FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141476
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGG65340.5724167656613017No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGGCCAGGGAGG62260.5454341571789508No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT48180.4220850898310608No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC46560.4078929386163178No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA41210.3610237972589875No Hit
GTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCTGGGACCC40230.3524384218327849No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38460.3369321825426027No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37510.328609624731488No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34800.304868433501887No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33110.29006304118527243No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG31600.2768345545591848No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31110.27254186684608345No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31090.2723666551026916No Hit
GTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCACCCTCAGCGTCT28790.2522173046126243No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28350.2483626462580028No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28280.2477494051561312No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27150.23784994165448944No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGG25270.2213800377756519No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG24430.21402114455319254No Hit
GCCTTGGGCTGACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGACTGTCATCCCATG24180.21183099776079392No Hit
TTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCA24120.21130536253061824No Hit
CTCTTGTTCTGGAAGCAATTCCAACATCGGAAGTAATTTTGTATCCTGGTACCAGCACCTCCCAGGAACGGCCCC24070.21086733317213854No Hit
GGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCCAAGGCCAACCC23980.21007888032687502No Hit
GTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA23240.20359604582137514No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC22950.20105547554219277No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22760.1993909639799698No Hit
CTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22560.1976388465460509No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCAC22310.1954486997536523No Hit
GACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGA22000.192732917731078No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21870.19159404139903072No Hit
CCCCTGAGGGCCGCTGATCATTCTTATAGATGAGAAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATA21700.19010474158019966No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21630.18949150047832805No Hit
GTGCTAAAGTCTTCGGACCTGGGACCAACGTCACCCTCCTAGGTCAGCCC21390.1873889595576254No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGAGGGTGACGTTGGTC20570.18020527807855793No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGG19920.17451089641832154No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19150.16776524429773382No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCGGT19090.16723960906755814No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18850.16513706814685547No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC18190.15935508061492312No Hit
CCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG18010.15777817492439614No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17760.15558802813199754No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17460.15295985198111917No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17390.15234661087924756No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17280.15138294629059218No Hit
CTTTAGCACTCAGACTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGGCCACTGATGG17220.15085731106041653No Hit
GGCCATCAGTGGCCTCCGGTCCGAGGATGAGGCTGATTATTACTGTGCAG17060.1494556171132814No Hit
CTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCC16990.1488423760114098No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC16920.14822913490953818No Hit
GTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGAATTGCTTCCAG16450.14411165893982877No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16170.14165869453234234No Hit
ACCTAGGAGGGTGACGTTGGTCCCAGGTCCGAAGACTTTAGCACTCAGAC16100.14104545343047073No Hit
GGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG15880.13911812425315995No Hit
GTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTT15540.13613952461549783No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15500.13578910112871403No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15430.13517586002684243No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15420.1350882541551465No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15040.1317592310307006No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14750.12921866075151822No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG14550.12746654331759932No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14510.12711611983081553No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG14430.126415272857248No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC14330.12553921414028854No Hit
GAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAG14250.12483836716672098No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG14220.12457554955163314No Hit
GTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGACC14170.12413752019315343No Hit
CTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAG14090.12343667321958586No Hit
GTCCGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGTCTGAGTGCTAAAGTCTTCGGACCTGGGAC13940.1221225851441467No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCT13910.12185976752905887No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACCTGGAGCCAGAGAATCGGTC13760.1205456794536197No Hit
GGAGTGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGC13240.11599017412543058No Hit
CCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT13170.11537693302355898No Hit
GTGCCAGACCTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG12950.11344960384624819No Hit
ATTCAGGACATTCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC12820.11231072751420092No Hit
GTACCAGCACCTCCCAGGAACGGCCCCCAAACTTCTCATCTATAAGAATGATCAGCGGCCCTCAGGGGTCCCTGA12580.11020818659349824No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA12560.11003297485010635No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCG12020.10530225777852535No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12000.10512704603513345No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG11920.1044261990615659No Hit
GTTCCTGGGAGGTGCTGGTACCAGGATACAAAATTACTTCCGATGTTGGA11920.1044261990615659No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAG11870.10398816970308618No Hit
GACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGCGGGTCACCATCTCTTGTTCTGGAAGCAATTCCAACAT11840.10372535208799834No Hit
CTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTG11820.10355014034460647No Hit
GCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA11530.10100957006542408No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC11520.1009219641937281No Hit
GTCCCTGACCGATTCTCTGGCTCCAGGTCTGGCACCTCAGCCTCCCTGGC11510.10083435832203218No Hit
GAACAAGAGATGGTGACCCGCTGCCCGGGGGTCCCAGACGCTGAGGGTGG11470.1004839348352484No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11470.1004839348352484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.043.909281
TGGTATC3000.043.0759352
CTAACGC405.4563017E-542.494253
GTGGTAT3250.041.8826941
GTATCAA34200.041.7908441
TATCAAC39550.036.027982
ATCAACG41650.034.2810753
TCAACGC41900.033.912784
CAACGCA42650.033.3149645
AACGCAG43900.032.443796
CGGTACC652.2155318E-532.06447645
TTAACGG652.4366307E-531.62141435
GCCAGAG27000.031.06035670
CGCAGAG46150.031.0093388
ACGCAGA46000.030.962667
AGAGTAC47400.030.2619711
GCAGAGT47650.030.033189
TAACGGC802.8952982E-630.00493436
CAGAGTA47800.029.9376210
CCTAACG605.898195E-428.339432
Produced by FastQC (version 0.11.5)