FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004687247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1307491
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGACTGGTCGGGTGTTCGG102480.7837912459818078No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGG99910.7641352789426467No Hit
GTATACATACTTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA80360.6146122611933849No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC65500.5009594712315419No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCGACCAGTCAGGCTGTCATCCCATG65060.4975942473026583No Hit
GTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCA64150.4906343523588308No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC60320.4613416077051391No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG58650.44856905324778523No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCGACCAGTCAGG53020.4055094834304787No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT51790.3961021529020085No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG51230.3918191406288839No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA43840.3352986750960427No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC42470.3248205914992914No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT42130.3222201911906086No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC41410.31671346112516263No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40280.3080709542168933No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG39910.30524110682215017No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT39390.3012640239971059No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT37900.28986815205611355No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG37720.2884914695397521No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG37040.28329066892238647No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG35760.27350092658381586No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC34400.26309932534908465No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG34260.26202857228080345No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA34220.2617226428327231No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC34070.2605754074024219No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC33650.25736314819757844No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA33370.25522164206101605No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG32470.24833822947920867No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32440.2481087823931484No Hit
CCCCTGAGGGCCGCTGATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATA32420.24795581766910826No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31390.2400781343810397No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG30930.2365599457281159No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29940.22898819188812772No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCCGACCAGTCAGGC27700.2118561427956292No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC27440.20986760138310703No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA27330.20902629540088613No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA27160.20772609524654473No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA25920.19824228235605446No Hit
GCATGGGATGACAGCCTGACTGGTCGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGG25900.1980893176320143No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC25190.19265906992858842No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC24580.18799364584536338No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24520.18753475167324288No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC24330.1860815867948613No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT23970.18332822176213834No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT23860.1824869157799174No Hit
GCCTGGTCCCTCCGCCGAACACCCGACCAGTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCTT23680.18111023326355594No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAC23150.17705666807649154No Hit
GGCCATCAGTGGGCTCCGGTCCGAAGATGAGGCTGATTATTACTGTGCAG23070.17644480918033087No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22990.17583295028417023No Hit
GATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG22600.172850138165387No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT22360.171014561476905No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA21350.16328984291287665No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG21340.16321336055085656No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGCCCAC21290.16283094874075615No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC21280.16275446637873606No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGAGACAATGCCAAGAACTCACTGTATCTG21080.16122481913833442No Hit
GTGCAGCATGGGATGACAGCCTGACTGGTCGGGTGTTCGGCGGAGGGACC20740.15862441882965161No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20670.15808904229551102No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20390.1559475361589487No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20340.1555651243488483No Hit
ATGCTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATG20240.15480030072864748No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAAGATG19890.15212341805794458No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19840.15174100624784415No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19840.15174100624784415No Hit
GGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG19570.1496759824733019No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19390.14829929995694044No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC19330.14784040578481994No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC19220.146999099802599No Hit
GGATGACAGCCTGACTGGTCGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19180.14669317035451868No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG18870.14432221713189614No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC18850.14416925240785597No Hit
GTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCTTCGGA18690.14294553461553464No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG18460.14118644028907273No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18190.1391214165145305No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAAGTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCC17990.13759176927412886No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17350.13269689810484356No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17320.1324674510187833No Hit
GTGTATTACTGTGCGAGAGATTTAGCCTCCAGAATGGCTGATATCTGGGG17220.13170262739858246No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG17120.13093780377838166No Hit
CTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACATACTTACTTCCGATGTTGGAG17010.13009649779616075No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG17000.13002001543414066No Hit
CTATAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC16880.12910222708989966No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16560.12665479150525702No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC16540.12650182678121685No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTC16480.12604293260909635No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG16270.12443680300667462No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG16200.12390142647253402No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG16180.12374846174849387No Hit
CATCTATAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTC16160.12359549702445372No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT16120.12328956757637337No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT15880.1214539908878914No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG15850.12122454380183113No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACATACTTACTT15780.12068916726769056No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15720.12023027309557006No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG15500.11854766113112825No Hit
GTCAGGGACCCCTGAGGGCCGCTGATCATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTA15500.11854766113112825No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC15500.11854766113112825No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15480.11839469640708808No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15400.11778283751092743No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG15330.11724746097678683No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC15280.11686504916668641No Hit
CAGCCTGACTGGTCGGGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT15130.11571781373638519No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGC15090.11541188428830484No Hit
GGTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG15080.11533540192628478No Hit
GCACAGTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG15050.11510595484022451No Hit
GTAATAATCAGCCTCATCTTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA15030.11495299011618436No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA15000.11472354303012412No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA14970.11449409594406386No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG14820.11334686051376261No Hit
TTACTGTGCAGCATGGGATGACAGCCTGACTGGTCGGGTGTTCGGCGGAG14740.11273500161760196No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT14650.11204666035942121No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG14560.11135831910124047No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG14490.1108229425670999No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC14280.10921681296467815No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG14070.1076106833622564No Hit
GTCATCCCATGCTGCACAGTAATAATCAGCCTCATCTTCGGACCGGAGCC14070.1076106833622564No Hit
GATGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13960.10676937738003549No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC13960.10676937738003549No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA13790.10546917722569409No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG13790.10546917722569409No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT13670.1045513888814531No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGG13570.10378656526125228No Hit
GCTGGTACCAGTATACATACTTACTTCCGATGTTGGAGCTGCTTCCAGAA13380.1023334003828707No Hit
GGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCT13330.10195098857277028No Hit
CAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG13250.10133912967660963No Hit
CCAGTATACATACTTACTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGA13240.10126264731458955No Hit
GAATATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13170.10072727078044898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)