FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191825
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGAGAGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAGGGAGAACTGGTTCTTGGACGTGTCAATGTC49270.41339961823254257No Hit
CTCTACTACTGTGCGAGAGTTCATCCTCCCCCAGGGAGCTGGCTCGACTA46660.3915004300127955No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45960.3856270845132465No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43160.36213370251505045No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40430.3392276550668093No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40300.33813689090260735No Hit
GACTTACAGTATGTCTCCGACCACCTTCGGCCAAGGGACACGACTGGACATTAATCGAACTGTGGCTGCACCAT35200.2953453736916074No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34890.29274432068466427No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG33430.2804942000713192No Hit
GTATGTCTCCGACCACCTTCGGCCAAGGGACACGACTGGACATTAATCGA32070.26908312881505253No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31580.2649717869653682No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30200.2533929058376859No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC29410.24676441591676632No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28610.24005202106013884No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28330.23770268286031926No Hit
GGACACGACTGGACATTAATCGAACTGTGGCTGCACCATCTGTCTTCATC28180.23644410882470163No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26830.22511694250414283No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25890.2172298785476056No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25730.21588739957628006No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24390.2046441381914291No Hit
CTACTACTGTGCGAGAGTTCATCCTCCCCCAGGGAGCTGGCTCGACTACTGGGGCCAGGGAATCCGGGTCACCGT24020.2015396555702389No Hit
CTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC23790.19960984204895854No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC23360.19600192981352127No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23310.19558240513498207No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22590.18954124976401737No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAGCTCAGTCGCTTAGCAGTTATTTGAATTGGTATCAGCACAAA22310.18719191156419776No Hit
GAGACATACTGTAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGT22220.18643676714282717No Hit
GTATTAGATATAGTGGGTACACCTACTACAATCCCTCCCTCAAGAGTCGA22150.1858494325928723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22100.18542990791433306No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCCTGCT21310.17880141799341345No Hit
GTCGTGTCCCTTGGCCGAAGGTGGTCGGAGACATACTGTAAGTCTGTTGA20870.17510960082226837No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT20690.1735993119795272No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20540.17234073794390956No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCCTGCTACTCTGG20480.17183730832966249No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA20180.1693201602584272No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20110.16873282570847228No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19880.1668030121871919No Hit
GGTTTGTGCTGATACCAATTCAAATAACTGCTAAGCGACTGAGCTGCCCGGCAAGTGATGGTGACT19870.16671910725148406No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19810.16621567763723702No Hit
ATCTAATACTGCCGATCCATTCCAGCCCCTTCCCGGGGGACTGGCGGATC19760.1657961529586978No Hit
ACTTACTACTGTCAACAGACTTACAGTATGTCTCCGACCACCTTCGGCCA19670.1650410085373272No Hit
GTCTCCACCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19570.1642019591802488No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19470.16336290982317037No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19280.16176871604472132No Hit
GTTTAGGGGCTTTCCCTGGTTTGTGCTGATACCAATTCAAATAACTGCTA19200.16109747655905857No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGCTCTGGGACAGATTTCACT19180.16092966668764289No Hit
GTGCAGCCACAGTTCGATTAATGTCCAGTCGTGTCCCTTGGCCGAAGGTG19040.1597549975877331No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC18300.15354603234535272No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18210.15279088792398213No Hit
GCTCCTGGGTCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCACCCTCC18200.1527069829882743No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17920.1503576447884547No Hit
GAGTGAAATCTGTCCCAGAGCCACTGCCACTGAACCTTGATGGGACCCCA17580.14750487697438802No Hit
CAGTAGTAGAGAGCCGTGTCTGCGGCGGTCACAGAGGTCAGTTTCAGGGA17530.1470853522958488No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGAGCCACTGCCA17520.14700144736014095No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT17300.1451555387745684No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGATTAATGTCCAGTCGTGTCC17240.14465210916032137No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA16720.1402890525035135No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16700.14012124263209783No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16660.13978562288926646No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16280.13659723533236842No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC16230.13617771065382922No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCTCC16150.13550647116816647No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15680.13156293918989787No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15440.12954922073290961No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15440.12954922073290961No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT15410.1292975059257861No Hit
CCTTCGGCCAAGGGACACGACTGGACATTAATCGAACTGTGGCTGCACCA15410.1292975059257861No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15290.12829064669729195No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15090.1266125479831351No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15050.12627692824030373No Hit
CACCTGCACTGTCTCCGGTGACTCCATCAACAGTAATAATAACTACTGGG15040.1261930233045959No Hit
GCACAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAAACATCCATTTTGCAAAGTGGGGTCCCATCAAGGTT14930.12527006901180962No Hit
TCCCTGGTTTGTGCTGATACCAATTCAAATAACTGCTAAGCGACTGAGCTGCCCGGCAAGTGATGGTGACT14860.12468273446185472No Hit
GTCTCCTACAGATGCAGACAGGGAGGGTGGAGACTGGGTCATCTGGATGT14770.12392759004048413No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG14750.12375978016906845No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14580.12233339626203511No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14440.12115872716212531No Hit
CGACTGGACATTAATCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT14400.12082310741929395No Hit
GTTCAGTGGCAGTGGCTCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA14370.1205713926121704No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14160.11880938896230572No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14130.11855767415518217No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14070.11805424454093512No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAGCTCAGT13880.1164600507624861No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGTATGTCTCCGACCAC13870.11637614582677826No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13760.11545319153399199No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG13720.11511757179116062No Hit
GTCCCAGAGCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAAAT13670.1146980471126214No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC13580.11394290269125082No Hit
CTACAATCCCTCCCTCAAGAGTCGAATCACCGTGGACATTGACACGTCCAAGAACCAGTTCTCCCTGAAACTGAC13550.11369118788412727No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13460.11293604346275671No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13380.11226480397709396No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13300.11159356449143121No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13250.11117403981289199No Hit
GTCTCCGGTGACTCCATCAACAGTAATAATAACTACTGGGCCTGGATCCG13060.10957984603444297No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13000.10907641642019593No Hit
AACCAGGGAAAGCCCCTAAACTCCTGATCTATAAAACATCCATTTTGCAA12970.10882470161307238No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12880.1080695571917018No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12860.10790174732028611No Hit
GTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGACTTACAGT12850.10781784238457827No Hit
CTGCTGATGGTGAGAGTGAAATCTGTCCCAGAGCCACTGCCACTGAACCT12740.10689488809179198No Hit
ACCTTGATGGGACCCCACTTTGCAAAATGGATGTTTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTGTG12660.10622364860612926No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGTCT12550.10530069431334299No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12400.10404212027772534No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT12320.1033708807920626No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT12220.10253183143498416No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT12010.10076982778511945No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGT12000.10068592284941161No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11970.10043420804228809No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11920.10001468336374886No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11920.10001468336374886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)