FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences763666
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAGAGCTGCCCTGACACTGTCAGGTGTGCAGC34190.4477088151102707No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA30530.39978210369454714No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT28880.37817579936778645No Hit
GACCTAGGACGATGACCTTGGTCCCATTTCCGAAGACAAAGGCACCACCATAATAGATCAGGCAATAATAGTCAG28690.3756878006877352No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC28000.36665243706018075No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCAGCCCAGGGTCCA27940.3658667532664804No Hit
CCATAATAGATCAGGCAATAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTCTGCCC25830.3382368731880167No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG24680.3231779338087593No Hit
GTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCAC24260.31767814725285665No Hit
GTCCAGGAGTGTTTGTTGCTTATACTATAAATCAGGGCCCTGGGTGCCTG24220.3171543580570564No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23740.31086888770745325No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC23740.31086888770745325No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCA23570.3086427836253022No Hit
GATTTATAGTATAAGCAACAAACACTCCTGGACCCCTGCCCGATTCTCAGGCTCCCTCCTTGGGGGCAGAGCTGC23440.3069404687389513No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG22260.29148868746284373No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC22090.2892625833806926No Hit
CTATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACCAAGGTCATCGTCCTAGGTCAGCCCAAGGCCAACCCCA20750.2717156453213839No Hit
GCCTTGGGCTGACCTAGGACGATGACCTTGGTCCCATTTCCGAAGACAAA19780.2590137573232277No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17870.23400282322376537No Hit
GCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTATTG17660.231252929945814No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG16960.22208661901930948No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC16750.21933672574135812No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16750.21933672574135812No Hit
CACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGC16000.20951567832010332No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGATGACCTTGGTCCCATTTCCGAAGACAAAGGCACCAC15990.2093847310211532No Hit
CTATAAATCAGGGCCCTGGGTGCCTGTCCAGGTTTCTGCTGGAACCAGTTTGGATGGTAGTCACTGGTGACTGTT15950.20886094182535297No Hit
GTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCTCTGGA15880.2079443107327025No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG15860.2076824161348024No Hit
GCCTGAGGACGAGGCTGACTATTATTGCCTGATCTATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACCAAGGT15680.20532536475370125No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC14740.19301631865239516No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14390.18843316318914288No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT14100.18463569151959103No Hit
ACCTAGGACGATGACCTTGGTCCCATTTCCGAAGACAAAGGCACCACCATAATAGATCAGGCAATAATAGTCAGC14080.1843737969216909No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG13520.1770407481804873No Hit
GTGTCAGGGCAGCTCTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTCCAGGAGTGTTTGTTGCTTATA13140.17206475082038483No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG12950.1695767521403336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12440.1628984398938803No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA12410.1625055979970301No Hit
GAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTATTGCCTGATCTATTATGGTGGTG12100.1584462317295781No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11840.15504160195687644No Hit
GTCCAATTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCAC11770.154124970864226No Hit
TCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTCTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTC11610.15202981408102495No Hit
ATTATTGCCTGATCTATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACC11450.14993465729782393No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11310.14810139511252302No Hit
CCTCTCTTTCTGTTCCTCCTCACTTGCAGCCCAGGGTCCAATTCTCAGAC11240.14718476401987257No Hit
GACTATTATTGCCTGATCTATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACCAAGGTCATCGTCCTAGGTCAG11090.1452205545356216No Hit
ATTATGGTGGTGCCTTTGTCTTCGGAAATGGGACCAAGGTCATCGTCCTAGGTCAGCCCAAGGCCAACCCCA11010.14417297614402108No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA10760.14089929367026946No Hit
GGGCAGCTCTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTCCAG10520.13775655849546792No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10440.1367089801038674No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT10240.1340900341248661No Hit
GTAATAGACGGCCGTGTCCTCAAATGTCAGGCTGTTCAGTACCATGTAGGCTGTGCTGATGGAGGTGTTCCTGGT10240.1340900341248661No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC10130.1326496138364154No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG10030.13134014084691475No Hit
GTCTATTACTGTGCGAGAGGCCGGTCCGCTTACGATTTTTGGAGTGGTTATTCTGGGGACTGGTACTTCGATCT9950.13029256245531423No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT9590.1255784596931119No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC9540.12492372319836159No Hit
CCTTGGGGGCAGAGCTGCCCTGACACTGTCAGGTGTGCAGCCTGAGGACG9430.12348330290991089No Hit
GGCAATAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAGCTCTGCCCCCAAGGAGGGAGC9280.1215190934256599No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT9270.12138814612670985No Hit
CTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCTCTGGAACAGTCACCAGTGACTA9140.119685831240359No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA9080.11890014744665861No Hit
GTACTGAACAGCCTGACATTTGAGGACACGGCCGTCTATTACTGTGCGAG9050.11850730554980843No Hit
ATAATAGTCAGCCTCGTCCTCAGGCTGCACACCTGACAGTGTCAGGGCAG9020.11811446365295823No Hit
CCTGACAGTGTCAGGGCAGCTCTGCCCCCAAGGAGGGAGCCTGAGAATCGGGCAGGGGTCCAGGAGTGTTTGTTG9010.11798351635400817No Hit
GCTCTGGAACAGTCACCAGTGACTACCATCCAAACTGGTTCCAGCAGAAA8950.11719783256030779No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG8910.11667404336450753No Hit
GAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCTCTG8750.1145788865813065No Hit
GTTCAGTACCATGTAGGCTGTGCTGATGGAGGTGTTCCTGGTCACGGTGA8590.11248372979810546No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC8490.11117425680860481No Hit
AACCTGGACAGGCACCCAGGGCCCTGATTTATAGTATAAGCAACAAACAC8450.11065046761280456No Hit
CTCTCACCTGTGCTTCCAGCTCTGGAACAGTCACCAGTGACTACCATCCA8440.11051952031385448No Hit
GTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTT8430.11038857301490443No Hit
CCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTCCAGCTCTGGAACAGTCACCAGTGA8360.10947194192225398No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC8290.10855531082960353No Hit
GGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGA8260.10816246893275332No Hit
TTCTCAGACTGTGGTGACTCAGGAGCCCTCACTGAGTGTGTCCCCAGGAGGGACAGTCACTCTCACCTGTGCTTC8220.10763867973695306No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG8190.10724583784010287No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC8180.10711489054115282No Hit
CTCTGGAACAGTCACCAGTGACTACCATCCAAACTGGTTCCAGCAGAAAC8090.10593636485060223No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG8000.10475783916005166No Hit
GGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGC7830.10253173507790055No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG7750.10148415668630004No Hit
CTGTCAGGTGTGCAGCCTGAGGACGAGGCTGACTATTATTGCCTGATCTA7740.10135320938734996No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC7740.10135320938734996No Hit
GGCACCCAGGGCCCTGATTTATAGTATAAGCAACAAACACTCCTGGACCC7710.10096036749049977No Hit
ATTCTCAGGCTCCCTCCTTGGGGGCAGAGCTGCCCTGACACTGTCAGGTG7670.10043657829469951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)