FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164656
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGA45110.3873246692585622No Hit
GTGTATTACTGTGCGAGAGATTGGGGATATTACACCCACTTAGACTGGGGCCAGGGAACCCTAGTCACCGTCTC29230.25097539530985974No Hit
GTCTAAGTGGGTGTAATATCCCCAATCTCTCGCACAGTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAG28390.24376296520174198No Hit
GTCTCTGGTTACTCCATCAGCAGTGGTTTCTGGTGGGGCTGGATCCGGCA28110.24135882183236937No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC27160.23320190682914096No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT27140.2330301823027572No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24610.21130702971521204No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24420.20967564671456637No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA24010.20615529392369936No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23480.20160459397452982No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC22980.19731148081493594No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCGGTCACCATCTCCTG22980.19731148081493594No Hit
CTCCTGGACAGTCGGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT22490.19310422991853388No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTATAGTCGTTGTCTGCAAATGAGCTGCAGTAATAAT22430.1925890563393826No Hit
CACTTAGACTGGGGCCAGGGAACCCTAGTCACCGTCTCCTCAGCATCCCC21830.18743732054786993No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21770.18692214696871867No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC21730.18657869791595114No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21590.18537662623126486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21280.18271489607231664No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20480.1758459150169664No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC20250.1738710829635532No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG20010.17181038864694811No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19980.17155280185737248No Hit
GGCCTGGAGTGGGTTGGCAGTATTTTTCACAGTGGGACAACCCACTCCAA19720.16932038301438362No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19680.16897693396161614No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC19520.16760313775054608No Hit
CAGTAATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAGCTTCAGGGAGAACTGATTCTTGGACGTGTCTA19420.16674451511862728No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18690.1604765699056202No Hit
GTCCCTCACCTGCGCTGTCTCTGGTTACTCCATCAGCAGTGGTTTCTGGT18050.15498138506134No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17910.1537793133766537No Hit
GATTATTACTGCAGCTCATTTGCAGACAACGACTATAATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT16720.14356170405682023No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTATAGTCGTTGTCTGCAAATGAGCTG16500.14167273426659888No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16080.13806651921254No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15960.1370361720542375No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15900.1365209984750862No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15850.1360916871591268No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15720.1349754777376324No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCGGTCAC15630.1342027173689055No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15570.13368754378975423No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15520.13325823247379484No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATTATAGTCGTTGT15510.13317237021060296No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATTT15490.1330006456842192No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15370.13197029852591666No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG15270.1311116758939979No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTAATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC15160.13016719099888724No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14870.12767718536632278No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14630.1256164910497177No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC14590.12527304199695016No Hit
ACCCACTCCAATCCGTCCCTCAAGAGTCGAGTCACCATATCAGTAGACACGTCCAAGAATCAGTTCTCCCTGAAG14580.1251871797337583No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14380.12346993446992072No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14260.12243958731161819No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14000.12020716846862936No Hit
GTATTACTGTGCGAGAGATTGGGGATATTACACCCACTTAGACTGGGGCC13990.12012130620543748No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG13900.11934854583671058No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13760.11814647415202428No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13750.11806061188883242No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG13680.11745957604648927No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13510.11599991757222734No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT13410.11514129494030856No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACTAGGGTTCCCTGGCCCCAGTCTAAGTGGGTGTAATATC13290.11411094778200602No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13250.1137674987292385No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACTAGGGTTCCCTGG13100.11247956478136034No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13070.1122219779917847No Hit
GTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCCTGCTGGTGGCAGCTC12760.10956024783283648No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGCTGAAGCCTTCGGAGACCCTGTCCCTCACCTGCG12680.10887334972730146No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT12350.10603989504196946No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC12160.10440851204132379No Hit
CTCCAGGCCCTTCCCTGGGGGCTGCCGGATCCAGCCCCACCAGAAACCAC12130.10415092525174814No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC11800.10131747056641618No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC11760.10097402151364866No Hit
GTCCTGTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGCTGAAGCC11680.10028712340811365No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)