTATACAGCTCCGACAATAAGAACTA12470.1191257488347744No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG12430.11874362935174385No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT12330.11778833064416748No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA12300.11750174103189455No Hit
ATTCAGTGGCTCCGGGTCTGGGACACAATTCACTCTCACCATCACCAGCC12250.11702409167810637No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCT12000.11463584490916541No Hit
CCCAGAGACACAGCCAGGGAGGCTGGAGACTGGGTCACCACGATGTCCCC11900.11368054620158904No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11780.11253418775249739No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG11770.11243865788173975No Hit
GGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGC11670.11148335917416337No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA11520.11005041111279881No Hit
GTTTATTACTGTCAACAATATTTTATTACTCCATTCAATTTTGGCCAGGGCACCAAGCTGGAGATCAAACGAA11510.10995488124204117No Hit
CCCCGGATCCCCGGGAAGATGCCCAGAAAATGAGAAGTGAAGGAGACTGT11460.10947723188825297No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG11370.10861746305143424No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC11350.10842640330991896No Hit
GAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11270.10766216434385785No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11210.10708898511931203No Hit
AAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT11170.10670686563628148No Hit
CAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCA11170.10670686563628148No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11160.10661133576552384No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG11140.10642027602400857No Hit
GAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTG11010.10517838770415927No Hit
CATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATG10970.10479626822112872No Hit
GACCCGGAGCCACTGAATCGCTCAGGGACCCCGGATCCCCGGGAAGATGCCCAGAAAATGAGAAGTGAAGGAGA10870.10384096951355234No Hit
GGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGA10840.10355437990127944No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10830.1034588500305218No Hit
GAATCGCTCAGGGACCCCGGATCCCCGGGAAGATGCCCAGAAAATGAGAAGTGAAGGAGACTGTCCTGGCTTCTG10750.10269461106446069No Hit
GATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGG10730.10250355132294542No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10650.10173931235688433No Hit
GAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTA10480.10011530455400447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)