FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763666
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAGAGCTGCCCTGACACTGTCAGGTGTGCAGC34870.45661323143887506No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA33520.43893534608061635No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT32870.4304237716488622No Hit
GACCTAGGACGATGACCTTGGTCCCATTTCCGAAGACAAAGGCACCACCATAATAGATCAGGCAATAATAGTCAG30350.397425052313446No Hit
CCATAATAGATCAGGCAATAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTCTGCCC27280.3572242315357761No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCAGCCCAGGGTCCA26970.35316486526832414No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26550.34766507871242136No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG26520.34727223681557123No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG25570.3348322434153151No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC25060.32815393116886177No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24870.32566593248881054No Hit
GTCCAGGAGTGTTTGTTGCTTATACTATAAATCAGGGCCCTGGGTGCCTG24860.32553498518986046No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCA24760.32422551220035983No Hit
GTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCAC23550.308380889027402No Hit
GATTTATAGTATAAGCAACAAACACTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAGAGCTGC22640.29646468482294613No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22600.2959408956271459No Hit
CTATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACCAAGGTCATCGTCCTAGGTCAGCCCAAGGCCAACCCCA21890.28664363740169135No Hit
GCCTTGGGCTGACCTAGGACGATGACCTTGGTCCCATTTCCGAAGACAAA20800.2723703818161343No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20280.2655611222707309No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTATTG19170.2510259720872738No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC19010.2489308153040727No Hit
CACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC17670.23138387724476409No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG16990.2224794609161597No Hit
ACCTAGGACGATGACCTTGGTCCCATTTCCGAAGACAAAGGCACCACCATAATAGATCAGGCAATAATAGTCAGC16980.22234851361720961No Hit
CTATAAATCAGGGCCCTGGGTGCCTGTCCAGGTTTCTGCTGGAACCAGTTTGGATGGTAGTCACTGGTGACTGTT16320.21370599188650535No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16050.21017041481485363No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16050.21017041481485363No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGATGACCTTGGTCCCATTTCCGAAGACAAAGGCACCAC15840.20742052153690224No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15790.20676578504215193No Hit
GTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCTCTGGA15660.2050634701558011No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15610.20440873366105078No Hit
GCCTGAGGACGAGGCTGACTATTATTGCCTGATCTATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACCAAGGT15130.1981232633114477No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14660.19196874026079463No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTCTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTC13230.1732432765109354No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG13130.17193380352143475No Hit
GTGTCAGGGCAGCTCTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTCCAGGAGTGTTTGTTGCTTATA12630.16538643857393154No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12600.16499359667708136No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCAGCCCAGGGTCCAATTCTCAGAC12380.16211275610017992No Hit
ATTATTGCCTGATCTATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACC12260.16054138851277916No Hit
ATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACCAAGGTCATCGTCCTAGGTCAGCCCAAGGCCAACCCCA12130.15883907362642832No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12130.15883907362642832No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG12130.15883907362642832No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG11970.15674391684322728No Hit
GACTATTATTGCCTGATCTATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACCAAGGTCATCGTCCTAGGTCAG11670.15281549787472534No Hit
GAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTATTGCCTGATCTATTATGGTGGTG11660.15268455057577526No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCAC11630.15229170867892508No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11430.1496727626999238No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA11360.14875613160727333No Hit
GGGCAGCTCTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTCCAG10760.14089929367026946No Hit
CCTGACAGTGTCAGGGCAGCTCTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTCCAGGAGTGTTTGTTG10710.14024455717551915No Hit
GTAATAGACGGCCGTGTCCTCAAATGTCAGGCTGTTCAGTACCATGTAGGCTGTGCTGATGGAGGTGTTCCTGGT10700.14011360987656907No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10530.13788750579441797No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10350.13553045441331682No Hit
GTCTATTACTGTGCGAGAGGCCGGTCCGCTTACGATTTTTGGAGTGGTTATTCTGGGGACTGGTACTTCGATCT10220.13382813952696598No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10080.13199487734166507No Hit
GGCAATAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTCTGCCCCCAAGGAGGGAGC10050.13160203544481489No Hit
CCTTGGGGGCAGAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACG9880.1293759313626638No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9710.1271498272805127No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9570.12531656509521177No Hit
ATAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG9550.12505467049731164No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9520.12466182860046146No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG9480.1241380394046612No Hit
GCTCTGGAACAGTCACCAGTGACTACCATCCAAACTGGTTCCAGCAGAAA9470.12400709210571115No Hit
GTACTGAACAGCCTGACATTTGAGGACACGGCCGTCTATTACTGTGCGAG9320.12204288262146017No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG9280.1215190934256599No Hit
CTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCTCTGGAACAGTCACCAGTGACTA9240.12099530422985964No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8980.11759067445715797No Hit
CCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCTCTGGAACAGTCACCAGTGA8920.11680499066345758No Hit
AACCTGGACAGGCACCCAGGGCCCTGATTTATAGTATAAGCAACAAACAC8820.11549551767395694No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTC8790.11510267577710676No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8540.11182899330335515No Hit
GTTCAGTACCATGTAGGCTGTGCTGATGGAGGTGTTCCTGGTCACGGTGA8520.111567098705455No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC8470.11091236221070468No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG8440.11051952031385448No Hit
GGCACCCAGGGCCCTGATTTATAGTATAAGCAACAAACACTCCTGGACCC8400.10999573111805422No Hit
CTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTATTGCCTGATCTA8370.10960288922120404No Hit
CACCTGTGCTTCCAGCTCTGGAACAGTCACCAGTGACTACCATCCAAACT8340.10921004732435385No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8330.10907910002540377No Hit
CTCTCACCTGTGCTTCCAGCTCTGGAACAGTCACCAGTGACTACCATCCA8320.10894815272645371No Hit
GAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCTCTG8310.10881720542750364No Hit
TCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCAC8280.10842436353065345No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG8090.10593636485060223No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8070.1056744702527021No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8010.10488878645900171No Hit
CTCTGGAACAGTCACCAGTGACTACCATCCAAACTGGTTCCAGCAGAAAC7990.10462689186110158No Hit
CCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCTCTGGAACAGTC7960.10423404996425138No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG7890.10331741887160094No Hit
GTCCAGGTTTCTGCTGGAACCAGTTTGGATGGTAGTCACTGGTGACTGTT7860.10292457697475076No Hit
GATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGG7840.10266268237685063No Hit
TTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCA7840.10266268237685063No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7780.10187699858315022No Hit
GGCCTGGAAGGCAGATGGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA7780.10187699858315022No Hit
CTTCCAATATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTC7730.1012222620883999No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTACG150.002210260470.1141650
CGCTATT200.0078317150.93984616
AATGGTA5200.047.67514470
GGTATCA5150.040.2348821
GTATCAA14250.039.5706631
TCGTATG702.1902451E-839.4230640
TATACAC1050.038.8214763
TAGCGCT901.4188117E-1037.8198929
CTAGCGC901.4370016E-1037.79508228
ACTAGCG952.564775E-1035.7870927
GACTAGC952.582965E-1035.77771426
CACGAGA1052.0008883E-1135.57936521
CGTATGC807.0025635E-834.54802341
TATCAAC16250.034.491392
TCTCGTA807.3461706E-834.3603538
CATACAC400.002800983633.9687923
GTATAAT400.002800983633.9687921
CCCACGA1103.45608E-1133.95989619
ATCAACG17150.032.6813473
TCAACGC17150.032.483284
Produced by FastQC (version 0.11.5)