FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551998
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATCACTGTGCAACATGGGATGACAGTGTGAGTGGTCCGGGTGTTGT81720.5265470702926164No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC81270.52364758202008No Hit
GTGATAATCAGCCTCGTCCTCGGACCGGAGCCCACTGATGGCCAGGGTGG72630.4679774071873804No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57230.36875047519391135No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCCGGACCACTCACACTGTCATCC53320.3435571437592059No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG49170.31681741857914764No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG48950.315399890979241No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45720.2945880084897017No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT45360.2922684178716725No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCCGGACCA43100.27770654343626733No Hit
TTCCTGTACATGACGACTTTGGGGGCCGTTCCTGGGAATTGTTGGTACCA43080.2775776772908212No Hit
GTACAGGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCACC42170.27171426767302537No Hit
GTGCAGGGTCGTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT40770.26269363749180086No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG39470.2543173380378067No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38510.24813176305639567No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC38490.24800289691094962No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT38040.2451034086384132No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT37680.24278381802038404No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG37400.24097969198413915No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC34090.21965234491281563No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33580.216366258203941No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32900.21198480925877483No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32620.21018068322252995No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31660.20399510824111886No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31230.2012244861140285No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG30490.19645643873252416No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30120.19407241504177195No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC29880.1925260212964192No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28800.18556724944233177No Hit
CAGTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC27560.1775775484246758No Hit
GGATGACAGTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG27490.1771265169156146No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26140.16842805209800527No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC25960.1672682567889907No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC25710.1656574299709149No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC25450.1639821700801161No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24040.15489710682616858No Hit
GTGCAACATGGGATGACAGTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGA23730.1528996815717546No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC23640.1523197839172473No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23510.15148215397184792No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA23430.15096668939006364No Hit
CTCACACTGTCATCCCATGTTGCACAGTGATAATCAGCCTCGTCCTCGGA23100.1488403979902036No Hit
CCCCTGAGGGCCGCTGATCATTCCTGTACATGACGACTTTGGGGGCCGTTCCTGGGAATTGTTGGTACCAGTATA22790.1468429727357896No Hit
GGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG22700.14626307508128233No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT21340.13750017719094998No Hit
GATCATTCCTGTACATGACGACTTTGGGGGCCGTTCCTGGGAATTGTTGG21340.13750017719094998No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21200.13659811417282755No Hit
CCTCACTCACTGTGCAGGGTCGTGGGCCCAGTCTGTGCTGACTCAGCCAC21000.13530945271836692No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG20940.13492285428202871No Hit
ATGTTGCACAGTGATAATCAGCCTCGTCCTCGGACCGGAGCCCACTGATG20870.1344718227729675No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20690.1333120274639529No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGACGAGGCTGATTATCACTGTGCAA20430.1316367675731541No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT20390.13137903528226197No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC20280.13067027148230861No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20240.1304125391914165No Hit
CTGTCATCCCATGTTGCACAGTGATAATCAGCCTCGTCCTCGGACCGGAG19940.12847954700972553No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT19670.12673985404620366No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCCGGACCAC19610.12635325560986546No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19570.12609552331897336No Hit
TGGGATGACAGTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGAGGGACCAA19550.12596665717352729No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19520.12577335795535818No Hit
CTGATGGCCAGGGTGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC19500.12564449180991213No Hit
ACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCCGGCCATG19390.12493572800995878No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC19240.12396923191911331No Hit
GTCATCCCATGTTGCACAGTGATAATCAGCCTCGTCCTCGGACCGGAGCC19200.12371149962822117No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCACCCTGGCCATCAGTGGGCTCCGGT19070.12287386968282175No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18900.12177850744653021No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA18510.11926561761033197No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18500.11920118453760895No Hit
GTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT18210.11733262542864102No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18210.11733262542864102No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17960.11572179861056521No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17650.11372437335615124No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT17620.11353107413798214No Hit
GTATACATAATTACGTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA17410.11217797961079846No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACAC17370.11192024731990634No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT17360.11185581424718331No Hit
GTCGTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC17320.11159808195629119No Hit
CAGCCACCCTGGCCATCAGTGGGCTCCGGTCCGAGGACGAGGCTGATTAT16940.10914962519281597No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT16460.10605683770211044No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC16430.10586353848394134No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16420.10579910541121831No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16280.10489704239309587No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG16090.10367281401135826No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA15790.10173982182966732No Hit
GTCCGGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT15700.10115992417516002No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15550.10019342808431456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)