FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046793
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGACTCTGGCTGGACTTGCAGTTGATGGTGGCCGTCTCGCCCAGAGACACAGCCAGGGAGGCTGGAGA98050.9366703827786392No Hit
CTATTATACAGCTCCGACAATAAGAACTACTTAGCTTGGTACCAGCAGAA70500.6734855888413468No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC63330.6049906715081205No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC57810.5522581828499044No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC47110.4500412211392319No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47080.449754631526959No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA45610.43571174052558626No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT45370.433419023627403No Hit
GTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCA45110.43093524698770436No Hit
GTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTG44240.42262414823178984No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG42830.4091544364549629No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42390.4049511221416269No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40930.39100376101101175No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC38790.3705603686688772No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37710.3602431426270523No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37370.35699512702129266No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT35450.3386533918358262No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34150.3262345086373333No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC32420.3097078409962619No Hit
GAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCTCAGGGACCCCG32050.3061732357782293No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31070.2968113084439808No Hit
TCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCTCAGGGACC28360.2709227134686609No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG27850.2660506900600214No Hit
AGCCAGGACAGTCTCCTTCACTTCTCATTTTCTGGGCATCTTCCCGGGGA27550.26318479393729227No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27270.2605099575560784No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA26750.25554240427668123No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25880.24723130552076678No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23980.2290806300768156No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23760.22697897292014751No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23010.2198142326133247No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT22800.2178081053274143No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC22180.21188525334044075No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA21710.20739534941483176No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG21650.20682217019028595No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21630.20663111044877067No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20830.19898872078815966No Hit
GTCTCCAGCCTCCCTGGCTGTGTCTCTGGGCGAGACGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTATTATA20750.19822448182209856No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT20530.19612282466543052No Hit
GTGAAGGAGACTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGTATAATAGACT20460.19545411557012707No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20280.19373457789648957No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19840.1895312635831535No Hit
GTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCTC19640.18762066616800074No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG19100.1824620531470883No Hit
ATTTTGGCCAGGGCACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA18470.17644367128935712No Hit
GAAGGAGACTGTCCTGGCTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGTATAATAGACTCTGG18270.17453307387420436No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18230.17415095439117378No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT17990.1718582374929905No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT17940.17138058813920232No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17630.16841916214571553No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17510.16727280369662387No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17330.1655532660229864No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17310.16536220628147114No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG17110.16345160886631838No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16990.1623052504172267No Hit
TTATTACTCCATTCAATTTTGGCCAGGGCACCAAGCTGGAGATCAAACGA16850.16096783222661978No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16840.16087230235586214No Hit
CCACTGAATCGCTCAGGGACCCCGGATCCCCGGGAAGATGCCCAGAAAATGAGAAGTGAAGGAGACTGTCCTGG16780.1602991231313163No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16760.16010806338980105No Hit
GGATCTCTGGTGCCTGCGGGGACATCGTGGTGACCCAGTCTCCAGCCTCCCTGGCTGTGTCTCTGGGCGAGACGG16590.1584840555869212No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16080.15361203217828168No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTGCCCTGGCCAAAATTG16040.15322991269525113No Hit
GGGCACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCA15980.15265673347070527No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA15670.14969530747721851No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT15350.14663835161297412No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA15260.14577858277615535No Hit
AGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGGTGA15180.14501434381009426No Hit
GCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCC15060.1438679853610026No Hit
GCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCTCAGGGACCCCGGAT14830.14167079833357693No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT14700.14042891001372765No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14460.13813619311554434No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14430.1378496035032714No Hit
CAATTCACTCTCACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAACAATATTTTATTACT14370.13727642427872558No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAG14300.13660771518342213No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT14290.13651218531266449No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14190.1355568866050881No Hit
GGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAATCGCT14170.13536582686357285No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG14090.13460158789751175No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13990.13364628918993535No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13880.13259546061160132No Hit
GGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCACTGAAT13870.13249993074084368No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA13530.129251915135084No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG13500.1289653255228111No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC13240.12648154888311253No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGG13210.1261949592708396No Hit
ATTCAATTTTGGCCAGGGCACCAAGCTGGAGATCAAACGAACTGTGGCTG13140.12552625017553615No Hit
CACCATCACCAGCCTGCAGGCTGAGGATGTGGCAGTTTATTACTGTCAAC13100.1251441306925056No Hit
GTCTCCTTCACTTCTCATTTTCTGGGCATCTTCCCGGGGATCCGGGGTCCCTGAGCGATTCAGTGGCTCCGGGT12870.12294694366507992No Hit
CTCCTTCACTTCTCATTTTCTGGGCATCTTCCCGGGGATCCGGGGTCCCT12870.12294694366507992No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGTATAATAGAC12780.12208717482826117No Hit
CTCAGGGACCCCGGATCCCCGGGAAGATGCCCAGAAAATGAGAAGTGAAGGAGACTGTCCTGGCTTCTGCTGGTA12640.12074975663765425No Hit
GTCTCTGGGCGAGACGGCCACCATCAACTGCAAGTCCAGCCAGAGTCTATTATACAGCTCCGACAATAAGAACTA12510.11950786831780495No Hit
GTTTATTACTGTCAACAATATTTTATTACTCCATTCAATTTTGGCCAGGGCACCAAGCTGGAGATCAAACGAA12470.1191257488347744No Hit
ATTCAGTGGCTCCGGGTCTGGGACACAATTCACTCTCACCATCACCAGCC12360.11807492025644038No Hit
CCCTGGCTGTGTCTCTGGGCGAGACGGCCACCATCAACTGCAAGTCCAGC12280.11731068129037928No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC12240.11692856180734873No Hit
GCTAAGTAGTTCTTATTGTCGGAGCTGTATAATAGACTCTGGCTGGACTT12070.11530455400446887No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC12020.1148269046506807No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12010.11473137477992305No Hit
GTCCCAGACCCGGAGCCACTGAATCGCTCAGGGACCCCGGATCCCCGGGA11810.1128207773647703No Hit
CAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGA11770.11243865788173975No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11630.11110123969113282No Hit
GGCTGAGGATGTGGCAGTTTATTACTGTCAACAATATTTTATTACTCCATTCAATTTTGGCCAGGGCACCAAG11580.11062359033734463No Hit
GAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTA11570.110528060466587No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG11430.10919064227598006No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11240.10737557473158495No Hit
CCCCGGATCCCCGGGAAGATGCCCAGAAAATGAGAAGTGAAGGAGACTGT11040.10546497731643217No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10990.10498732796264401No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10980.10489179809188637No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT10970.10479626822112872No Hit
GAATCGCTCAGGGACCCCGGATCCCCGGGAAGATGCCCAGAAAATGAGAAGTGAAGGAGACTGTCCTGGCTTCTG10900.10412755912582526No Hit
GAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTG10870.10384096951355234No Hit
CAGTAATAAACTGCCACATCCTCAGCCTGCAGGCTGGTGATGGTGAGAGTGAATTGTGTCCCAGACCCGGAGCCA10640.10164378248612668No Hit
GGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTGCGGGGACATCGTGGTGACCCAGTCTCCAGCCTCC10610.10135719287385377No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10560.10087954352006558No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAG10470.10001977468324683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)