FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004687253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008343
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47370.4697806202849626No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40030.3969879297024921No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTTTGCAGGGCCTCTCAGAGTGTTAGCGG35260.3496825980841836No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAACCTAGAGCCTGAAGATT34630.34343472409685993No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCT32170.31903826376540523No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31820.31556722266133647No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31060.3080301048353586No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28500.28264191847417No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27200.26974948008762895No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGTGGCCCTATT26740.26518754035085285No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25750.2553694526564869No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25070.24862571565429623No Hit
GTGCAGCCACAGTTCGGTTGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCACCGGAGGCCAGTTGCTTCG24260.2405927348134514No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24160.23960100878371746No Hit
GAATAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAA22760.22571684436744244No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22530.2234358744990544No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21860.21679131009983707No Hit
GACTTCACTCTCACCATCAGCAACCTAGAGCCTGAAGATTTTGCGGTTTATTACTGTCAGCAGCGAAGCAACTGG21240.21064260871548668No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20790.20617984158168404No Hit
GTTTATTACTGTCAGCAGCGAAGCAACTGGCCTCCGGTGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCAAC19840.19675844429921166No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19710.19546920046055755No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19670.195072510048664No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19270.19110560592972828No Hit
CATCTATGATGCATCGAATAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA19120.1896180168851274No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCACCGGAGG18780.18624614838403203No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCGAATAGGGCCACTGACATCCCAGCCAGGTT18490.18337014289780362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18480.18327097029483022No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17960.1781139949402138No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTTTGCAGGGCCTCTCAGAGTGTTAGCGGCTACTTAGCCTGGTA17880.17732061411642666No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17160.17018018670234236No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17160.17018018670234236No Hit
GTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCGAATAGGGCCACTGACATCCCAGC15870.15738692091877465No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT15430.15302332638794536No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCA15140.15014732090171698No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15050.14925476747495645No Hit
ACCTAGAGCCTGAAGATTTTGCGGTTTATTACTGTCAGCAGCGAAGCAAC14960.14836221404819588No Hit
CTGCTACTCTGGCTCCCAGATATCTCCGGAGAGATTGTTTTGACACAGTC14890.14766800582738215No Hit
GTAATAAACCGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA14300.14181682225195197No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14280.14161847704600516No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGC14060.13943667978059052No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13890.13775074553004285No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13790.13675901950030894No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13730.13616398388246856No Hit
CTTTTGCAGGGCCTCTCAGAGTGTTAGCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCT13260.1315028715427191No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13260.1315028715427191No Hit
GTTTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC13240.1313045263367723No Hit
GCCCTATTCGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGCTAAGTAGCCG13090.12981693729217142No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12920.12813100304162373No Hit
CAGTAATAAACCGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12690.1258500331732357No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12450.12346989070187427No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12390.12287485508403391No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT12390.12287485508403391No Hit
GGTTTGTGTTGGTACCAGGCTAAGTAGCCGCTAACACTCTGAGAGGCCCTGCAAAAGAGGGTGGCTCTTTCCC12100.1199988495978055No Hit
CTCAGAGTGTTAGCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG12070.11970133178888533No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT11990.11890795096509818No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAACC11960.118610433156178No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11760.11662698109671016No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT11720.11623029068481658No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11680.11583360027292301No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11640.11543690986102943No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11570.11474270164021569No Hit
CTCCCAGGCTCCTCATCTATGATGCATCGAATAGGGCCACTGACATCCCA11410.1131559399926414No Hit
GTGTTAGCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCC11380.11285842218372122No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC11280.11186669615398728No Hit
TTATTACTGTCAGCAGCGAAGCAACTGGCCTCCGGTGCTCACTTTCGGCG11160.11067662491830656No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11090.10998241669749281No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11040.10948655368262586No Hit
ACCGTGGTCCCTCCGCCGAAAGTGAGCACCGGAGGCCAGTTGCTTCGCTG11000.10908986327073228No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10960.10869317285883871No Hit
GATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCACCGGAGGCCAGTTGC10930.10839565504991854No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10860.10770144682910476No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT10700.1061146851815305No Hit
GACCACGGTTGAGATCAACCGAACTGTGGCTGCACCATCTGTCTTCATCT10690.10601551257855708No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC10560.10472626873990298No Hit
GTTGAGATCAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC10490.10403206051908924No Hit
CCGGAGGCCAGTTGCTTCGCTGCTGACAGTAATAAACCGCAAAATCTTCA10380.10294116188638192No Hit
TCTCCGGAGAGATTGTTTTGACACAGTCTCCAGCCACCCTGTCTTTGTCT10340.10254447147448834No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10180.10095770982691407No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCTCCGGAGAGATTGTTTTGACACAGTCTC10130.10046184681204709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)