FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191825
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGAGAGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCAATGTC53510.448975310972668No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51710.43387242254525626No Hit
CTCTACTACTGTGCGAGAGTTCATCCTCCCCCAGGGAGCTGGCTCGACTA48980.4109663750970151No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46590.3909130954628406No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39980.33545193295995634No Hit
GACTTACAGTATGTCTCCGACCACCTTCGGCCAAGGGACACGACTGGACATTAATCGAACTGTGGCTGCACCAT38350.32177542843957796No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37090.3112034065403897No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35550.2982820464413819No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33300.27940343590711725No Hit
GTATGTCTCCGACCACCTTCGGCCAAGGGACACGACTGGACATTAATCGA33190.278480481614331No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG32860.27571161873597216No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG31660.265643026451031No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31530.26455226228682904No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29740.24953327879512513No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28850.2420657395171271No Hit
GGACACGACTGGACATTAATCGAACTGTGGCTGCACCATCTGTCTTCATC28210.23669582363182515No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC28180.23644410882470163No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27130.2276340905753781No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT27020.22671113628259182No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26780.2246974178256036No Hit
CTACTACTGTGCGAGAGTTCATCCTCCCCCAGGGAGCTGGCTCGACTACTGGGGCCAGGGAATCCGGGTCACCGT26510.22243198456149182No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25180.2112726281123487No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC24720.2074130010697879No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGCTCAGTCGCTTAGCAGTTATTTGAATTGGTATCAGCACAAA24110.2022947999916095No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCCTGCTACTCTGG23730.1991064124347115No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23650.19843517294904872No Hit
GAGACATACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGT23380.19616973968493698No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23330.19575021500639775No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22500.18878610534264678No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCCTGCT21850.183332284521637No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA21200.1778784637006272No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT21080.17687160447213307No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20980.17603255511505464No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20890.17527741069368405No Hit
GTCTCCACCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT20500.1720051182010782No Hit
GTATTAGATATAGTGGGTACACCTACTACAATCCCTCCCTCAAGAGTCGA20410.1712499737797076No Hit
GCTCCTGGGTCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC20370.17091435403687621No Hit
ACTTACTACTGTCAACAGACTTACAGTATGTCTCCGACCACCTTCGGCCA20120.16881673064418012No Hit
GTCGTGTCCCTTGGCCGAAGGTGGTCGGAGACATACTGTAAGTCTGTTGA19930.16722253686573113No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19820.16629958257294486No Hit
ATCTAATACTGCCGATCCATTCCAGCCCCTTCCCGGGGGACTGGCGGATC19780.16596396283011347No Hit
GTTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAATTCAAATAACTGCTA19240.16143309630188996No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC18960.15908375810207034No Hit
CAGTAGTAGAGAGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAGGGA18660.15656661003083508No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC18630.15631489522371153No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT18540.15555975080234094No Hit
GGTTTGTGCTGATACCAATTCAAATAACTGCTAAGCGACTGAGCTGCCCGGCAAGTGATGGTGACT18510.15530803599521742No Hit
GTGCAGCCACAGTTCGATTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG18340.15388165208818408No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC17880.15002202504562331No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGAGCCACTGCCA17690.1484278312671743No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTAATGTCCAGTCGTGTCC17630.14792440165292722No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17630.14792440165292722No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17310.14523944371027625No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17240.14465210916032137No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17010.14272229563904096No Hit
CCTTCGGCCAAGGGACACGACTGGACATTAATCGAACTGTGGCTGCACCA17000.14263839070333312No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCC16910.14188324628196253No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16840.14129591173200762No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT16750.14054076731063705No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16720.1402890525035135No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT16640.13961781301785078No Hit
CGACTGGACATTAATCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT16420.13777190443227824No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA16060.13475132674679588No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15970.1339961823254253No Hit
GAGTGAAATCTGTCCCAGAGCCACTGCCACTGAACCTTGATGGGACCCCA15930.13366056258259393No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15840.13290541816122334No Hit
GCACAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAAACATCCATTTTGCAAAGTGGGGTCCCATCAAGGTT15580.13072388983281943No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15530.1303043651542802No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15510.13013655528286452No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG15490.12996874541144884No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15460.1297170306043253No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15450.12963312566861745No Hit
GTCTCCTACAGATGCAGACAGGGAGGGTGGAGACTGGGTCATCTGGATGT15450.12963312566861745No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15360.12887798124724686No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15360.12887798124724686No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15190.12745159734021352No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTATGTCTCCGACCAC15030.12610911836888805No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15010.12594130849747237No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14920.12518616407610178No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14860.12468273446185472No Hit
CTACAATCCCTCCCTCAAGAGTCGAATCACCGTGGACATTGACACGTCCAAGAACCAGTTCTCCCTGAAACTGAC14840.12451492459043903No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14830.12443101965473119No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT14790.12409539991189981No Hit
CACCTGCACTGTCTCCGGTGACTCCATCAACAGTAATAATAACTACTGGG14720.1235080653619449No Hit
AACCAGGGAAAGCCCCTAAACTCCTGATCTATAAAACATCCATTTTGCAA14640.12283682587628218No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCT14570.12224949132632727No Hit
TCCCTGGTTTGTGCTGATACCAATTCAAATAACTGCTAAGCGACTGAGCTGCCCGGCAAGTGATGGTGACT14460.121326537033541No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14420.12099091729070963No Hit
GTTCAGTGGCAGTGGCTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA14300.11998405806221552No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGCTCAGT14280.11981624819079983No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGCTCTGGGACAGATTTCACT14220.11931281857655276No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC14100.11830595934805865No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14010.11755081492668806No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14010.11755081492668806No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13790.11570490634111551No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA13570.11385899775554298No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13500.11327166320558807No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT13370.11218089904138612No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGT13290.11150965955572337No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13230.11100622994147631No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13190.11067061019864492No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13120.11008327564869004No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12930.10848908187024102No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT12800.10739831770603905No Hit
CATCAAGGTTCAGTGGCAGTGGCTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTG12660.10622364860612926No Hit
ACCTTGATGGGACCCCACTTTGCAAAATGGATGTTTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTGTG12640.10605583873471358No Hit
GTCTCCGGTGACTCCATCAACAGTAATAATAACTACTGGGCCTGGATCCG12630.10597193379900574No Hit
GTCCCAGAGCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAAAT12420.10420993014914103No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12410.10412602521343318No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT12360.10370650053489396No Hit
CCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAG12210.10244792649927632No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12080.10135716233507436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)