FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004687254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004687254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008343
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47500.4710698641236167No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAACCTAGAGCCTGAAGATT35920.3562279898804276No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTTTGCAGGGCCTCTCAGAGTGTTAGCGG35590.35295529398230563No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34230.3394678199779242No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30980.3072367240115714No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30350.3009888500242477No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCT29620.29374925000719No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28020.2778816335314471No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27560.2733196937946711No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCAGTGGCCCTATT25630.2541793814208062No Hit
GTGCAGCCACAGTTCGGTTGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCACCGGAGGCCAGTTGCTTCG25290.2508075129197109No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25080.2487248882572696No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23780.23583244987072852No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22540.2235350471020278No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22350.22165076764553332No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22200.22016317860093243No Hit
GACTTCACTCTCACCATCAGCAACCTAGAGCCTGAAGATTTTGCGGTTTATTACTGTCAGCAGCGAAGCAACTGG20530.2036013539043758No Hit
GAATAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAA20200.20032865800625382No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20170.20003114019733365No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19650.19487416484271722No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19230.1907089155178347No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19170.19011387989999434No Hit
GTTTATTACTGTCAGCAGCGAAGCAACTGGCCTCCGGTGCTCACTTTCGGCGGAGGGACCACGGTTGAGATCAAC18740.18584945797213845No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTGATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCACCGGAGG18640.18485773194240454No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18060.17910572096994773No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18010.17860985795508075No Hit
CATCTATGATGCATCGAATAGGGCCACTGACATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17700.1755355072629056No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTTTGCAGGGCCTCTCAGAGTGTTAGCGGCTACTTAGCCTGGTA17280.17137025793802307No Hit
ACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCGAATAGGGCCACTGACATCCCAGCCAGGTT17190.17047770451126254No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16410.16274224147933788No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16120.1598662359931095No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15780.15649436749201412No Hit
CTGCTACTCTGGCTCCCAGATATCTCCGGAGAGATTGTTTTGACACAGTC15730.15599850447714717No Hit
GTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCGAATAGGGCCACTGACATCCCAGC15360.15232911816713163No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGTCA15270.1514365647403711No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15190.15064318391658393No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14960.14836221404819588No Hit
ACCTAGAGCCTGAAGATTTTGCGGTTTATTACTGTCAGCAGCGAAGCAAC14880.14756883322440878No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14700.14578372637088768No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14600.14479200034115375No Hit
GCCCTATTCGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGCTAAGTAGCCG14440.14320523869357946No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13930.13814743594193643No Hit
GGTGTACACCCTGCCCCCATCCCAGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13740.13626315648544196No Hit
GTAATAAACCGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13600.13487474004381447No Hit
GTTTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC13570.13457722223489427No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13270.1316020441456925No Hit
CTTTTGCAGGGCCTCTCAGAGTGTTAGCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCT13220.13110618113082553No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGC12870.12763514002675674No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12810.12704010440891642No Hit
GGTTTGTGTTGGTACCAGGCTAAGTAGCCGCTAACACTCTGAGAGGCCCTGCAAAAGAGGGTGGCTCTTTCCC12510.12406492631971464No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12300.12198230165727338No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12220.12118892083348623No Hit
GTGTTAGCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCC12050.11950298658293854No Hit
CTCAGAGTGTTAGCGGCTACTTAGCCTGGTACCAACACAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG11820.1172220167145505No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAACC11760.11662698109671016No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11220.11127166053614693No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11180.11087497012425335No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11080.10988324409451942No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11070.10978407149154602No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11030.10938738107965246No Hit
CAGTAATAAACCGCAAAATCTTCAGGCTCTAGGTTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10940.10849482765289192No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10820.10730475641721121No Hit
CTCCCAGGCTCCTCATCTATGATGCATCGAATAGGGCCACTGACATCCCA10770.10680889340234424No Hit
TTATTACTGTCAGCAGCGAAGCAACTGGCCTCCGGTGCTCACTTTCGGCG10710.10621385778450387No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10640.10551964956369013No Hit
GATCTCAACCGTGGTCCCTCCGCCGAAAGTGAGCACCGGAGGCCAGTTGC10600.10512295915179656No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10560.10472626873990298No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC10540.1045279235339562No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAT10540.1045279235339562No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10540.1045279235339562No Hit
ACCGTGGTCCCTCCGCCGAAAGTGAGCACCGGAGGCCAGTTGCTTCGCTG10480.10393288791611585No Hit
CCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCTCCGGAGAGATTGTTTTGACACAGTCTC10330.10244529887151496No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10310.10224695366556817No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA10260.10175109065070119No Hit
GTGTTGGTACCAGGCTAAGTAGCCGCTAACACTCTGAGAGGCCCTGCAAAAGAGGGTGGCTCTTTCCCCTGGAGA10230.10145357284178103No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA10200.10115605503286083No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10180.10095770982691407No Hit
GTTGAGATCAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCC10160.10075936462096728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC652.237357E-1047.558736
GGTATCA8050.043.8928761
GTATCAA20700.040.704081
CGGTACC801.5224941E-939.11026845
AACGGCC801.6934791E-938.68607737
TTAACGG801.7262209E-938.61046235
CGTGCAA852.224624E-937.62328757
GCGGTAC958.247298E-932.89988744
TATCAAC25450.032.698452
ATCAACG26100.031.8825383
TCAACGC26450.031.8443054
CAACGCA26400.031.652035
AATGGTA3200.031.4042170
TACCTAG553.3579223E-431.20037735
AACGCAG26800.031.1811586
CCTTTGT6050.031.0062570
GTGGTAT2350.030.3604341
TGTTAGA13350.030.11040370
TGGTATC2500.029.8903872
AATACGT803.104642E-629.73677810
Produced by FastQC (version 0.11.5)