FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551998
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATCACTGTGCAACATGGGATGACAGTGTGAGTGGTCCGGGTGTTGT77520.4994851797489429No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC76560.4932996047675319No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT55060.35476849841301344No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53120.3422684823047452No Hit
GTGCAGGGTCGTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT49080.3162375209246404No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCCGGACCACTCACACTGTCATCC46940.3024488433619116No Hit
GTGATAATCAGCCTCGTCCTCGGACCGGAGCCCACTGATGGCCAGGGTGG46420.29909832358031385No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC45980.2962632683805005No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG45050.2902709926172585No Hit
GTACAGGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCACC45010.2900132603263664No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG43410.2797039686906813No Hit
TTCCTGTACATGACGACTTTGGGGGCCGTTCCTGGGAATTGTTGGTACCA42560.2742271575092236No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT40780.26275807056452394No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG40240.2592786846374802No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38590.24864722763817992No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37630.2424616526567689No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCCGGACCA37610.24233278651132284No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36820.2372425737662033No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG35740.2302838019121159No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC34520.222422967039906No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC33540.21610852591304885No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT32090.20676573036820925No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG31770.2047038720410722No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA31680.20412397438656493No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31290.2016110845503667No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA30570.19697190331430836No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT29910.1927193205145883No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG29390.18936880073299064No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC29260.1885311707875912No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA28530.1838275564788099No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28500.1836342572606408No Hit
CAGTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC27610.17789971378829095No Hit
GGATGACAGTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG27540.17744868227922975No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC27260.17564455624298486No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26910.17338939869767875No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26670.171843004952326No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26650.1717141388068799No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC25350.16333783935288576No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG24100.1552837052625068No Hit
GGAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG23770.15315741386264672No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23570.1518687524081861No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23550.15173988626274004No Hit
GTGCAACATGGGATGACAGTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGA23140.14909813028109573No Hit
CCTCACTCACTGTGCAGGGTCGTGGGCCCAGTCTGTGCTGACTCAGCCAC22460.14471668133592958No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22350.1440079175359762No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC22280.143556886026915No Hit
CCCCTGAGGGCCGCTGATCATTCCTGTACATGACGACTTTGGGGGCCGTTCCTGGGAATTGTTGGTACCAGTATA21650.13949760244536397No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC21450.13820894099090333No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20770.13382749204573718No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT20630.13292542902761473No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCACCCTGGCCATCAGTGGGCTCCGGT20620.1328609959548917No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG20390.13137903528226197No Hit
GTCGTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC20220.1302836730459704No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA19790.12751305091888004No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19740.1271908855552649No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGACGAGGCTGATTATCACTGTGCAA19670.12673985404620366No Hit
GTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT19280.12422696421000541No Hit
GATCATTCCTGTACATGACGACTTTGGGGGCCGTTCCTGGGAATTGTTGG19270.12416253113728239No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCGTGGGC19120.12319603504643692No Hit
TGGGATGACAGTGTGAGTGGTCCGGGTGTTGTTTTCGGCGGAGGGACCAA18850.12145634208291504No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT18670.12029654677390048No Hit
CTGATGGCCAGGGTGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18670.12029654677390048No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18610.11990994833756229No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG18200.117268192355918No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC17870.11514190095605795No Hit
GGGTGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCT17820.11481973559244278No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA17820.11481973559244278No Hit
CTCACACTGTCATCCCATGTTGCACAGTGATAATCAGCCTCGTCCTCGGA17540.1130156095561979No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG17320.11159808195629119No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT17260.111211483519953No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGACGTAATTATGTATACTGGTACCAACAATTCCCAGGAACGGCCCC17010.10960065670187719No Hit
ACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCCGGCCATG16890.1088274598292008No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG16740.10786096373835534No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACACCCGGACCAC16730.10779653066563229No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACAACAC16480.1061857038475565No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC16480.1061857038475565No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC16430.10586353848394134No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16360.10541250697488012No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT16340.10528364082943405No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC16250.10470374317492677No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC16220.10451044395675768No Hit
GTCTGGCACCTCAGCCACCCTGGCCATCAGTGGGCTCCGGTCCGAGGACG16130.10393054630225039No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16000.10309291635685097No Hit
GTCCGGGTGTTGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT15970.10289961713868187No Hit
GTCATCCCATGTTGCACAGTGATAATCAGCCTCGTCCTCGGACCGGAGCC15760.10154652261149821No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15750.10148208953877519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14250.042.1697161
AAATCGA350.001280794639.84056545
GTATCAA36350.039.2506681
CGCGAAA400.00249723834.77554344
GTGGTAT4100.034.3896641
CGTCTAT400.002915862233.69355428
TATCAAC43550.032.447892
GCCAGAG23100.032.11694770
ATCAACG44500.031.6777133
TCAACGC44750.031.5757474
CAACGCA44950.031.3636155
AACGCAG45100.031.1999556
GAGTTTA4850.031.1482221
TATTAGA1550.030.3171842
AGAGTAC46400.030.27393511
CAGAGTA46550.030.17151510
TGGTATC4800.030.0690542
ACGCAGA47100.029.9473827
CGCAGAG47350.029.7921478
CTAATGG950.005360588429.75043370
Produced by FastQC (version 0.11.5)