FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141476
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGG73920.6475826035764221No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGG68870.6033416383699701No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT54160.47447340110523567No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC46150.4043010978767841No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44140.38669231766589923No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC41200.36093619138729155No Hit
GTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCC40380.3537525099082241No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG40330.35331448054974435No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC38680.3388595117199135No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC37360.3272955366560488No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC34610.3032039219396641No Hit
GTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT31900.2794627307100631No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT31800.27858667199310366No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30490.26711030280093495No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29500.25843732150303644No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29230.2560719629672459No Hit
CTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC28140.24652292295238798No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCAC26490.23206795412255712No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGG26340.23075386604711795No Hit
GCCTTGGGCTGACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGACTGTCATCCCATG25620.22444624328500995No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC25480.2232197610812667No Hit
GGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCCAAGGCCAACCC24780.21708735006255056No Hit
GTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA24210.21209381537588173No Hit
GACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGA24090.2110425449155304No Hit
CTCTTGTTCTGGAAGCAATTCCAACATCGGAAGTAATTTTGTATCCTGGTACCAGCACCTCCCAGGAACGGCCCC23660.2072754924326048No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23630.20701267481751695No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG23050.20193153425915217No Hit
TTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCA22820.19991659921014546No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22640.19833969351961844No Hit
CCCCTGAGGGCCGCTGATCATTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATA22350.19579912324043605No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG21810.19106840616885507No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21710.19019234745189562No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGAGGGTGACGTTGGTC21640.189579106350024No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21300.1866005067123619No Hit
CCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG21170.18546163038031463No Hit
GTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCC21100.18484838927844302No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT20700.18134415441060522No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC19960.1748613199051053No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG19440.1703058145769162No Hit
ACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGAC19250.16864130301469327No Hit
CTTTAGCACTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGG18190.15935508061492312No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG18010.15777817492439614No Hit
CTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCC17830.15620126923386912No Hit
GGCCATCAGTGGCCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG17480.15313506372451108No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17280.15138294629059218No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA17210.15076970518872057No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC17010.14901758775480167No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT16960.14857955839632198No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG16830.1474406820642747No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16620.14560095875865983No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC16600.14542574701526795No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16580.14525053527187606No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16400.14367362958134905No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT16240.14227193563421395No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG15960.1398189712267275No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15730.13780403617772077No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCT15710.1376288244343289No Hit
ATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC15380.13473783066836273No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14820.12983190185338983No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG14750.12921866075151822No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14680.1286054196496466No Hit
GTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACC14620.12807978441947093No Hit
CTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG14500.1270285139591196No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTC14490.12694090808742364No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCG14340.12562682001198447No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14290.12518879065350477No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC14190.12431273193654532No Hit
CCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAG13970.12238540275923454No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGAC13920.12194737340075482No Hit
GACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAATTCCAACAT13910.12185976752905887No Hit
GTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGAATTGCTTCCAG13830.12115892055549131No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13810.12098370881209942No Hit
GTACCAGCACCTCCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGA13810.12098370881209942No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG13510.11835553266122108No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG13490.1181803209178292No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13480.11809271504613324No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTT13100.11476369192168737No Hit
GTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG12930.1132743921028563No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAG12890.11292396861607253No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTC12880.11283636274437658No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12580.11020818659349824No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12580.11020818659349824No Hit
GAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12570.1101205807218023No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGAC12530.10977015723501853No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG12520.10968255136332258No Hit
TCCTCGGACCGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACC12360.10828085741618747No Hit
TTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTG12150.10644113411057263No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA12130.10626592236718073No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA12120.10617831649548479No Hit
GCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGT11990.10503944016343751No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11990.10503944016343751No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAG11950.10468901667665374No Hit
ACGTCACCCTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAG11910.10433859318986996No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGTCCGAGGATG11890.10416338144647808No Hit
GTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCT11850.1038129579596943No Hit
GACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGG11650.1020608405257754No Hit
GTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAATTCCAACATCGGAAGTAATTTTGTATC11640.10197323465407945No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11530.10100957006542408No Hit
GCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGT11520.1009219641937281No Hit
GTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGC11510.10083435832203218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002371556168.8821539
ACGGTAC200.006870763452.65622352
AGGGGGG3400.052.44436670
ACTCGAC3650.046.69711327
TCGACGT3600.046.4436129
GGTATCA11400.045.9712221
CTCGACG3700.045.17250428
ACGAGAC3850.044.19758222
CGACGTC3750.043.68362830
GACTCGA4000.042.59242626
TCTCCGA4100.042.32338711
AGACTCG4000.041.71861625
GTATCAA32150.041.7047421
CCCACGA4200.041.31568519
CGTATGC3050.040.7692641
CTTATAC4350.040.6803171
CGAGACT4200.040.51977523
CACGAGA4200.040.5073521
TCTCGTA2300.040.38605538
TCGTATG2650.040.35397340
Produced by FastQC (version 0.11.5)