FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164656
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGA47670.409305408635683No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31110.26711750078993285No Hit
GTGTATTACTGTGCGAGAGATTGGGGATATTACACCCACTTAGACTGGGGCCAGGGAACCCTAGTCACCGTCTC29480.2531219518896567No Hit
GTCTAAGTGGGTGTAATATCCCCAATCTCTCGCACAGTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAG29160.2503743594675166No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT29000.2490005632564465No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTTCTGGTGGGGCTGGATCCGGCA28160.24178813314832875No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA26430.22693396161613386No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25510.21903463340248105No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24570.21096358066244456No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTATAGTCGTTGTCTGCAAATGAGCTGCAGTAATAAT23970.20581184487093188No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23900.20521080902858868No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC23780.20418046187028616No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23310.2001449355002679No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG22730.19516492423513895No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCGGTCACCATCTCCTG22290.19138698465469633No Hit
CACTTAGACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAGCATCCCC21850.1876090450742537No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21830.18743732054786993No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21710.18640697338956738No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT21390.1836593809674273No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21100.18116937533486283No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20350.17472970559547196No Hit
GGCCTGGAGTGGGTTGGCAGTATTTTTCACAGTGGGACAACCCACTCCAA20200.17344177164759378No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGAACTGATTCTTGGACGTGTCTA19930.1711234905414131No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC19870.1706083169622618No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC19460.16708796417139482No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19400.16657279059224356No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19330.1659717547499004No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTATAGTCGTTGT19320.16588589248670854No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC18690.1604765699056202No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18250.15669863032517758No Hit
GATTATTACTGCAGCTCATTTGCAGACAACGACTATAATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT18240.15661276806198568No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC18120.15558242090368313No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18110.15549655864049128No Hit
GTCCCTCACCTGCGCTGTCTCTGGTTACTCCATCAGCAGTGGTTTCTGGT17580.1509458586913217No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17260.14819826626918164No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16870.14484963800469838No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16850.14467791347831463No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG16420.14098583616106386No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTATAGTCGTTGTCTGCAAATGAGCTG16400.1408141116346801No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16240.13944031542361007No Hit
GTATTACTGTGCGAGAGATTGGGGATATTACACCCACTTAGACTGGGGCC16040.1377230701597725No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATTT15870.13626341168551057No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15630.1342027173689055No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15340.13171271173634105No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT15250.13093995136761413No Hit
ACCCACTCCAATCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAATCAGTTCTCCCTGAAG15070.12939443063016032No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC15060.12930856836696844No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG14800.1270761495239796No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14790.12699028726078773No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC14710.12630338915525272No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14550.12492959294418265No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCGGTCAC14490.12441441936503139No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT14310.12286889862757758No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14260.12243958731161819No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14180.12175268920608318No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC14150.12149510241650753No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG14140.12140924015331565No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13900.11934854583671058No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13900.11934854583671058No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13680.11745957604648927No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13380.11488370815073293No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACTAGGGTTCCCTGGCCCCAGTCTAAGTGGGTGTAATATC13250.1137674987292385No Hit
CTCCAGGCCCTTCCCTGGGGGCTGCCGGATCCAGCCCCACCAGAAACCAC13120.11265128930774408No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12830.11016128367517962No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG12750.1094743855696446No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGCTGAAGCC12600.10818645162176642No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12520.1074995535162314No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC12480.10715610446346388No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACTAGGGTTCCCTGG12070.10363575167259688No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12040.10337816488302125No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGCTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCG11820.10148919509279992No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA11800.10131747056641618No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG11760.10097402151364866No Hit
CTCCAATCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCA11710.10054471019768926No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)